FUNCTION: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, leading to derepression of the SOS regulon and eventually DNA repair (By similarity).
CATALYTIC ACTIVITY: Hydrolysis of Ala-|-Gly bond in repressor lexA.
SUBUNIT: Homodimer (By similarity).
SIMILARITY: Belongs to the peptidase S24 family [view classification].

Copyright

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Cross-references

Sequence databases

EMBL

CP000927; ABZ71909.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_001684407.1; -.

3D structure databases

ModBase

B0SYZ8.

Protein family/group databases

MEROPS

S24.001; -.

Ontologies

GO:0003677;	Molecular function: DNA binding (inferred from electronic annotation from HAMAP).
GO:0004252;	Molecular function: serine-type endopeptidase activity (inferred from electronic annotation from HAMAP).
GO:0006281;	Biological process: DNA repair (inferred from electronic annotation from HAMAP).
GO:0006260;	Biological process: DNA replication (inferred from electronic annotation from HAMAP).
GO:0045892;	Biological process: negative regulation of transcription, DNA-dependent (inferred from electronic annotation from HAMAP).
GO:0009432;	Biological process: SOS response (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00015; -; 1.
PBIL [Tree]

InterPro

IPR006199; LexA_DNA_bd.
IPR006200; Pept_S24_LexA.
IPR006197; Pept_S24_SOS.
IPR011056; Peptidase_S24_S26_C.
IPR011991; Wing_hlx_DNA_bd.
Graphical view of domain structure.

Gene3D

G3DSA:2.10.109.10; Pept_S24_S26_C; 1.
G3DSA:1.10.10.10; Wing_hlx_DNA_bd; 1.

Pfam

PF01726; LexA_DNA_bind; 1.
PF00717; Peptidase_S24; 1.
Pfam graphical view of domain structure.

Genome annotation databases

GeneID

5900237; -.

GenomeReviews

CP000927_GR; Caul_2782.

KEGG

cak:Caul_2782; -.

CMR

B0SYZ8; Caul_2782.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Autocatalytic cleavage; Complete proteome; DNA damage; DNA repair; DNA replication; DNA-binding; Hydrolase; Repressor; SOS response; Transcription; Transcription regulation.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 235`	`235`	`LexA repressor.`	`PRO_1000074050`
`DNA_BIND`	`26 46`	`21`	`H-T-H motif (By similarity).`
`ACT_SITE`	`156 156`		`For autocatalytic cleavage activity (By similarity).`
`ACT_SITE`	`193 193`		`For autocatalytic cleavage activity (By similarity).`
`SITE`	`121 122`	`2`	`Cleavage; by autolysis (By similarity).`

Sequence information

Length: 235 AA [This is the length of the unprocessed precursor]

Molecular weight: 25650 Da [This is the MW of the unprocessed precursor]

CRC64: 5686CC84E3A4BF22 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MLTRKQHELL MFIHERIKES GVSPSFDEMK EALDLASKSG IHRLITALEE RGFIRRLAHR 

        70         80         90        100        110        120 
ARALEVVKLP QQATTAAPPK GRGAFRPQVL EGGGQAPTTS AQPQMAADNS RELPILGRIA 

       130        140        150        160        170        180 
AGTPIDAIQH ERERLPVPES MLGAGEHYVL EVQGDSMIEA GILDGDYVII KKGDTANSGE 

       190        200        210        220        230 
IVVALVGEEA TLKRLRKKGG SIALEAANPK YETRIFGPDQ VEVQGKLVGL IRRYH

B0SYZ8 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools