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UniProtKB/Swiss-Prot entry O14104


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name LYS12_SCHPO
Primary accession number O14104
Secondary accession numbers None
Integrated into Swiss-Prot on November 13, 2007
Sequence was last modified on January 1, 1998 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 43)
Name and origin of the protein
Protein name Probable homoisocitrate dehydrogenase
Synonym EC 1.1.1.87
Gene name
Name: lys12
ORFNames: SPAC31G5.04
From
Schizosaccharomyces pombe (Fission yeast) [TaxID: 4896] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 38366 / 972;
DOI=10.1038/nature724; PubMed=11859360 [NCBI, ExPASy, EBI, Israel, Japan]
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M., Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.;
"The genome sequence of Schizosaccharomyces pombe.";
Nature 415:871-880(2002).
[2]
SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
DOI=10.1038/nbt1222; PubMed=16823372 [NCBI, ExPASy, EBI, Israel, Japan]
Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.;
"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.";
Nat. Biotechnol. 24:841-847(2006).
[3]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-81 AND SER-91, AND MASS SPECTROMETRY.
DOI=10.1021/pr7006335; PubMed=18257517 [NCBI, ExPASy, EBI, Israel, Japan]
Wilson-Grady J.T., Villen J., Gygi S.P.;
"Phosphoproteome analysis of fission yeast.";
J. Proteome Res. 7:1088-1097(2008).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
CU329670; CAB11688.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR T38621; T38621.
RefSeq NP_594004.1; -.
3D structure databases
HSSP P12010; 2AYQ. [HSSP ENTRY / PDB]
ModBase O14104.
Organism-specific databases
GeneDB_Spombe SPAC31G5.04; -.
Gene expression databases
ArrayExpress O14104; -.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from direct assay from GeneDB_SPombe).
QuickGo view.
Family and domain databases
InterPro IPR001804; IsoCit_IM_DHase.
Graphical view of domain structure.
Gene3D G3DSA:3.40.718.10; IDH_IMDH; 1.
PANTHER PTHR11835; IDH_IMDH_dimeric; 1.
Pfam PF00180; Iso_dh; 1.
Pfam graphical view of domain structure.
PROSITE PS00470; IDH_IMDH; 1.
BLOCKS O14104.
Genome annotation databases
GeneID 2542609; -.
KEGG spo:SPAC31G5.04; -.
NMPDR fig|4896.1.peg.3974; -.
Other
ProtoNet O14104.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Amino-acid biosynthesis; Complete proteome; Cytoplasm; Lysine biosynthesis; Magnesium; Manganese; Metal-binding; NAD; Oxidoreductase; Phosphoprotein.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   362  362     Probable homoisocitrate dehydrogenase. PRO_0000310374
METAL   232   232        Magnesium or manganese (By similarity). 
METAL   256   256        Magnesium or manganese (By similarity). 
METAL   260   260        Magnesium or manganese (By similarity). 
BINDING   97    97        Substrate (By similarity). 
BINDING   107   107        Substrate (By similarity). 
BINDING   126   126        Substrate (By similarity). 
BINDING   232   232        Substrate (By similarity). 
SITE   133   133  1     Critical for catalysis (By similarity). 
SITE   196   196  1     Critical for catalysis (By similarity). 
MOD_RES   81    81        Phosphoserine. 
MOD_RES   91    91        Phosphoserine. 
Sequence information
Length: 362 AA [This is the length of the unprocessed precursor] Molecular weight: 39256 Da [This is the MW of the unprocessed precursor] CRC64: 85865AFD69BCE773 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSATRRIVLG LIPADGIGKE VVPAARRLME NLPAKHKLKF DFIDLDAGWG TFERTGKALP 

        70         80         90        100        110        120 
ERTVERLKTE CNAALFGAVQ SPTHKVAGYS SPIVALRKKM GLYANVRPVK SLDGAKGKPV 

       130        140        150        160        170        180 
DLVIVRENTE CLYVKEERMV QNTPGKRVAE AIRRISEEAS TKIGKMAFEI AKSRQKIRES 

       190        200        210        220        230        240 
GTYSIHKKPL VTIIHKSNVM SVTDGLFRES CRHAQSLDPS YASINVDEQI VDSMVYRLFR 

       250        260        270        280        290        300 
EPECFDVVVA PNLYGDILSD GAASLIGSLG LVPSANVGDN FVMSEPVHGS APDIAGRGIA 

       310        320        330        340        350        360 
NPVATFRSVA LMLEFMGHQD AAADIYTAVD KVLTEGKVLT PDLGGKSGTN EITDAVLANI 


HN 

O14104 in FASTA format

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