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UniProtKB/Swiss-Prot entry O00222


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name GRM8_HUMAN
Primary accession number O00222
Secondary accession numbers A4D0Y3 B0FZ74 B0M0L0 O15493 O95945 O95946 Q3MIV9 Q52M02 Q6J165
Integrated into Swiss-Prot on November 1, 1997
Sequence was last modified on December 1, 2000 (Sequence version 2)
Annotations were last modified on    June 16, 2009 (Entry version 89)
Name and origin of the protein
Protein name Metabotropic glutamate receptor 8 [Precursor]
Synonym mGluR8
Gene name
Name: GRM8
Synonyms: GPRC1H, MGLUR8
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 2: Evidence at transcript level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
DOI=10.1006/geno.1997.4842; PubMed=9299241 [NCBI, ExPASy, EBI, Israel, Japan]
Scherer S.W., Soder S., Duvoisin R.M., Huizenga J.J., Tsui L.-C.;
"The human metabotropic glutamate receptor 8 (GRM8) gene: a disproportionately large gene located at 7q31.3-q32.1.";
Genomics 44:232-236(1997).
[2]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), AND VARIANT ASN-768.
DOI=10.1016/S0169-328X(97)00277-5; PubMed=9473604 [NCBI, ExPASy, EBI, Israel, Japan]
Wu S., Wright R.A., Rockey P.K., Burgett S.G., Arnold J.S., Rosteck P.R. Jr., Johnson B.G., Schoepp D.D., Belagaje R.M.;
"Group III human metabotropic glutamate receptors 4, 7 and 8: molecular cloning, functional expression, and comparison of pharmacological properties in RGT cells.";
Brain Res. Mol. Brain Res. 53:88-97(1998).
[3]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS B AND C).
TISSUE=Fetal brain;
DOI=10.1016/S0169-328X(99)00050-9; PubMed=10216218 [NCBI, ExPASy, EBI, Israel, Japan]
Malherbe P., Kratzeisen C., Lundstrom K., Richards J.G., Faull R.L.M., Mutel V.;
"Cloning and functional expression of alternative spliced variants of the human metabotropic glutamate receptor 8.";
Brain Res. Mol. Brain Res. 67:201-210(1999).
[4]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
Stormann T.M., Simin R.T., Hammerland L.G., Fuller F.H.;
"Homo sapiens metabotropic glutamate receptor.";
Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
[5]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
TISSUE=Brain;
Kaighin V.A., Martin A.L., Aronstam R.S.;
Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases.
[6]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS CYS-10; THR-265; TYR-362; ASP-368 AND PHE-430.
NIEHS SNPs program;
Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases.
[7]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORM A), AND VARIANT GLN-343.
DOI=10.1038/nature01782; PubMed=12853948 [NCBI, ExPASy, EBI, Israel, Japan]
Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.;
"The DNA sequence of human chromosome 7.";
Nature 424:157-164(2003).
[8]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
TISSUE=Colon;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
U95025; AAB72040.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U92459; AAB51764.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AJ236921; CAB36968.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AJ236922; CAB36969.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY608335; AAT37959.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
EU432125; ABY87924.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
EU368948; ABY59657.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
CH236947; EAL24322.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC093725; AAH93725.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC101675; AAI01676.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
IPI IPI00219109; -.
IPI00396012; -.
IPI00413310; -.
RefSeq NP_000836.2; -.
NP_001120795.1; -.
NP_001120798.1; -.
UniGene Hs.449625
3D structure databases
HSSP P23385; 1EWT. [HSSP ENTRY / PDB]
SMR O00222; 38-504.
ModBase O00222.
Protein family/group databases
GPCRDB O00222; GRM8_HUMAN.
PTM databases
PhosphoSite O00222; -.
Organism-specific databases
GeneCards GC07M125865; -.
H-InvDB HIX0025292; -.
HGNC HGNC:4600; GRM8.
GenAtlas GRM8.
MIM 601116; gene. [NCBI / EBI]
PharmGKB PA28997; -.
Gene expression databases
ArrayExpress O00222; -.
Bgee O00222; -.
CleanEx HS_GRM8; -.
GermOnline ENSG00000179603; Homo sapiens.
Ontologies
GO
GO:0005887; Cellular component: integral to plasma membrane (traceable author statement from ProtInc).
GO:0004930; Molecular function: G-protein coupled receptor activity (traceable author statement from UniProtKB).
GO:0008066; Molecular function: glutamate receptor activity (traceable author statement from UniProtKB).
GO:0007186; Biological process: G-protein coupled receptor protein signaling pathway (inferred from electronic annotation from UniProtKB-KW).
GO:0007194; Biological process: negative regulation of adenylate cyclase activity (traceable author statement from ProtInc).
GO:0030818; Biological process: negative regulation of cAMP biosynthetic process (inferred from direct assay from UniProtKB).
GO:0050896; Biological process: response to stimulus (inferred from electronic annotation from UniProtKB-KW).
GO:0007608; Biological process: sensory perception of smell (inferred from electronic annotation from UniProtKB-KW).
GO:0007601; Biological process: visual perception (traceable author statement from ProtInc).
QuickGo view.
Family and domain databases
InterPro IPR001828; ANF_lig_bd_rcpt.
IPR000337; GPCR_3.
IPR017978; GPCR_3_C.
IPR017979; GPCR_3_CS.
IPR000162; GPCR_3_mtglu_rcpt.
IPR000144; GPCR_3_mtglu_rcpt_8.
IPR011500; GPCR_3_NCR.
Graphical view of domain structure.
Pfam PF00003; 7tm_3; 1.
PF01094; ANF_receptor; 1.
PF07562; NCD3G; 1.
Pfam graphical view of domain structure.
PRINTS PR00248; GPCRMGR.
PR01058; MTABOTROPC8R.
PR00593; MTABOTROPICR.
PROSITE PS00979; G_PROTEIN_RECEP_F3_1; 1.
PS00980; G_PROTEIN_RECEP_F3_2; 1.
PS00981; G_PROTEIN_RECEP_F3_3; 1.
PS50259; G_PROTEIN_RECEP_F3_4; 1.
PROSITE graphical view of domain structure (profiles).
Proteomic databases
PRIDE O00222; -.
Genome annotation databases
Ensembl ENSG00000179603; Homo sapiens. [Contig view]
GeneID 2918; -.
KEGG hsa:2918; -.
Phylogenomic databases
HOVERGEN O00222; -.
OMA O00222; NQSGHFL.
Other
DrugBank DB00142; L-Glutamic Acid.
NextBio 11569; -.
SOURCE GRM8; Homo sapiens.
GPCRDB-Snakes O00222.
ProtoNet O00222.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Alternative splicing; Cell membrane; G-protein coupled receptor; Glycoprotein; Isopeptide bond; Membrane; Olfaction; Polymorphism; Receptor; Sensory transduction; Signal; Transducer; Transmembrane; Ubl conjugation.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
SIGNAL   1    33  33     Potential. 
CHAIN   34   908  875     Metabotropic glutamate receptor 8. PRO_0000012941
TOPO_DOM   34   583  550     Extracellular (Potential). 
TRANSMEM   584   608  25     1 (Potential). 
TOPO_DOM   609   620  12     Cytoplasmic (Potential). 
TRANSMEM   621   641  21     2 (Potential). 
TOPO_DOM   642   647  6     Extracellular (Potential). 
TRANSMEM   648   668  21     3 (Potential). 
TOPO_DOM   669   695  27     Cytoplasmic (Potential). 
TRANSMEM   696   716  21     4 (Potential). 
TOPO_DOM   717   746  30     Extracellular (Potential). 
TRANSMEM   747   768  22     5 (Potential). 
TOPO_DOM   769   781  13     Cytoplasmic (Potential). 
TRANSMEM   782   803  22     6 (Potential). 
TOPO_DOM   804   818  15     Extracellular (Potential). 
TRANSMEM   819   843  25     7 (Potential). 
TOPO_DOM   844   908  65     Cytoplasmic (Potential). 
CARBOHYD   95    95        N-linked (GlcNAc...) (Potential). 
CARBOHYD   298   298        N-linked (GlcNAc...) (Potential). 
CARBOHYD   452   452        N-linked (GlcNAc...) (Potential). 
CARBOHYD   480   480        N-linked (GlcNAc...) (Potential). 
CARBOHYD   565   565        N-linked (GlcNAc...) (Potential). 
CROSSLNK   882   882        Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO-1) (By similarity). 
VAR_SEQ   454   501        SAGTPVTFNENGDAPGRYDIFQYQITNKSTEYKVIGHWTN QLHLKVED -> CRRGIQMSLPWPTLFTPSFSSSWAVLALLSLLMKTEMLLD VMISSSIK (in isoform C). VSP_002033
VAR_SEQ   502   908        Missing (in isoform C). VSP_002034
VAR_SEQ   893   908        TSSTKTTYISYSNHSI -> SKSSVEFPMVKSGSTS (in isoform B). VSP_002032
VARIANT   10    10  1     S -> C (in dbSNP:rs769194 [NCBI]). VAR_014446 
VARIANT   21    21  1     F -> C (in dbSNP:rs769202 [NCBI]). VAR_014447 
VARIANT   265   265  1     I -> T (in dbSNP:rs17150343 [NCBI]). VAR_049278 
VARIANT   343   343  1     R -> Q (in dbSNP:rs13309334 [NCBI]). VAR_054752 
VARIANT   362   362  1     F -> Y. VAR_054477 
VARIANT   368   368  1     G -> D. VAR_054478 
VARIANT   392   392  1     R -> Q (in dbSNP:rs2234947 [NCBI]). VAR_014448 
VARIANT   430   430  1     L -> F. VAR_054479 
VARIANT   548   548  1     V -> G (in dbSNP:rs2234948 [NCBI]). VAR_014449 
VARIANT   768   768  1     I -> N (in dbSNP:rs1051433 [NCBI]). VAR_054753 
VARIANT   902   902  1     S -> I (in dbSNP:rs10225567 [NCBI]). VAR_054754 
CONFLICT   194   194        R -> A (in Ref. 1; AAB72040). 
CONFLICT   460   460        T -> I (in Ref. 1; AAB72040). 
CONFLICT   591   591        A -> V (in Ref. 5; ABY87924). 
CONFLICT   642   642        A -> G (in Ref. 1; AAB72040). 
CONFLICT   904   904        S -> T (in Ref. 1; AAB72040). 
Sequence information
Length: 908 AA [This is the length of the unprocessed precursor] Molecular weight: 101741 Da [This is the MW of the unprocessed precursor] CRC64: 95C2D5883DAF6FDE [This is a checksum on the sequence]
        10         20         30         40         50         60 
MVCEGKRSAS CPCFFLLTAK FYWILTMMQR THSQEYAHSI RVDGDIILGG LFPVHAKGER 

