RNA-directed RNA polymerase
     (EC 2.7.7.48)
mRNA (guanine-N(7)-)-methyltransferase
     (EC 2.1.1.56)
mRNA guanylyltransferase
     (EC 2.7.7.-)

Gene name

Name:

From

Rice yellow stunt virus (RYSV) (Rice transitory yellowing virus)

[TaxID: 59380]

Taxonomy

Viruses; ssRNA negative-strand viruses; Mononegavirales; Rhabdoviridae; Nucleorhabdovirus.

Virus host

Oryza sativa (Rice) [TaxID: 4530]

Protein existence

3: Inferred from homology;

References

[1]

NUCLEOTIDE SEQUENCE [GENOMIC RNA].
DOI=10.1099/vir.0.19195-0; PubMed=12867659 [NCBI, ExPASy, EBI, Israel, Japan]
Huang Y., Zhao H., Luo Z., Chen X., Fang R.X.;
"Novel structure of the genome of Rice yellow stunt virus: identification of the gene 6-encoded virion protein.";
J. Gen. Virol. 84:2259-2264(2003).

Comments

FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently five subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals (By similarity).
CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m₇G(5')pppR-RNA.
SUBUNIT: Interacts with the P protein (By similarity).
SUBCELLULAR LOCATION: Virion (Potential). Cytoplasm (By similarity).
SIMILARITY: Belongs to the rhabdoviridae protein L family.
SIMILARITY: Contains 1 RdRp catalytic domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AB011257; BAA25160.1; -; Genomic_RNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_620502.1; -.

3D structure databases

ModBase

O10378.

Ontologies

GO:0004482;	Molecular function: mRNA (guanine-N7-)-methyltransferase activity (inferred from electronic annotation from EC).
QuickGo view.

Family and domain databases

InterPro

IPR014023; RNA_pol_cat.
IPR001016; RNA_pol_L_viral.
Graphical view of domain structure.

Pfam

PF00946; Paramyx_RNA_pol; 1.
Pfam graphical view of domain structure.

PROSITE

PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

O10378.

Genome annotation databases

GeneID

944307; -.

Other

ProtoNet

O10378.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; Cytoplasm; Methyltransferase; mRNA capping; mRNA processing; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase; Virion.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 1967`	`1967`	`Large structural protein.`	`PRO_0000297839`
`DOMAIN`	`604 790`	`187`	`RdRp catalytic.`

Sequence information

Length: 1967 AA [This is the length of the unprocessed precursor]

Molecular weight: 223602 Da [This is the MW of the unprocessed precursor]

CRC64: DE70AB80A45FC776 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MDDEGHGYWQ DYDEDESWLD AENDVFDDDI FEEAEDNEHL VEGGDFHLKS ALRGEADMLT 

        70         80         90        100        110        120 
NPIYEKEREK LQEDVGDLGV ALGHLNVRPF LEKMGERIST DHTNTPLIEN IKQGGFGAIH 

       130        140        150        160        170        180 
HMKSTARLIV AEAATVTETM LTSGVDTTLA GAYHFFEQNH PHMKCTVNMV LMFLTILNNV 

       190        200        210        220        230        240 
KNIRLNVDLH QILKNNISIK GSTVCMYITV ATVAYFSTDI VVFDIDGQKY NTPKTYFLNA 

       250        260        270        280        290        300 
CDKIQERFNV ILYSYLAEGL SIPGSPPTYI VNRIIDWGDS ILYQMGNDGY DVIALYEATV 

       310        320        330        340        350        360 
VGVILSRDDK DLSPTSGGGD FLENIQQDLG PVQQNHIRYL IALLHDMTPH QLADLHGLYR 

       370        380        390        400        410        420 
IWAHPIIDID GGVKKLQKVT QSLKGDINSK PESQETVRSF RRLFVMDYFV KHQFYPPITL 

       430        440        450        460        470        480 
PEKSNCYIGN CIRTSKKIDE SHINYNFSDW DLVELGGAFS IPYSWNVLHL AKDKAISPTR 

       490        500        510        520        530        540 
SEMYTMLCKT KRVFNAELRR GVLKLMNTTL TPLREFLTEV AEHGLDIDDC IIGLFPKERE 

       550        560        570        580        590        600 
LKIMARFFAL LSFKMRLYFT ATEELLGSKL LRYFPQITMS SNLLDMQEKM SSMSRDLESQ 

