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UniProtKB/Swiss-Prot entry P09229


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name CYT1_ORYSJ
Primary accession number P09229
Secondary accession numbers Q0JIG4 Q547V7 Q7F5W6 Q9SBW0
Integrated into Swiss-Prot on July 1, 1989
Sequence was last modified on February 6, 2007 (Sequence version 2)
Annotations were last modified on    July 22, 2008 (Entry version 73)
Name and origin of the protein
Protein name Cysteine proteinase inhibitor 1 [Precursor]
Synonyms Oryzacystatin-1
Oryzacystatin I
OC-I
SPOC-I
OsCP1
Gene name
OrderedLocusNames: Os01g0803200, LOC_Os01g58890
ORFNames: P0003D09.48, P0034E02.11
From
Oryza sativa subsp. japonica (Rice) [TaxID: 39947] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; Ehrhartoideae; Oryzeae; Oryza.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1016/0378-1119(89)90186-8; PubMed=2806916 [NCBI, ExPASy, EBI, Israel, Japan]
Kondo H., Emori Y., Abe K., Suzuki K., Arai S.;
"Cloning and sequence analysis of the genomic DNA fragment encoding oryzacystatin.";
Gene 81:259-265(1989).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Nipponbare;
DOI=10.1038/nature01184; PubMed=12447438 [NCBI, ExPASy, EBI, Israel, Japan]
Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M., Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
"The genome sequence and structure of rice chromosome 1.";
Nature 420:312-316(2002).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Nipponbare;
DOI=10.1038/nature03895; PubMed=16100779 [NCBI, ExPASy, EBI, Israel, Japan]
International rice genome sequencing project (IRGSP);
"The map-based sequence of the rice genome.";
Nature 436:793-800(2005).
[4]
GENOME REANNOTATION.
STRAIN=cv. Nipponbare;
DOI=10.1101/gr.5509507; PubMed=17210932 [NCBI, ExPASy, EBI, Israel, Japan]
The rice annotation project (RAP);
"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana.";
Genome Res. 17:175-183(2007).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
STRAIN=cv. Nipponbare;
DOI=10.1126/science.1081288; PubMed=12869764 [NCBI, ExPASy, EBI, Israel, Japan]
The rice full-length cDNA consortium;
"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice.";
Science 301:376-379(2003).
[6]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-63.
PubMed=10594126 [NCBI, ExPASy, EBI, Israel, Japan]
Womack J.S., Randall J., Kemp J.D.;
"Oryzacystatin-I has a signal peptide.";
(er) Plant Gene Register PGR99-175.
[7]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-63.
DOI=10.1007/s004250050688; PubMed=10805458 [NCBI, ExPASy, EBI, Israel, Japan]
Womack J.S., Randall J., Kemp J.D.;
"Identification of a signal peptide for oryzacystatin-I.";
Planta 210:844-847(2000).
[8]
NUCLEOTIDE SEQUENCE [MRNA] OF 11-140 (ISOFORM 1).
TISSUE=Seed;
PubMed=8877110 [NCBI, ExPASy, EBI, Israel, Japan]
Zhou Z., Zhu Z., Chen R., Liu C., Li X.;
"High level expression of oryzacystatin in Escherichia coli.";
Chin. J. Biotechnol. 12:17-24(1996).
[9]
NUCLEOTIDE SEQUENCE [MRNA] OF 12-140 (ISOFORM 1).
STRAIN=cv. Aichiasahi;
Peng Y.L., Qin Q.M., Su S.C., Li H.;
"OsCPI, a rice blast inducible cysteine proteinase inhibitor.";
Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases.
[10]
NUCLEOTIDE SEQUENCE [MRNA] OF 17-140 (ISOFORM 1).
DOI=10.1016/1046-5928(92)90054-Z; PubMed=1422207 [NCBI, ExPASy, EBI, Israel, Japan]
Chen M.S., Johnson B., Wen L., Muthukrishnan S., Kramer K.J., Morgan T.D., Reeck G.R.;
"Rice cystatin: bacterial expression, purification, cysteine proteinase inhibitory activity, and insect growth suppressing activity of a truncated form of the protein.";
Protein Expr. Purif. 3:41-49(1992).
[11]
NUCLEOTIDE SEQUENCE [MRNA] OF 26-140 (ISOFORM 1), AND PARTIAL PROTEIN SEQUENCE.
STRAIN=cv. Nipponbare;
TISSUE=Seed;
PubMed=3500172 [NCBI, ExPASy, EBI, Israel, Japan]
Abe K., Emori Y., Kondo H., Suzuki K., Arai S.;
"Molecular cloning of a cysteine proteinase inhibitor of rice (oryzacystatin). Homology with animal cystatins and transient expression in the ripening process of rice seeds.";
J. Biol. Chem. 262:16793-16797(1987).
[12]
FUNCTION, AND DEVELOPMENTAL STAGE.
PubMed=1697595 [NCBI, ExPASy, EBI, Israel, Japan]
