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UniProtKB/Swiss-Prot entry O00461


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name GOLI4_HUMAN
Primary accession number O00461
Secondary accession numbers None
Integrated into Swiss-Prot on May 1, 2007
Sequence was last modified on July 1, 1997 (Sequence version 1)
Annotations were last modified on    June 16, 2009 (Entry version 52)
Name and origin of the protein
Protein name Golgi integral membrane protein 4
Synonyms Golgi phosphoprotein 4
Golgi integral membrane protein, cis
GIMPc
Golgi-localized phosphoprotein of 130 kDa
Golgi phosphoprotein of 130 kDa
Gene name
Name: GOLIM4
Synonyms: GIMPC, GOLPH4, GPP130
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, PHOSPHORYLATION, GLYCOSYLATION, AND TOPOLOGY.
TISSUE=Small intestine;
PubMed=9201717 [NCBI, ExPASy, EBI, Israel, Japan]
Linstedt A.D., Mehta A., Suhan J., Reggio H., Hauri H.-P.;
"Sequence and overexpression of GPP130/GIMPc: evidence for saturable pH-sensitive targeting of a type II early Golgi membrane protein.";
Mol. Biol. Cell 8:1073-1087(1997).
[2]
SUBCELLULAR LOCATION.
PubMed=11598199 [NCBI, ExPASy, EBI, Israel, Japan]
Bachert C., Lee T.H., Linstedt A.D.;
"Lumenal endosomal and Golgi-retrieval determinants involved in pH-sensitive targeting of an early Golgi protein.";
Mol. Biol. Cell 12:3152-3160(2001).
[3]
FUNCTION.
DOI=10.1091/mbc.E04-05-0366; PubMed=15331763 [NCBI, ExPASy, EBI, Israel, Japan]
Natarajan R., Linstedt A.D.;
"A cycling cis-Golgi protein mediates endosome-to-Golgi traffic.";
Mol. Biol. Cell 15:4798-4806(2004).
[4]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-394 AND SER-396, AND MASS SPECTROMETRY.
TISSUE=Epithelium;
DOI=10.1016/j.cell.2006.09.026; PubMed=17081983 [NCBI, ExPASy, EBI, Israel, Japan]
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.;
"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.";
Cell 127:635-648(2006).
[5]
IDENTIFICATION [LARGE SCALE ANALYSIS], AND MASS SPECTROMETRY.
Colinge J., Superti-Furga G., Bennett K.L.;
Submitted (OCT-2008) to UniProtKB.
[6]
VARIANT [LARGE SCALE ANALYSIS] VAL-312.
DOI=10.1126/science.1133427; PubMed=16959974 [NCBI, ExPASy, EBI, Israel, Japan]
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V., Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.;
"The consensus coding sequences of human breast and colorectal cancers.";
Science 314:268-274(2006).
Comments
  • FUNCTION: Plays a role in endosome to Golgi protein trafficking; mediates protein transport along the late endosome-bypass pathway from the early endosome to the Golgi.
  • SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Endosome membrane; Single-pass type II membrane protein. Note=Localizes to cis and medial Golgi cisternae. Probably cycles between early Golgi and distal compartments like endosome.
  • PTM: Phosphorylated probably by c-AMP-dependent kinases in its lumenal part.
  • PTM: O-glycosylated; modified by sialic acid residues (By similarity).
  • PTM: N-glycosylated; N-glycans are probably of the complex type and modified by sialic acid residues.
  • SIMILARITY: Belongs to the GOLIM4 family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
U55853; AAB58419.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
IPI IPI00004962; -.
RefSeq NP_055313.1; -.
UniGene Hs.143600
3D structure databases
ModBase O00461.
PTM databases
PhosphoSite O00461; -.
Organism-specific databases
GeneCards GC03M169210; -.
HGNC HGNC:15448; GOLIM4.
GenAtlas GOLIM4.
HPA HPA001677; -.
HPA002315; -.
MIM 606805; gene. [NCBI / EBI]
PharmGKB PA28813; -.
Gene expression databases
ArrayExpress O00461; -.
Bgee O00461; -.
CleanEx HS_GOLIM4; -.
Ontologies
GO
GO:0005801; Cellular component: cis-Golgi network (traceable author statement from ProtInc).
GO:0030139; Cellular component: endocytic vesicle (traceable author statement from ProtInc).
GO:0010008; Cellular component: endosome membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0032580; Cellular component: Golgi cisterna membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0005796; Cellular component: Golgi lumen (traceable author statement from ProtInc).
GO:0016021; Cellular component: integral to membrane (traceable author statement from ProtInc).
GO:0005634; Cellular component: nucleus (inferred from direct assay from HPA).
GO:0006810; Biological process: transport (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Proteomic databases
PeptideAtlas O00461; -.
PRIDE O00461; -.
Genome annotation databases
Ensembl ENSG00000173905; Homo sapiens. [Contig view]
GeneID 27333; -.
KEGG hsa:27333; -.
Phylogenomic databases
HOVERGEN O00461; -.
OMA O00461; QDSNSRY.
Other
NextBio 50382; -.
SOURCE GOLIM4; Homo sapiens.
ProtoNet O00461.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Coiled coil; Endosome; Glycoprotein; Golgi apparatus; Membrane; Phosphoprotein; Polymorphism; Signal-anchor; Transmembrane; Transport.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   696  696     Golgi integral membrane protein 4. PRO_0000285097
TOPO_DOM   1    12  12     Cytoplasmic (Potential). 
TRANSMEM   13    33  21     Signal-anchor for type II membrane protein (Potential). 
TOPO_DOM   34   696  663     Lumenal (Potential). 
REGION   38   107  70     Golgi targeting. 
REGION   80   175  96     Endosome targeting. 
REGION   176   248  73     Golgi targeting. 
COILED   35   244  210     Potential. 
COMPBIAS   311   681  371     Glu-rich. 
COMPBIAS   404   513  110     Gln-rich. 
MOD_RES   394   394        Phosphotyrosine. 
MOD_RES   396   396        Phosphoserine. 
MOD_RES   673   673        Phosphotyrosine (By similarity). 
CARBOHYD   257   257        N-linked (GlcNAc...) (Potential). 
VARIANT   312   312  1     A -> V (in a breast cancer sample; somatic mutation). VAR_036611 
Sequence information
Length: 696 AA [This is the length of the unprocessed precursor] Molecular weight: 81880 Da [This is the MW of the unprocessed precursor] CRC64: A3BA9AE3D52F7174 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MGNGMCSRKQ KRIFQTLLLL TVVFGFLYGA MLYYELQTQL RKAEAVALKY QQHQESLSAQ 

