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UniProtKB/Swiss-Prot entry O00253


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name AGRP_HUMAN
Primary accession number O00253
Secondary accession numbers O15459 Q2TBD9
Integrated into Swiss-Prot on November 1, 1997
Sequence was last modified on July 1, 1997 (Sequence version 1)
Annotations were last modified on    June 16, 2009 (Entry version 84)
Name and origin of the protein
Protein name Agouti-related protein [Precursor]
Synonyms None
Gene name
Name: AGRP
Synonyms: AGRT, ART
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
PubMed=9119224 [NCBI, ExPASy, EBI, Israel, Japan]
Shutter J.R., Graham M., Kinsey A.C., Scully S., Luethy R., Stark K.L.;
"Hypothalamic expression of ART, a novel gene related to agouti, is up-regulated in obese and diabetic mutant mice.";
Genes Dev. 11:593-602(1997).
[2]
NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Adrenal gland;
DOI=10.1126/science.278.5335.135; PubMed=9311920 [NCBI, ExPASy, EBI, Israel, Japan]
Ollmann M.M., Wilson B.D., Yang Y.K., Kerns J.A., Chen Y., Gantz I., Barsh G.S.;
"Antagonism of central melanocortin receptors in vitro and in vivo by agouti-related protein.";
Science 278:135-138(1997).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT THR-67.
DOI=10.1016/S0378-1119(01)00705-3; PubMed=11602360 [NCBI, ExPASy, EBI, Israel, Japan]
Brown A.M., Mayfield D.K., Volaufova J., Argyropoulos G.;
"The gene structure and minimal promoter of the human agouti related protein.";
Gene 277:231-238(2001).
[4]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1038/sj.mp.4000854; PubMed=11326303 [NCBI, ExPASy, EBI, Israel, Japan]
Vink T., Hinney A., van Elburg A.A., van Goozen S.H., Sandkuijl L.A., Sinke R.J., Herpertz-Dahlmann B.M., Hebebrand J., Remschmidt H., van Engeland H., Adan R.A.;
"Association between an agouti-related protein gene polymorphism and anorexia nervosa.";
Mol. Psychiatry 6:325-328(2001).
[5]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
SeattleSNPs variation discovery resource;
Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
[6]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[7]
DISULFIDE BONDS.
DOI=10.1021/bi981082v; PubMed=9724530 [NCBI, ExPASy, EBI, Israel, Japan]
Bures E.J., Hui J.O., Young Y., Chow D.T., Katta V., Rohde M.F., Zeni L., Rosenfeld R.D., Stark K.L., Haniu M.;
"Determination of disulfide structure in agouti-related protein (AGRP) by stepwise reduction and alkylation.";
Biochemistry 37:12172-12177(1998).
[8]
STRUCTURE BY NMR OF 87-132.
DOI=10.1016/S0014-5793(99)00553-0; PubMed=10371151 [NCBI, ExPASy, EBI, Israel, Japan]
Bolin K.A., Anderson D.J., Trulson J.A., Thompson D.A., Wilken J., Kent S.B.H., Gantz I., Millhauser G.L.;
"NMR structure of a minimized human agouti related protein prepared by total chemical synthesis.";
FEBS Lett. 451:125-131(1999).
[9]
STRUCTURE BY NMR OF 87-120.
DOI=10.1021/bi012000x; PubMed=12056887 [NCBI, ExPASy, EBI, Israel, Japan]
Jackson P.J., McNulty J.C., Yang Y.K., Thompson D.A., Chai B., Gantz I., Barsh G.S., Millhauser G.L.;
"Design, pharmacology, and NMR structure of a minimized cystine knot with agouti-related protein activity.";
Biochemistry 41:7565-7572(2002).
[10]
VARIANT THR-67.
DOI=10.1210/jc.2002-011834; PubMed=12213871 [NCBI, ExPASy, EBI, Israel, Japan]
Argyropoulos G., Rankinen T., Neufeld D.R., Rice T., Province M.A., Leon A.S., Skinner J.S., Wilmore J.H., Rao D.C., Bouchard C.;
"A polymorphism in the human agouti-related protein is associated with late-onset obesity.";
J. Clin. Endocrinol. Metab. 87:4198-4202(2002).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
