NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1371/journal.pone.0001450; PubMed=18197260 [NCBI, ExPASy, EBI, Israel, Japan]
Xu Z., Zhou Y., Li L., Zhou R., Xiao S., Wan Y., Zhang S., Wang K., Li W., Li L., Jin H., Kang M., Dalai B., Li T., Liu L., Cheng Y., Zhang L., Xu T., Zheng H., Pu S., Wang B., Gu W., Zhang X.L., Zhu G.-F., Wang S., Zhao G.-P., Chen H.;
"Genome biology of Actinobacillus pleuropneumoniae JL03, an isolate of serotype 3 prevalent in China.";
PLoS ONE 3:E1450-E1450(2008).

Comments

FUNCTION: Cell wall formation (By similarity).
CATALYTIC ACTIVITY: ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.
COFACTOR: Binds 2 magnesium or manganese ions per subunit (By similarity).
PATHWAY: Cell wall biosynthesis; peptidoglycan biosynthesis .
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
SIMILARITY: Belongs to the D-alanine--D-alanine ligase family.
SIMILARITY: Contains 1 ATP-grasp domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CP000687; ABY68627.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_001651071.1; -.

3D structure databases

ModBase

B0BRH9.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0008716;	Molecular function: D-alanine-D-alanine ligase activity (inferred from electronic annotation from HAMAP).
GO:0009252;	Biological process: peptidoglycan biosynthetic process (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00047; -; 1.
PBIL [Tree]

InterPro

IPR011761; ATP-grasp.
IPR013816; ATP_grasp_subdomain_2.
IPR005905; D_ala_D_ala.
IPR011095; Dala_Dala_lig_C.
IPR011127; Dala_Dala_lig_N.
IPR013817; Pre-ATP_grasp.
Graphical view of domain structure.

Gene3D

G3DSA:3.30.470.20; ATP_grasp_subdomain_2; 1.
G3DSA:3.40.50.20; Pre-ATP_grasp; 1.

Pfam

PF07478; Dala_Dala_lig_C; 1.
PF01820; Dala_Dala_lig_N; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR01205; D_ala_D_alaTIGR; 1.

PROSITE

PS50975; ATP_GRASP; 1.
PS00843; DALA_DALA_LIGASE_1; 1.
PS00844; DALA_DALA_LIGASE_2; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

B0BRH9.

Genome annotation databases

GeneID

5851576; -.

GenomeReviews

CP000687_GR; APJL_0021.

KEGG

apj:APJL_0021; -.

CMR

B0BRH9; APJL_0021.

Other

ProtoNet

B0BRH9.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; Cell shape; Cell wall biogenesis/degradation; Complete proteome; Cytoplasm; Ligase; Magnesium; Manganese; Metal-binding; Nucleotide-binding; Peptidoglycan synthesis.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 303`	`303`	`D-alanine--D-alanine ligase.`	`PRO_0000341047`
`DOMAIN`	`104 300`	`197`	`ATP-grasp.`
`NP_BIND`	`132 187`	`56`	`ATP (By similarity).`
`METAL`	`254 254`		`Magnesium or manganese 1 (By similarity).`
`METAL`	`267 267`		`Magnesium or manganese 1 (By similarity).`
`METAL`	`267 267`		`Magnesium or manganese 2 (By similarity).`
`METAL`	`269 269`		`Magnesium or manganese 2 (By similarity).`

Sequence information

Length: 303 AA [This is the length of the unprocessed precursor]

Molecular weight: 32704 Da [This is the MW of the unprocessed precursor]

CRC64: 620151A5C69B3D2E [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSIKDEKIAV LFGGVSQERE VSLNSGAAVT EALKSLGYNV EGIDTKDFPI EKLKEKGIQR 

        70         80         90        100        110        120 
VFNILHGGIG ENGVLQGALE QMGIPYTGCG VMASAVTLDK FRTKLMWQAV GLPTADMVVV 

       130        140        150        160        170        180 
RRGEAVDSEQ IIAKLGLPVF VKPSSEGSSV GVTKVKTVEQ LLPAVEEALK FDSIVLVEAF 

       190        200        210        220        230        240 
LAGKEYSVPV LDGQVLPAVQ IIPEGEFYDY HAKYISDNTQ YLVPALSDDR QAEVAELVKA 

       250        260        270        280        290        300 
AYEVVGCRGW SRIDVMEDAN GHFNLVEVNT CPGMTSHSIF PKSAATVGIP FEKLVERVLE 


LSA

B0BRH9 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools