ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!
Search for

UniProtKB/Swiss-Prot entry O00459


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

Note: most headings are clickable, even if they don't appear as links. They link to the user manual or other documents.
Entry information
Entry name P85B_HUMAN
Primary accession number O00459
Secondary accession numbers Q5EAT5 Q9UPH9
Integrated into Swiss-Prot on December 15, 1998
Sequence was last modified on July 1, 1997 (Sequence version 1)
Annotations were last modified on    December 16, 2008 (Entry version 88)
Name and origin of the protein
Protein name Phosphatidylinositol 3-kinase regulatory subunit beta
Synonyms PI3-kinase p85 subunit beta
PtdIns-3-kinase p85-beta
Gene name
Name: PIK3R2
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
DOI=10.1038/sj.onc.1201695; PubMed=9582025 [NCBI, ExPASy, EBI, Israel, Japan]
Janssen J.W.G., Schleithhoff L., Bartram C.R., Schulz A.S.;
"An oncogenic fusion product of the phosphatidylinositol 3-kinase p85beta subunit and HUMORF8, a putative deubiquitinating enzyme.";
Oncogene 16:1767-1772(1998).
[2]
NUCLEOTIDE SEQUENCE.
PubMed=1314371 [NCBI, ExPASy, EBI, Israel, Japan]
Volinia S., Patracchini P., Otsu M., Hiles I., Gout I., Calzolari E., Bernardi F., Rooke L., Waterfield M.D.;
"Chromosomal localization of human p85 alpha, a subunit of phosphatidylinositol 3-kinase, and its homologue p85 beta.";
Oncogene 7:789-793(1992).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANTS SER-234 AND SER-313.
DOI=10.1038/nature02399; PubMed=15057824 [NCBI, ExPASy, EBI, Israel, Japan]
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V., Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.;
"The DNA sequence and biology of human chromosome 19.";
Nature 428:529-535(2004).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Brain, and Placenta;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[5]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-464, AND MASS SPECTROMETRY.
TISSUE=Epithelium;
DOI=10.1074/mcp.M500089-MCP200; PubMed=15951569 [NCBI, ExPASy, EBI, Israel, Japan]
Zhang Y., Wolf-Yadlin A., Ross P.L., Pappin D.J., Rush J., Lauffenburger D.A., White F.M.;
"Time-resolved mass spectrometry of tyrosine phosphorylation sites in the epidermal growth factor receptor signaling network reveals dynamic modules.";
Mol. Cell. Proteomics 4:1240-1250(2005).
[6]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-464, AND MASS SPECTROMETRY.
DOI=10.1038/nbt1046; PubMed=15592455 [NCBI, ExPASy, EBI, Israel, Japan]
Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.;
"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells.";
Nat. Biotechnol. 23:94-101(2005).
[7]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-365; TYR-464 AND TYR-467, AND MASS SPECTROMETRY.
DOI=10.1016/j.cell.2007.11.025; PubMed=18083107 [NCBI, ExPASy, EBI, Israel, Japan]
Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J., Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L., Mitchell J., Wetzel R., Macneill J., Ren J.M., Yuan J., Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D., Zhou X., Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J.;
"Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer.";
Cell 131:1190-1203(2007).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X80907; CAA56868.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AC007192; AAD22671.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC070082; AAH70082.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC090249; AAH90249.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR H59435; H59435.
RefSeq NP_005018.1; -.
UniGene Hs.371344
3D structure databases
HSSP P23727; 2PNB. [HSSP ENTRY / PDB]
SMR O00459; 324-436, 614-718.
ModBase O00459.
Protein-protein interaction databases
IntAct O00459; 3.
PTM databases
PhosphoSite O00459; -.
Enzyme and pathway databases
Reactome REACT_11044; Signaling by Rho GTPases.
REACT_498; Signaling by insulin receptor.
Organism-specific databases
GeneCards GC19P018089; -.
H-InvDB HIX0014905; -.
HGNC HGNC:8980; PIK3R2.
GenAtlas PIK3R2.
MIM 603157; gene. [NCBI / EBI]
PharmGKB PA33313; -.
GeneCards O00459.
Gene expression databases
ArrayExpress O00459; -.
CleanEx HS_PIK3R2; -.
GermOnline ENSG00000105647; Homo sapiens.
Ontologies
GO
GO:0005942; Cellular component: phosphoinositide 3-kinase complex (inferred from electronic annotation from InterPro).
GO:0035014; Molecular function: phosphoinositide 3-kinase regulator activity (inferred from electronic annotation from InterPro).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0019987; Biological process: negative regulation of anti-apoptosis (inferred from sequence or structural similarity from UniProtKB).
GO:0007165; Biological process: signal transduction (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR001720; PI3kinase_P85.
IPR000198; RhoGAP.
IPR000980; SH2.
IPR001452; SH3.
Graphical view of domain structure.
Gene3D G3DSA:1.10.555.10; RhoGAP; 1.
G3DSA:3.30.505.10; SH2; 2.
PANTHER PTHR10155; PI3kinase_P85; 1.
Pfam PF00620; RhoGAP; 1.
PF00017; SH2; 2.
PF00018; SH3_1; 1.
Pfam graphical view of domain structure.
PRINTS PR00678; PI3KINASEP85.
PR00401; SH2DOMAIN.
PR00452; SH3DOMAIN.
ProDom PD000093; SH2; 2.
PD000066; SH3; 1.
[Domain structure / List of seq. sharing at least 1 domain]
SMART SM00324; RhoGAP; 1.
SM00252; SH2; 2.
SM00326; SH3; 1.
SMART graphical view of domain structure.
PROSITE PS50238; RHOGAP; 1.
PS50001; SH2; 2.
PS50002; SH3; 1.
PROSITE graphical view of domain structure (profiles).
Proteomics databases
PRIDE O00459; -.
Genome annotation databases
Ensembl ENSG00000105647; Homo sapiens. [Contig view]
GeneID 5296; -.
KEGG hsa:5296; -.
Phylogenomic databases
HOGENOM O00459; -.
HOVERGEN O00459; -.
Other
NextBio 20470; -.
SOURCE PIK3R2; Homo sapiens.
ProtoNet O00459.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Phosphoprotein; Polymorphism; Repeat; SH2 domain; SH3 domain.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   728  728     Phosphatidylinositol 3-kinase regulatory subunit beta. PRO_0000080763
DOMAIN   4    80  77     SH3. 
DOMAIN   109   295  187     Rho-GAP. 
DOMAIN   330   425  96     SH2 1. 
DOMAIN   622   716  95     SH2 2. 
MOD_RES   365   365        Phosphotyrosine. 
MOD_RES   464   464        Phosphotyrosine. 
MOD_RES   467   467        Phosphotyrosine. 
VARIANT   234   234  1     R -> S (in dbSNP:rs2241088 [NCBI]). VAR_030679 
VARIANT   313   313  1     P -> S (in dbSNP:rs1011320 [NCBI]). VAR_030680 
Sequence information
Length: 728 AA [This is the length of the unprocessed precursor] Molecular weight: 81624 Da [This is the MW of the unprocessed precursor] CRC64: 80C2AF244977346B [This is a checksum on the sequence]
        10         20         30         40         50         60 
MAGPEGFQYR ALYPFRRERP EDLELLPGDV LVVSRAALQA LGVAEGGERC PQSVGWMPGL 