       610        620        630        640        650        660 
NKSVTYVINM DFVKWNQQMR ESTCEGFLKN WSKLFGLPGL YSRSHQIFRD SILYIADGTR 

       670        680        690        700        710        720 
DLTPDPDTGI MVDNNVCWID DGAGKEGIRQ KAWTIMTVCD IAAVARHHPG VFHLVGGGDN 

       730        740        750        760        770        780 
QVLTVTYHTN QIDTDGNITE EGKSKIKAKV KKFIEALETH FAARGLPLKT SETWCSTSLF 

       790        800        810        820        830        840 
MYNKFMYYKG VPLRSPLKQV SRLFPYSNNT SMTLQSMAQC LGTGLRSAAQ KEVSHIALLF 

       850        860        870        880        890        900 
MRNIWGSVLG WIILYCHPML PSISSETCNS GVSTIVRGRK AINMKTHRID LVALILKILY 

       910        920        930        940        950        960 
LPGQLGGPGL VNIYQMTMRG FPDPVTEAIC FLRKFKNYLI CVGSSYSSHL ARMAGVSFSA 

       970        980        990       1000       1010       1020 
SRSYEPLIED VCSLNLDTPR SGTGGKREVP RKILLKSRLG GNQHLKELLG IMKGPSEGEF 

      1030       1040       1050       1060       1070       1080 
YRAISSGKVL DVRVMHEITS STLYAVTNTF TSRVDKTATL KRLTFKFSML ESLADAELKY 

      1090       1100       1110       1120       1130       1140 
IRYLSVRDDK THDIMFDGCS RIIADECRTK GWGKPVLGVT VPTPFEYLQI SWTDEHICDN 

      1150       1160       1170       1180       1190       1200 
NHITVRISNQ DRQVTTETLG PCKPYLGAYT KEKFKMTEVA AAYGDEDVLS KSLRILKIIN 

      1210       1220       1230       1240       1250       1260 
WRYQDGSTMS EIIKAPFRAV TDIDPQRMVQ ESTVTKGDYD HRRKMDARVH GGIPNFVTTP 

      1270       1280       1290       1300       1310       1320 
LSHISISTST WYKHARGKNE NIHFQACIIQ TMYQIIIRTM SNIHSQEKLH VHECCQTSIS 

      1330       1340       1350       1360       1370       1380 
EIQEPRANLD LTTPQMIPTF PTLMGNPLVY IPEQSITFDY SRTQEVDYSR RVGSLDCQRS 

      1390       1400       1410       1420       1430       1440 
TDYGWVDAYS SLSWLIMCDV IGWTKMPDSF YIMQQERVDH YLLSMYIISI WKVLKSEFDL 

      1450       1460       1470       1480       1490       1500 
HKTMLDWGPL LKVYSSDTGV QVLSQSMGIV VLGKLEGGLS VNMMDICNSL TGLTSNQIPP 

      1510       1520       1530       1540       1550       1560 
FSVNLALPRI HKQVATWWKL TCSDTMYCIQ CSNILKGYWE GTLINTRIDN DLRCSECADG 

      1570       1580       1590       1600       1610       1620 
GISPRIVNAH ISNLSDHLIK IIPDREEVQL PLLAGGEDLN NLEVVLSLED EWPDSDDITI 

      1630       1640       1650       1660       1670       1680 
LNRIGEMNDI DGRMKNYLEV MALLNPDVVI IRPEILELEI LRRVCEVVRL ASEGKLIIHI 

      1690       1700       1710       1720       1730       1740 
LVEEPSYLES GAIYEMERAF PRHNLFHVQF SFRKPPEGLH KLWLLPQDIT LARADVGDWL 

      1750       1760       1770       1780       1790       1800 
VIPFTQLLDF HDTITPDTRL HHQREAIRQD VFWSTKEQGG LGLVVLQHIC TKTELSYRLD 

      1810       1820       1830       1840       1850       1860 
RSKVDVEINK MMIKDPVMGG KYVILKRRCS WAIFSTKYEL LCSAERIKGN LSADGKKITS 

      1870       1880       1890       1900       1910       1920 
KDFSRTYKED IIIFIISVLM RSLDCDEKRV MTLSYVECLP ELLTLKPRFN KSGVISVRYL 

      1930       1940       1950       1960 
DYFWFFKRVY SYNNPEVAIP YSAVIKGVNL GSPRKEGYSL ASSDGAP

O10378 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools