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UniProtKB/Swiss-Prot entry O00103


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name UBC11_SCHPO
Primary accession number O00103
Secondary accession numbers None
Integrated into Swiss-Prot on November 1, 1997
Sequence was last modified on July 1, 1997 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 58)
Name and origin of the protein
Protein name Ubiquitin-conjugating enzyme E2-20 kDa
Synonyms EC 6.3.2.19
Ubiquitin-protein ligase
Ubiquitin carrier protein
Gene name
Name: ubc11
Synonyms: ubcp4
ORFNames: SPCC1259.15c
From
Schizosaccharomyces pombe (Fission yeast) [TaxID: 4896] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces.
Protein existence 2: Evidence at transcript level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 38366 / 972;
PubMed=9154838 [NCBI, ExPASy, EBI, Israel, Japan]
Osaka F., Seino H., Seno T., Yamao F.;
"A ubiquitin-conjugating enzyme in fission yeast that is essential for the onset of anaphase in mitosis.";
Mol. Cell. Biol. 17:3388-3397(1997).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 38366 / 972;
DOI=10.1038/nature724; PubMed=11859360 [NCBI, ExPASy, EBI, Israel, Japan]
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M., Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.;
"The genome sequence of Schizosaccharomyces pombe.";
Nature 415:871-880(2002).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
D85545; BAA20375.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
CU329672; CAB38416.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR T40902; T40902.
RefSeq NP_588069.1; -.
3D structure databases
HSSP Q95044; 2E2C. [HSSP ENTRY / PDB]
ModBase O00103.
Enzyme and pathway databases
BioCyc SPOM-XXX-01:SPOM-XXX-01-002391-MON; -.
Organism-specific databases
GeneDB_Spombe SPCC1259.15c; -.
Gene expression databases
ArrayExpress O00103; -.
Ontologies
GO
GO:0005829; Cellular component: cytosol (inferred from direct assay from GeneDB_SPombe).
GO:0005634; Cellular component: nucleus (inferred from direct assay from GeneDB_SPombe).
GO:0004842; Molecular function: ubiquitin-protein ligase activity (traceable author statement from GeneDB_SPombe).
GO:0007059; Biological process: chromosome segregation (inferred from mutant phenotype from GeneDB_SPombe).
GO:0008054; Biological process: cyclin catabolic process (inferred from genetic interaction from GeneDB_SPombe).
GO:0000086; Biological process: G2/M transition of mitotic cell cycle (non-traceable author statement from GeneDB_SPombe).
GO:0007091; Biological process: mitotic metaphase/anaphase transition (inferred from mutant phenotype from GeneDB_SPombe).
GO:0016567; Biological process: protein ubiquitination (traceable author statement from GeneDB_SPombe).
GO:0007346; Biological process: regulation of mitotic cell cycle (inferred from mutant phenotype from GeneDB_SPombe).
QuickGo view.
Family and domain databases
InterPro IPR015582; UbcH10.
IPR016135; UBQ-conjugat/RWD-like.
IPR000608; UBQ-conjugat_E2.
Graphical view of domain structure.
Gene3D G3DSA:3.10.110.10; UBQ-conjugat_E2; 1.
PANTHER PTHR11621:SF26; UbcH10; 1.
PTHR11621; UBQ-conjugat_E2; 1.
Pfam PF00179; UQ_con; 1.
Pfam graphical view of domain structure.
ProDom PD000461; UBQ_conjugat; 1.
[Domain structure / List of seq. sharing at least 1 domain]
SMART SM00212; UBCc; 1.
SMART graphical view of domain structure.
PROSITE PS00183; UBIQUITIN_CONJUGAT_1; 1.
PS50127; UBIQUITIN_CONJUGAT_2; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS O00103.
Genome annotation databases
GeneID 2539190; -.
KEGG spo:SPCC1259.15c; -.
NMPDR fig|4896.1.peg.407; -.
Other
ProtoNet O00103.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Cell cycle; Cell division; Complete proteome; Ligase; Mitosis; Ubl conjugation pathway.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   176  176     Ubiquitin-conjugating enzyme E2-20 kDa. PRO_0000082566
ACT_SITE   113   113        Glycyl thioester intermediate (By similarity). 
Sequence information
Length: 176 AA [This is the length of the unprocessed precursor] Molecular weight: 19664 Da [This is the MW of the unprocessed precursor] CRC64: 6C80769C37AD4950 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MDSDMQNQNP HTNSKNSSSA GMAVDGHSVT KRLRSELMSL MMSNTPGISA FPDSDSNLLH 

        70         80         90        100        110        120 
WAGTITGPSD TYYEGLKFKI SMSFPANYPY SPPTIIFTSP MWHPNVDMSG NICLDILKDK 

       130        140        150        160        170 
WSAVYNVQTI LLSLQSLLGE PNNASPLNAQ AAELWSKDPI EYKRLLMQRY KEIDEI 

O00103 in FASTA format

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View entry in raw text format (no links)
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Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
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