        70         80         90        100        110        120 
NERTRQRGDF PGTYVEFLGP VALARPGPRP RGPRPLPARP RDGAPEPGLT LPDLPEQFSP 

       130        140        150        160        170        180 
PDVAPPLLVK LVEAIERTGL DSESHYRPEL PAPRTDWSLS DVDQWDTAAL ADGIKSFLLA 

       190        200        210        220        230        240 
LPAPLVTPEA SAEARRALRE AAGPVGPALE PPTLPLHRAL TLRFLLQHLG RVARRAPALG 

       250        260        270        280        290        300 
PAVRALGATF GPLLLRAPPP PSSPPPGGAP DGSEPSPDFP ALLVEKLLQE HLEEQEVAPP 

       310        320        330        340        350        360 
ALPPKPPKAK PAPTVLANGG SPPSLQDAEW YWGDISREEV NEKLRDTPDG TFLVRDASSK 

       370        380        390        400        410        420 
IQGEYTLTLR KGGNNKLIKV FHRDGHYGFS EPLTFCSVVD LINHYRHESL AQYNAKLDTR 

       430        440        450        460        470        480 
LLYPVSKYQQ DQIVKEDSVE AVGAQLKVYH QQYQDKSREY DQLYEEYTRT SQELQMKRTA 

       490        500        510        520        530        540 
IEAFNETIKI FEEQGQTQEK CSKEYLERFR REGNEKEMQR ILLNSERLKS RIAEIHESRT 

       550        560        570        580        590        600 
KLEQQLRAQA SDNREIDKRM NSLKPDLMQL RKIRDQYLVW LTQKGARQKK INEWLGIKNE 

       610        620        630        640        650        660 
TEDQYALMED EDDLPHHEER TWYVGKINRT QAEEMLSGKR DGTFLIRESS QRGCYACSVV 

       670        680        690        700        710        720 
VDGDTKHCVI YRTATGFGFA EPYNLYGSLK ELVLHYQHAS LVQHNDALTV TLAHPVRAPG 


PGPPPAAR 

O00459 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

BLAST logo BLAST submission on ExPASy/SIB
or at NCBI (USA)
Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
PROSITE logo ScanProsite, MotifScan SWISS-MODEL Submit a homology modeling request to SWISS-MODEL
NPSA logo NPSA Sequence analysis tools

ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
 Hosted by ca flag CBR Canada Mirror sites: Australia  Brazil  China  Korea  Switzerland
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!