U88063; AAB52240.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U89485; AAB68621.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF314194; AAL09457.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF281309; AAK96256.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
DQ374394; ABC88473.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC110443; AAI10444.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
IPI IPI00010869; -.
RefSeq NP_001129.1; -.
NP_015531.1; -.
UniGene Hs.104633
3D structure databases
PDB
1HYK; NMR; -; A=87-132.[ExPASy / RCSB / EBI]
1MR0; NMR; -; A=87-120.[ExPASy / RCSB / EBI]
2IQV; Model; -; B=87-132.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1HYK; -.
1MR0; -.
2IQV; -.
ModBase O00253.
Enzyme and pathway databases
Pathway_Interaction_DB syndecan_3_pathway; Syndecan-3-mediated signaling events.
Organism-specific databases
GeneCards GC16M066073; -.
H-InvDB HIX0038652; -.
HGNC HGNC:330; AGRP.
GenAtlas AGRP.
MIM 601665; phenotype. [NCBI / EBI]
602311; gene. [NCBI / EBI]
PharmGKB PA24627; -.
Gene expression databases
ArrayExpress O00253; -.
Bgee O00253; -.
CleanEx HS_AGRP; -.
GermOnline ENSG00000159723; Homo sapiens.
Ontologies
GO
GO:0005576; Cellular component: extracellular region (inferred from electronic annotation from UniProtKB-SubCell).
GO:0005184; Molecular function: neuropeptide hormone activity (traceable author statement from ProtInc).
GO:0009755; Biological process: hormone-mediated signaling (inferred from electronic annotation from InterPro).
GO:0007218; Biological process: neuropeptide signaling pathway (traceable author statement from ProtInc).
QuickGo view.
Family and domain databases
InterPro IPR007733; Agouti.
Graphical view of domain structure.
Gene3D G3DSA:4.10.760.10; Agouti; 1.
PANTHER PTHR16551; Agouti; 1.
Pfam PF05039; Agouti; 1.
Pfam graphical view of domain structure.
SMART SM00792; Agouti; 1.
SMART graphical view of domain structure.
PROSITE PS60024; AGOUTI_1; 1.
PS51150; AGOUTI_2; 1.
PROSITE graphical view of domain structure (profiles).
Proteomic databases
PRIDE O00253; -.
Genome annotation databases
Ensembl ENSG00000159723; Homo sapiens. [Contig view]
GeneID 181; -.
KEGG hsa:181; -.
Phylogenomic databases
HOGENOM O00253; -.
HOVERGEN O00253; -.
OMA O00253; LAPLEGI.
Other
NextBio 738; -.
SOURCE AGRP; Homo sapiens.
ProtoNet O00253.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Disease mutation; Disulfide bond; Knottin; Obesity; Secreted; Signal.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
SIGNAL   1    20  20     Potential. 
CHAIN   21   132  112     Agouti-related protein. PRO_0000001034
DOMAIN   87   129  43     Agouti. 
DISULFID   87   102         
DISULFID   94   108         
DISULFID   101   119         
DISULFID   105   129         
DISULFID   110   117         
VARIANT   67    67  1     A -> T (in obesity; late onset; dbSNP:rs5030980 [NCBI]). VAR_015385 
CONFLICT   6     6        V -> L (in Ref. 2; AAB68621). 
STRAND   95    97  3      
STRAND   101   103  3      
STRAND   107   115  9      
STRAND   117   120  4      
STRAND   125   127  3      
Sequence information
Length: 132 AA [This is the length of the unprocessed precursor] Molecular weight: 14440 Da [This is the MW of the unprocessed precursor] CRC64: 1CCBE112C3EB10F5 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MLTAAVLSCA LLLALPATRG AQMGLAPMEG IRRPDQALLP ELPGLGLRAP LKKTTAEQAE 

        70         80         90        100        110        120 
EDLLQEAQAL AEVLDLQDRE PRSSRRCVRL HESCLGQQVP CCDPCATCYC RFFNAFCYCR 

       130 
KLGTAMNPCS RT 

O00253 in FASTA format

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