        70         80         90        100        110        120 
LQVVYEHRSR LEKSLQKERL EHKKAKEDFL VYKLEAQETL NKGRQDSNSR YSALNVQHQM 

       130        140        150        160        170        180 
LKSQHEELKK QHSDLEEEHR KQGEDFSRTF NDHKQKYLQL QQEKEQELSK LKETVYNLRE 

       190        200        210        220        230        240 
ENRQLRKAHQ DIHTQLQDVK QQHKNLLSEH EQLVVTLEDH KSALAAAQTQ VAEYKQLKDT 

       250        260        270        280        290        300 
LNRIPSLRKP DPAEQQNVTQ VAHSPQGYNT AREKPTREVQ EVSRNNDVWQ NHEAVPGRAE 

       310        320        330        340        350        360 
DTKLYAPTHK EAEFQAPPEP IQQEVERREP EEHQVEEEHR KALEEEEMEQ VGQAEHLEEE 

       370        380        390        400        410        420 
HDPSPEEQDR EWKEQHEQRE AANLLEGHAR AEVYPSAKPM IKFQSPYEEQ LEQQRLAVQQ 

       430        440        450        460        470        480 
VEEAQQLREH QEALHQQRLQ GHLLRQQEQQ QQQVAREMAL QRQAELEEGR PQHQEQLRQQ 

       490        500        510        520        530        540 
AHYDAMDNDI VQGAEDQGIQ GEEGAYERDN QHQDEAEGDP GNRHEPREQG PREADPESEA 

       550        560        570        580        590        600 
DRAAVEDINP ADDPNNQGED EFEEAEQVRE ENLPDENEEQ KQSNQKQENT EVEEHLVMAG 

       610        620        630        640        650        660 
NPDQQEDNVD EQYQEEAEEE VQEDLTEEKK RELEHNAEET YGENDENTDD KNNDGEEQEV 

       670        680        690 
RDDNRPKGRE EHYEEEEEEE EDGAAVAEKS HRRAEM 

O00461 in FASTA format

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