        70         80         90        100        110        120 
GVPCGELKKE KGIHRLEAML YAIDQINKDP DLLSNITLGV RILDTCSRDT YALEQSLTFV 

       130        140        150        160        170        180 
QALIEKDASD VKCANGDPPI FTKPDKISGV IGAAASSVSI MVANILRLFK IPQISYASTA 

       190        200        210        220        230        240 
PELSDNTRYD FFSRVVPPDS YQAQAMVDIV TALGWNYVST LASEGNYGES GVEAFTQISR 

       250        260        270        280        290        300 
EIGGVCIAQS QKIPREPRPG EFEKIIKRLL ETPNARAVIM FANEDDIRRI LEAAKKLNQS 

       310        320        330        340        350        360 
GHFLWIGSDS WGSKIAPVYQ QEEIAEGAVT ILPKRASIDG FDRYFRSRTL ANNRRNVWFA 

       370        380        390        400        410        420 
EFWEENFGCK LGSHGKRNSH IKKCTGLERI ARDSSYEQEG KVQFVIDAVY SMAYALHNMH 

       430        440        450        460        470        480 
KDLCPGYIGL CPRMSTIDGK ELLGYIRAVN FNGSAGTPVT FNENGDAPGR YDIFQYQITN 

       490        500        510        520        530        540 
KSTEYKVIGH WTNQLHLKVE DMQWAHREHT HPASVCSLPC KPGERKKTVK GVPCCWHCER 

       550        560        570        580        590        600 
CEGYNYQVDE LSCELCPLDQ RPNMNRTGCQ LIPIIKLEWH SPWAVVPVFV AILGIIATTF 

       610        620        630        640        650        660 
VIVTFVRYND TPIVRASGRE LSYVLLTGIF LCYSITFLMI AAPDTIICSF RRVFLGLGMC 

       670        680        690        700        710        720 
FSYAALLTKT NRIHRIFEQG KKSVTAPKFI SPASQLVITF SLISVQLLGV FVWFVVDPPH 

       730        740        750        760        770        780 
IIIDYGEQRT LDPEKARGVL KCDISDLSLI CSLGYSILLM VTCTVYAIKT RGVPETFNEA 

       790        800        810        820        830        840 
KPIGFTMYTT CIIWLAFIPI FFGTAQSAEK MYIQTTTLTV SMSLSASVSL GMLYMPKVYI 

       850        860        870        880        890        900 
IIFHPEQNVQ KRKRSFKAVV TAATMQSKLI QKGNDRPNGE VKSELCESLE TNTSSTKTTY 


ISYSNHSI 

O00222 in FASTA format

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