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UniProtKB/Swiss-Prot entry O14802


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name RPC1_HUMAN
Primary accession number O14802
Secondary accession numbers Q8IW34 Q8TCW5
Integrated into Swiss-Prot on July 15, 1998
Sequence was last modified on September 23, 2008 (Sequence version 2)
Annotations were last modified on    December 16, 2008 (Entry version 72)
Name and origin of the protein
Protein name DNA-directed RNA polymerase III subunit RPC1
Synonyms RNA polymerase III subunit C1
EC 2.7.7.6
DNA-directed RNA polymerase III subunit A
DNA-directed RNA polymerase III largest subunit
RPC155
RNA polymerase III subunit C160
Gene name
Name: POLR3A
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
PubMed=9331371 [NCBI, ExPASy, EBI, Israel, Japan]
Sepehri S., Hernandez N.;
"The largest subunit of human RNA polymerase III is closely related to the largest subunit of yeast and trypanosome RNA polymerase III.";
Genome Res. 7:1006-1019(1997).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1038/nature02462; PubMed=15164054 [NCBI, ExPASy, EBI, Israel, Japan]
Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J., Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.;
"The DNA sequence and comparative analysis of human chromosome 10.";
Nature 429:375-381(2004).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.;
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Uterus;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[5]
NUCLEOTIDE SEQUENCE [MRNA] OF 1-374.
DOI=10.1002/art.10521; PubMed=12384934 [NCBI, ExPASy, EBI, Israel, Japan]
Kuwana M., Kimura K., Kawakami Y.;
"Identification of an immunodominant epitope on RNA polymerase III recognized by systemic sclerosis sera: application to enzyme-linked immunosorbent assay.";
Arthritis Rheum. 46:2742-2747(2002).
[6]
IDENTIFICATION IN THE RNA POL III COMPLEX, AND MASS SPECTROMETRY.
DOI=10.1128/MCB.22.22.8044-8055.2002; PubMed=12391170 [NCBI, ExPASy, EBI, Israel, Japan]
Hu P., Wu S., Sun Y., Yuan C.-C., Kobayashi R., Myers M.P., Hernandez N.;
"Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits.";
Mol. Cell. Biol. 22:8044-8055(2002).
Comments
  • FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity).
  • CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
  • SUBUNIT: Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits (By similarity).
  • SUBCELLULAR LOCATION: Nucleus.
  • SIMILARITY: Belongs to the RNA polymerase beta' chain family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF021351; AAB86536.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL512628; CAI16466.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
CH471083; EAW54617.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC041089; AAH41089.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY091459; AAM12029.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_008986.2; -.
UniGene Hs.436896
3D structure databases
HSSP P04050; 1I6H. [HSSP ENTRY / PDB]
ModBase O14802.
PTM databases
PhosphoSite O14802; -.
Enzyme and pathway databases
Reactome REACT_1788; Transcription.
Organism-specific databases
GeneCards GC10M079405; -.
H-InvDB HIX0008960; -.
HGNC HGNC:30074; POLR3A.
GenAtlas POLR3A.
PharmGKB PA134900426; -.
GeneCards O14802.
Gene expression databases
ArrayExpress O14802; -.
CleanEx HS_POLR3A; -.
GermOnline ENSG00000148606; Homo sapiens.
Ontologies
GO
GO:0005666; Cellular component: DNA-directed RNA polymerase III complex (non-traceable author statement from UniProtKB).
GO:0003677; Molecular function: DNA binding (inferred from electronic annotation from InterPro).
GO:0003899; Molecular function: DNA-directed RNA polymerase activity (non-traceable author statement from UniProtKB).
GO:0000287; Molecular function: magnesium ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0008270; Molecular function: zinc ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0006351; Biological process: transcription, DNA-dependent (non-traceable author statement from UniProtKB).
QuickGo view.
Family and domain databases
InterPro IPR015700; RNA_pol3.
IPR000722; RNA_pol_A.
IPR006592; RNA_pol_N.
IPR007080; RNA_pol_Rpb1_1.
IPR007066; RNA_pol_Rpb1_3.
IPR007083; RNA_pol_Rpb1_4.
IPR007081; RNA_pol_Rpb1_5.
Graphical view of domain structure.
Gene3D G3DSA:2.40.40.30; RNA_pol_A; 1.
G3DSA:3.90.1120.10; RNA_pol_Rpb1_1; 1.
PANTHER PTHR19376:SF15; RNA_pol3; 1.
Pfam PF04997; RNA_pol_Rpb1_1; 1.
PF00623; RNA_pol_Rpb1_2; 1.
PF04983; RNA_pol_Rpb1_3; 1.
PF05000; RNA_pol_Rpb1_4; 1.
PF04998; RNA_pol_Rpb1_5; 1.
Pfam graphical view of domain structure.
SMART SM00663; RPOLA_N; 1.
SMART graphical view of domain structure.
Proteomics databases
PRIDE O14802; -.
Genome annotation databases
Ensembl ENSG00000148606; Homo sapiens. [Contig view]
GeneID 11128; -.
Phylogenomic databases
HOGENOM O14802; -.
HOVERGEN O14802; -.
Other
ProtoNet O14802.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
DNA-directed RNA polymerase; Magnesium; Metal-binding; Nucleotidyltransferase; Nucleus; Polymorphism; Transcription; Transferase; Zinc; Zinc-finger.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom    To Length Description FTId
CHAIN   1   1390  1390     DNA-directed RNA polymerase III subunit RPC1. PRO_0000073947
ZN_FING   69     82  14     C3H1-type (Potential). 
REGION   844    856  13     Bridging helix (By similarity). 
METAL   69     69        Zinc 1 (By similarity). 
METAL   72     72        Zinc 1 (By similarity). 
METAL   79     79        Zinc 1 (By similarity). 
METAL   82     82        Zinc 1 (By similarity). 
METAL   109    109        Zinc 2 (By similarity). 
METAL   112    112        Zinc 2 (By similarity). 
METAL   156    156        Zinc 2 (By similarity). 
METAL   499    499        Magnesium; catalytic (By similarity). 
METAL   501    501        Magnesium; catalytic (By similarity). 
METAL   503    503        Magnesium; catalytic (By similarity). 
VARIANT   582    582  1     R -> L (in dbSNP:rs34588967 [NCBI]). VAR_051873 
VARIANT   713    713  1     K -> N (in dbSNP:rs35354908 [NCBI]). VAR_051874 
CONFLICT   15     15        I -> T (in Ref. 1; AAB86536). 
CONFLICT   262    262        I -> F (in Ref. 1; AAB86536). 
CONFLICT   283    283        L -> P (in Ref. 5; AAM12029). 
CONFLICT   1056   1056        A -> G (in Ref. 1; AAB86536). 
CONFLICT   1275   1275        M -> MV (in Ref. 1; AAB86536). 
Sequence information
Length: 1390 AA [This is the length of the unprocessed precursor] Molecular weight: 155641 Da [This is the MW of the unprocessed precursor] CRC64: 56F30900848E3DB9 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MVKEQFRETD VAKKISHICF GMKSPEEMRQ QAHIQVVSKN LYSQDNQHAP LLYGVLDHRM 

