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UniProtKB/Swiss-Prot entry O14423


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name CBH1_SCHPO
Primary accession number O14423
Secondary accession number O14294
Integrated into Swiss-Prot on January 11, 2001
Sequence was last modified on October 1, 2000 (Sequence version 2)
Annotations were last modified on    May 5, 2009 (Entry version 69)
Name and origin of the protein
Protein name CENP-B homolog protein 1
Synonym CBHP-1
Gene name
Name: cbh1
Synonyms: cbh
ORFNames: SPAC9E9.10c
From
Schizosaccharomyces pombe (Fission yeast) [TaxID: 4896] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, AND FUNCTION.
STRAIN=ATCC 38366 / 972;
DOI=10.1073/pnas.94.16.8427; PubMed=9237993 [NCBI, ExPASy, EBI, Israel, Japan]
Lee J.-K., Huberman J.A., Hurwitz J.;
"Purification and characterization of a CENP-B homologue protein that binds to the centromeric K-type repeat DNA of Schizosaccharomyces pombe.";
Proc. Natl. Acad. Sci. U.S.A. 94:8427-8432(1997).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 38366 / 972;
DOI=10.1038/nature724; PubMed=11859360 [NCBI, ExPASy, EBI, Israel, Japan]
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M., Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.;
"The genome sequence of Schizosaccharomyces pombe.";
Nature 415:871-880(2002).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
U86754; AAB80698.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
CU329670; CAB16408.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR T39217; T39217.
RefSeq NP_594583.1; -.
3D structure databases
HSSP P49777; 1IUF. [HSSP ENTRY / PDB]
ModBase O14423.
Enzyme and pathway databases
BioCyc SPOM-XXX-01:SPOM-XXX-01-002679-MON; -.
Organism-specific databases
GeneDB_Spombe SPAC9E9.10c; -.
Gene expression databases
ArrayExpress O14423; -.
Ontologies
GO
GO:0000780; Cellular component: condensed nuclear chromosome, centromeric region (inferred from direct assay from GeneDB_SPombe).
GO:0000790; Cellular component: nuclear chromatin (non-traceable author statement from GeneDB_SPombe).
GO:0005819; Cellular component: spindle (inferred from direct assay from GeneDB_SPombe).
GO:0019237; Molecular function: centromeric DNA binding (inferred from direct assay from GeneDB_SPombe).
GO:0046983; Molecular function: protein dimerization activity (non-traceable author statement from GeneDB_SPombe).
GO:0030702; Biological process: chromatin silencing at centromere (inferred from mutant phenotype from GeneDB_SPombe).
GO:0007059; Biological process: chromosome segregation (inferred from genetic interaction from GeneDB_SPombe).
GO:0016570; Biological process: histone modification (inferred from mutant phenotype from GeneDB_SPombe).
QuickGo view.
Family and domain databases
InterPro IPR015175; Centromere_CenpB_DNA-db_2.
IPR006600; Centromere_CenpB_HTH.
IPR004875; DDE_SF_endonuclease_CENPB-like.
IPR012287; Homeodomain-rel.
Graphical view of domain structure.
Gene3D G3DSA:1.10.10.60; Homeodomain-rel; 2.
Pfam PF09091; CenpB-DNA-bind; 1.
PF03184; DDE; 1.
Pfam graphical view of domain structure.
SMART SM00674; CENPB; 1.
SMART graphical view of domain structure.
PROSITE PS51253; HTH_CENPB; 1.
PROSITE graphical view of domain structure (profiles).
Genome annotation databases
GeneID 2543290; -.
KEGG spo:SPAC9E9.10c; -.
NMPDR fig|4896.1.peg.4553; -.
Other
ProtoNet O14423.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Centromere; Complete proteome; Direct protein sequencing; DNA-binding; Nucleus.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   514  514     CENP-B homolog protein 1. PRO_0000126134
DOMAIN   69   144  76     HTH CENPB-type. 
COMPBIAS   421   493  73     Asp/Glu-rich (acidic). 
CONFLICT   168   168        I -> M (in Ref. 1; AAB80698). 
Sequence information
Length: 514 AA [This is the length of the unprocessed precursor] Molecular weight: 59865 Da [This is the MW of the unprocessed precursor] CRC64: A48102EBAD0BB1E3 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MPPIRQAVSL AEKKAIRDYY NQSAHRIPQK EVTEWFRKTY NKDLSQSTIS EILSPKYSYL 

        70         80         90        100        110        120 
DDGSVRLGDI KKIRAPKFPL LDNAVYEWLQ QREVEGLPIS GDMIKQAATR FWSKIPAYAS 

       130        140        150        160        170        180 
LPLPDFSNGW LDKFRRRHYI QQSAVNQALE SIKFEVFREY PVHIQEFIRP YAQKDIFCVG 

       190        200        210        220        230        240 
GTSLFWKLTP NKQNLDYQEI QGMKRGKAKV SLIMCCNADG SERLPLWIVG YAQNPRSFEQ 

       250        260        270        280        290        300 
CGIFPKSMNF EWKWNGRASI SPIIMEEWLR WFDTRMQGRK VLLMLESNDT YQFGVESVRR 

       310        320        330        340        350        360 
FKGGFQNTSV FRIPEKTLDI KSPFEQGIVD TFKANYRRYW LQYSLNQINI LRDPLKAVNV 

       370        380        390        400        410        420 
LKAIRWMLWS WNFGVSEKTI QASFLRSGLL GPHSEAEGQG MESYQKAIME IGNLISSKGS 

       430        440        450        460        470        480 
EAVKQINEYI RPSEEDETKL HQDAVDTVAA EFLEERRFES DEEEDVEPQI SNVEAAMAFE 

       490        500        510 
VLIKYFEQHE NGDPSFVLDL YRRQRVIAPN NLIA 

O14423 in FASTA format

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