Kondo H., Abe K., Nishimura I., Watanabe H., Emori Y., Arai S.;
"Two distinct cystatin species in rice seeds with different specificities against cysteine proteinases. Molecular cloning, expression, and biochemical studies on oryzacystatin-II.";
J. Biol. Chem. 265:15832-15837(1990).
[13]
MUTAGENESIS OF ASP-124.
PubMed=7655503 [NCBI, ExPASy, EBI, Israel, Japan]
Urwin P.E., Atkinson H.J., Waller D.A., McPherson M.J.;
"Engineered oryzacystatin-I expressed in transgenic hairy roots confers resistance to Globodera pallida.";
Plant J. 8:121-131(1995).
[14]
GENE FAMILY.
DOI=10.1007/s00438-005-1147-4; PubMed=15887031 [NCBI, ExPASy, EBI, Israel, Japan]
Martinez M., Abraham Z., Carbonero P., Diaz I.;
"Comparative phylogenetic analysis of cystatin gene families from arabidopsis, rice and barley.";
Mol. Genet. Genomics 273:423-432(2005).
[15]
STRUCTURE BY NMR OF 39-140.
DOI=10.1021/bi0006971; PubMed=11101290 [NCBI, ExPASy, EBI, Israel, Japan]
Nagata K., Kudo N., Abe K., Arai S., Tanokura M.;
"Three-dimensional solution structure of oryzacystatin-I, a cysteine proteinase inhibitor of the rice, Oryza sativa L. japonica.";
Biochemistry 39:14753-14760(2000).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M29259; AAA33912.1; ALT_INIT; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AP003221; BAB86438.1; ALT_INIT; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AP003232; BAB92242.1; ALT_INIT; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AP008207; BAF06464.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK061770; -; NOT_ANNOTATED_CDS; mRNA.[EMBL / GenBank / DDBJ]
AF164378; AAF18388.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U54702; AAB66355.1; ALT_FRAME; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF435976; AAL30830.1; ALT_INIT; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
S49967; AAB24010.1; ALT_INIT; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
J03469; AAA33903.1; ALT_INIT; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR B28464; A28464.
RefSeq NP_001044550.1; -.
UniGene Os.1316
3D structure databases
PDB
1EQK; NMR; -; A=39-140.[ExPASy / RCSB / EBI]
PDBsum 1EQK; -.
ModBase P09229.
Protein family/group databases
MEROPS I25.014; -.
Organism-specific databases
Gramene P09229; -.
Family and domain databases
InterPro IPR000010; Prot_inh_cystat.
Graphical view of domain structure.
Pfam PF00031; Cystatin; 1.
Pfam graphical view of domain structure.
ProDom PD001231; Prot_inh_I25A_B; 1.
[Domain structure / List of seq. sharing at least 1 domain]
SMART SM00043; CY; 1.
SMART graphical view of domain structure.
PROSITE PS00287; CYSTATIN; 1.
BLOCKS P09229.
Genome annotation databases
GeneID 4327535; -.
KEGG osa:4327535; -.
Other
ProtoNet P09229.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Alternative splicing; Direct protein sequencing; Plant defense; Protease inhibitor; Secreted; Signal; Thiol protease inhibitor.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
SIGNAL   1    26  26     Potential. 
CHAIN   27   140  114     Cysteine proteinase inhibitor 1. PRO_0000207156
DOMAIN   48   135  88     Cystatin. 
MOTIF   91    95  5     Secondary area of contact. 
SITE   48    48  1     Reactive site. 
VAR_SEQ   77    96        NSLLEFEKLVSVKQQVVAGT -> VSAPLYPPPYFSSSQGRSSN (in isoform 2). VSP_023023
VAR_SEQ   97   140        Missing (in isoform 2). VSP_023024
MUTAGEN   124   124        Missing: Increase in inhibition activity by 13-fold. 
STRAND   47    53  7      
HELIX   59    75  17      
STRAND   80   106  27      
STRAND   111   120  10      
TURN   121   124  4      
STRAND   128   132  5      
Sequence information
Length: 140 AA [This is the length of the unprocessed precursor] Molecular weight: 15355 Da [This is the MW of the unprocessed precursor] CRC64: 541263986E70C8FB [This is a checksum on the sequence]
        10         20         30         40         50         60 
MRKYRVAGLV AALLVLHSLA TPSAQAEAHR AGGEGEEKMS SDGGPVLGGV EPVGNENDLH 

        70         80         90        100        110        120 
LVDLARFAVT EHNKKANSLL EFEKLVSVKQ QVVAGTLYYF TIEVKEGDAK KLYEAKVWEK 

       130        140 
PWMDFKELQE FKPVDASANA 

P09229 in FASTA format

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