        70         80         90        100        110        120 
GTSEKDRPCE TCGKNLADCL GHYGYIDLEL PCFHVGYFRA VIGILQMICK TCCHIMLSQE 

       130        140        150        160        170        180 
EKKQFLDYLK RPGLTYLQKR GLKKKISDKC RKKNICHHCG AFNGTVKKCG LLKIIHEKYK 

       190        200        210        220        230        240 
TNKKVVDPIV SNFLQSFETA IEHNKEVEPL LGRAQENLNP LVVLNLFKRI PAEDVPLLLM 

       250        260        270        280        290        300 
NPEAGKPSDL ILTRLLVPPL CIRPSVVSDL KSGTNEDDLT MKLTEIIFLN DVIKKHRISG 

       310        320        330        340        350        360 
AKTQMIMEDW DFLQLQCALY INSELSGIPL NMAPKKWTRG FVQRLKGKQG RFRGNLSGKR 

       370        380        390        400        410        420 
VDFSGRTVIS PDPNLRIDEV AVPVHVAKIL TFPEKVNKAN INFLRKLVQN GPEVHPGANF 

       430        440        450        460        470        480 
IQQRHTQMKR FLKYGNREKM AQELKYGDIV ERHLIDGDVV LFNRQPSLHK LSIMAHLARV 

       490        500        510        520        530        540 
KPHRTFRFNE CVCTPYNADF DGDEMNLHLP QTEEAKAEAL VLMGTKANLV TPRNGEPLIA 

       550        560        570        580        590        600 
AIQDFLTGAY LLTLKDTFFD RAKACQIIAS ILVGKDEKIK VRLPPPTILK PVTLWTGKQI 

       610        620        630        640        650        660 
FSVILRPSDD NPVRANLRTK GKQYCGKGED LCANDSYVTI QNSELMSGSM DKGTLGSGSK 

       670        680        690        700        710        720 
NNIFYILLRD WGQNEAADAM SRLARLAPVY LSNRGFSIGI GDVTPGQGLL KAKYELLNAG 

       730        740        750        760        770        780 
YKKCDEYIEA LNTGKLQQQP GCTAEETLEA LILKELSVIR DHAGSACLRE LDKSNSPLTM 

       790        800        810        820        830        840 
ALCGSKGSFI NISQMIACVG QQAISGSRVP DGFENRSLPH FEKHSKLPAA KGFVANSFYS 

       850        860        870        880        890        900 
GLTPTEFFFH TMAGREGLVD TAVKTAETGY MQRRLVKSLE DLCSQYDLTV RSSTGDIIQF 

       910        920        930        940        950        960 
IYGGDGLDPA AMEGKDEPLE FKRVLDNIKA VFPCPSEPAL SKNELILTTE SIMKKSEFLC 

       970        980        990       1000       1010       1020 
CQDSFLQEIK KFIKGVSEKI KKTRDKYGIN DNGTTEPRVL YQLDRITPTQ VEKFLETCRD 

      1030       1040       1050       1060       1070       1080 
KYMRAQMEPG SAVGALCAQS IGEPGTQMTL KTFHFAGVAS MNITLGVPRI KEIINASKAI 

      1090       1100       1110       1120       1130       1140 
STPIITAQLD KDDDADYARL VKGRIEKTLL GEISEYIEEV FLPDDCFILV KLSLERIRLL 

      1150       1160       1170       1180       1190       1200 
RLEVNAETVR YSICTSKLRV KPGDVAVHGE AVVCVTPREN SKSSMYYVLQ FLKEDLPKVV 

      1210       1220       1230       1240       1250       1260 
VQGIPEVSRA VIHIDEQSGK EKYKLLVEGD NLRAVMATHG VKGTRTTSNN TYEVEKTLGI 

      1270       1280       1290       1300       1310       1320 
EAARTTIINE IQYTMVNHGM SIDRRHVMLL SDLMTYKGEV LGITRFGLAK MKESVLMLAS 

      1330       1340       1350       1360       1370       1380 
FEKTADHLFD AAYFGQKDSV CGVSECIIMG IPMNIGTGLF KLLHKADRDP NPPKRPLIFD 

      1390 
TNEFHIPLVT 

O14802 in FASTA format

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