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UniProtKB/Swiss-Prot entry O13911


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name PNK1_SCHPO
Primary accession number O13911
Secondary accession numbers None
Integrated into Swiss-Prot on July 15, 1998
Sequence was last modified on January 1, 1998 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 55)
Name and origin of the protein
Protein name Bifunctional polynucleotide phosphatase/kinase
Synonyms Polynucleotide kinase-3'-phosphatase
DNA 5'-kinase/3'-phosphatase
Includes Polynucleotide 3'-phosphatase
     (EC 3.1.3.32)
     (2'(3')-polynucleotidase)
Polynucleotide 5'-hydroxyl-kinase
     (EC 2.7.1.78)
Gene name
Name: pnk1
ORFNames: SPAC23C11.04c
From
Schizosaccharomyces pombe (Fission yeast) [TaxID: 4896] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND SUBCELLULAR LOCATION.
DOI=10.1074/jbc.M109383200; PubMed=11729194 [NCBI, ExPASy, EBI, Israel, Japan]
Meijer M., Karimi-Busheri F., Huang T.Y., Weinfeld M., Young D.;
"Pnk1, a DNA kinase/phosphatase required for normal response to DNA damage by gamma-radiation or camptothecin in Schizosaccharomyces pombe.";
J. Biol. Chem. 277:4050-4055(2002).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 38366 / 972;
DOI=10.1038/nature724; PubMed=11859360 [NCBI, ExPASy, EBI, Israel, Japan]
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M., Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.;
"The genome sequence of Schizosaccharomyces pombe.";
Nature 415:871-880(2002).
[3]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-8, AND MASS SPECTROMETRY.
DOI=10.1021/pr7006335; PubMed=18257517 [NCBI, ExPASy, EBI, Israel, Japan]
Wilson-Grady J.T., Villen J., Gygi S.P.;
"Phosphoproteome analysis of fission yeast.";
J. Proteome Res. 7:1088-1097(2008).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
CU329670; CAB11157.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR T38242; T38242.
RefSeq NP_593635.1; -.
3D structure databases
ModBase O13911.
Enzyme and pathway databases
BioCyc SPOM-XXX-01:SPOM-XXX-01-001304-MON; -.
Organism-specific databases
GeneDB_Spombe SPAC23C11.04c; -.
Gene expression databases
ArrayExpress O13911; -.
Ontologies
GO
GO:0005730; Cellular component: nucleolus (inferred from direct assay from GeneDB_SPombe).
GO:0005524; Molecular function: ATP binding (inferred from electronic annotation from UniProtKB-KW).
GO:0046404; Molecular function: ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity (inferred from electronic annotation from EC).
GO:0046403; Molecular function: polynucleotide 3'-phosphatase activity (inferred from electronic annotation from EC).
GO:0000718; Biological process: nucleotide-excision repair, DNA damage removal (inferred from direct assay from GeneDB_SPombe).
QuickGo view.
Family and domain databases
InterPro IPR006551; DNA-3-Pase.
IPR006549; HAD-SF_hydro_IIIA.
IPR013954; PNK3P_central-region.
IPR015636; PNK_3Pase.
Graphical view of domain structure.
PANTHER PTHR12083; PNK_3Pase; 1.
Pfam PF08645; PNK3P; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR01664; DNA-3_-Pase; 1.
TIGR01662; HAD-SF-IIIA; 1.
Genome annotation databases
GeneID 2542032; -.
KEGG spo:SPAC23C11.04c; -.
NMPDR fig|4896.1.peg.3605; -.
Other
ProtoNet O13911.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
ATP-binding; Complete proteome; DNA damage; DNA repair; Hydrolase; Kinase; Multifunctional enzyme; Nucleotide-binding; Nucleus; Phosphoprotein; Transferase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   421  421     Bifunctional polynucleotide phosphatase/kinase. PRO_0000058477
NP_BIND   263   270  8     ATP (Potential). 
MOD_RES   8     8        Phosphoserine. 
Sequence information
Length: 421 AA [This is the length of the unprocessed precursor] Molecular weight: 48477 Da [This is the MW of the unprocessed precursor] CRC64: FE9A732CD40E3146 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSSKKRKSPP QESLTSYFEK SSKSSKKYGS QNKDSDSSST CLQQKIEIQW SITDSLYIAK 

        70         80         90        100        110        120 
YGKLKKTKKF IAFDLDGTLI KTKSGRVFSK DAADWTWWHP SVVPKLKALY QDNYSLVIFS 

       130        140        150        160        170        180 
NQNGIPRKPS AGHTFQMKIR AIFESLDLPI VLYAAILKDK FRKPLTGMWN SFLKDVNRSI 

       190        200        210        220        230        240 
DLSFIKYVGD AAGRPGDHNS TDLKFAENIG IKFETPEQFF LGHSFVPPNF ESFHPKNYLV 

       250        260        270        280        290        300 
RNSSSHPYHF KKSEHQEIVV LVGFPSSGKS TLAESQIVTQ GYERVNQDIL KTKSKCIKAA 

       310        320        330        340        350        360 
IEALKKEKSV VIGMYSIIST TYAISDNTNP TIESRKMWID IAQEFEIPIR CIHLQSSEEL 

       370        380        390        400        410        420 
ARHNNVFRYI HHNQKQLPEI AFNSFKSRFQ MPTVEEGFTN VEEVPFKCLK DYEDTWNYWY 


E 

O13911 in FASTA format

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View entry in raw text format (no links)
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