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UniProtKB/Swiss-Prot entry O27579

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name DP2L_METTH
Primary accession number O27579
Secondary accession numbers None
Integrated into Swiss-Prot on April 27, 2001
Sequence was last modified on January 1, 1998 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 52)
Name and origin of the protein
Protein name DNA polymerase II large subunit
Synonyms Pol II
EC 2.7.7.7
Gene name
Name: polC
OrderedLocusNames: MTH_1536
From
Methanobacterium thermoautotrophicum [TaxID: 187420] [HAMAP proteome]
Taxonomy Archaea; Euryarchaeota; Methanobacteria; Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
Protein existence 3: Inferred from homology;
References
[1]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=Delta H;
PubMed=9371463 [NCBI, ExPASy, EBI, Israel, Japan]
Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R., Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N.;
"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics.";
J. Bacteriol. 179:7135-7155(1997).
Comments
  • FUNCTION: Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase (By similarity).
  • CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
  • CATALYTIC ACTIVITY: Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates.
  • SUBUNIT: Heterodimer of a large subunit and a small subunit (By similarity).
  • SIMILARITY: Belongs to the archaeal DNA polymerase II family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AE000666; AAB86010.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR H69071; H69071.
RefSeq NP_276649.1; -.
3D structure databases
ModBase O27579.
Enzyme and pathway databases
BioCyc MTHE187420:MTH1536-MON; -.
Ontologies
GO
GO:0008408; Molecular function: 3'-5' exonuclease activity (inferred from electronic annotation from HAMAP).
GO:0003677; Molecular function: DNA binding (inferred from electronic annotation from HAMAP).
GO:0003887; Molecular function: DNA-directed DNA polymerase activity (inferred from electronic annotation from HAMAP).
GO:0000738; Biological process: DNA catabolic process, exonucleolytic (inferred from electronic annotation from HAMAP).
GO:0006261; Biological process: DNA-dependent DNA replication (inferred from electronic annotation from HAMAP).
QuickGo view.
Family and domain databases
HAMAP MF_00324; -; 1.
PBIL [Tree]
InterPro IPR004475; PolC_DP2.
IPR016033; PolC_DP2_N.
Graphical view of domain structure.
Pfam PF03833; PolC_DP2; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF016275; PolC_DP2; 1.
TIGRFAMs TIGR00354; polC; 1.
BLOCKS O27579.
Genome annotation databases
GeneID 1471805; -.
GenomeReviews AE000666_GR; MTH_1536.
KEGG mth:MTH1536; -.
NMPDR fig|187420.1.peg.1504; -.
Phylogenomic databases
HOGENOM O27579; -.
Genome annotation databases
CMR O27579; MTH_1536.
Other
ProtoNet O27579.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; DNA replication; DNA-binding; DNA-directed DNA polymerase; Exonuclease; Hydrolase; Multifunctional enzyme; Nuclease; Nucleotidyltransferase; Transferase.
Features
SEVIEWER logo Feature table viewer
KeyFrom To Length Description FTId
CHAIN   1   1092  1092     DNA polymerase II large subunit. PRO_0000152578
Sequence information
Length: 1092 AA [This is the length of the unprocessed precursor] Molecular weight: 123060 Da [This is the MW of the unprocessed precursor] CRC64: AA6970F7A6F42DFF [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MMDYFNELER ETERLYEIAR KARARGLDVS TTPEIPLAKD LAERVEGLVG PEGIARRIKE 

        70         80         90        100        110        120 
LEGDRGREEV AFQIAAEIAS QAVPDDDPEE REKLADQALR TALAILTEGV VAAPLEGIAR 

       130        140        150        160        170        180 
VRIKENFDKS RYLAVYFAGP IRSAGGTAAA LSVLIADYIR LAVGLDRYKP VEREIERYVE 

       190        200        210        220        230        240 
EVELYESEVT NLQYSPKPDE VRLAASKIPV EVTGEPTDKV EVSHRDLERV ETNNIRGGAL 

       250        260        270        280        290        300 
LAMVEGVIQK APKVLKYAKQ LKLEGWDWLE KFSKAPKKGE GEEKVVVKAD SKYVEDIIGG 

       310        320        330        340        350        360 
RPVLAYPSEK GAFRLRYGRA RNTGLAAMGV HPATMELLQF LAVGTQMKIE RPGKGNCVVP 

       370        380        390        400        410        420 
VDTIDGPVVK LRNGDVIRIE DAETASRVRS EVEEILFLGD MLVAFGEFLR NNHVLMPAGW 

       430        440        450        460        470        480 
CEEWWIQTIL SSPKYPGDDP LNLSYYRTRW NELEVSAGDA FRISEEYDVP LHPRYTYFYH 

       490        500        510        520        530        540 
DVTVRELNML REWLNTSQLE DELVLELRPE KRILEILGVP HRVKDSRVVI GHDDAHALIK 

       550        560        570        580        590        600 
TLRKPLEDSS DTVEALNRVS PVRIMKKAPT YIGTRVGRPE KTKERKMRPA PHVLFPIGKY 

       610        620        630        640        650        660 
GGSRRNIPDA AKKGSITVEI GRATCPSCRV SSMQSICPSC GSRTVIGEPG KRNINLAALL 

       670        680        690        700        710        720 
KRAAENVSVR KLDEIKGVEG MISAEKFPEP LEKGILRAKN DVYTFKDATI RHDSTDLPLT 

       730        740        750        760        770        780 
HFTPREVGVS VERLRELGYT RDCYGDELED EDQILELRVQ DVVISEDCAD YLVRVANFVD 

       790        800        810        820        830        840 
DLLERFYDLE RFYNVKTRED LVGHLIAGLA PHTSAAVLGR IIGFTGASAC YAHPYFHSAK 

       850        860        870        880        890        900 
RRNCDSDEDS VMLLLDALLN FSKSYLPSSR GGSMDAPLVL STRIDPEEID DESHNIDTMD 

       910        920        930        940        950        960 
MIPLEVYERS FDHPRPSEVL DVIDNVEKRL GKPEQYTGLM FSHNTSRIDE GPKVCLYKLL 

       970        980        990       1000       1010       1020 
PTMKEKVESQ ITLAEKIRAV DQRSVVEGVL MSHFLPDMMG NIRAFSRQKV RCTKCNRKYR 

      1030       1040       1050       1060       1070       1080 
RIPLSGECRC GGNLVLTVSK GSVIKYLEIS KELASRYPID PYLMQRIEIL EYGVNSLFES 

      1090 
DRSKQSSLDV FL
O27579 in FASTA format

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