Peptidylglycine alpha-hydroxylating monooxygenase
     (EC 1.14.17.3)
     (PHM)
Peptidyl-alpha-hydroxyglycine alpha-amidating lyase
     (EC 4.3.2.5)
     (Peptidylamidoglycolate lyase)
     (PAL)

Gene name

Name:

Pam

From

Rattus norvegicus (Rat)

[TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

Protein existence

1: Evidence at protein level;

References

[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (PAM-1/2). STRAIN=Sprague-Dawley; TISSUE=Heart atrium; PubMed=2911604 [NCBI, ExPASy, EBI, Israel, Japan] Stoffers D.A., Green C.B.R., Eipper B.A.; "Alternative mRNA splicing generates multiple forms of peptidyl-glycine alpha-amidating monooxygenase in rat atrium."; Proc. Natl. Acad. Sci. U.S.A. 86:735-739(1989).
[2]	NUCLEOTIDE SEQUENCE [MRNA] (PAM-3/4). STRAIN=Sprague-Dawley; TISSUE=Heart atrium; PubMed=1988445 [NCBI, ExPASy, EBI, Israel, Japan] Stoffers D.A., Ouafik L., Eipper B.A.; "Characterization of novel mRNAs encoding enzymes involved in peptide alpha-amidation."; J. Biol. Chem. 266:1701-1707(1991).
[3]	NUCLEOTIDE SEQUENCE [MRNA] (PAM-1 TO 5). STRAIN=Wistar; TISSUE=Pituitary; DOI=10.1016/0014-5793(90)81184-P; PubMed=2401356 [NCBI, ExPASy, EBI, Israel, Japan] Kato I., Yonekura H., Yamamoto H., Okamoto H.; "Isolation and functional expression of pituitary peptidylglycine alpha-amidating enzyme mRNA. A variant lacking the transmembrane domain."; FEBS Lett. 269:319-323(1990).
[4]	NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (PAM-4). STRAIN=Sprague-Dawley; TISSUE=Liver; DOI=10.1016/0003-9861(90)90466-C; PubMed=2337358 [NCBI, ExPASy, EBI, Israel, Japan] Bertelsen A.H., Beaudry G.A., Galella E.A., Jones B.N., Ray M.L., Mehta N.M.; "Cloning and characterization of two alternatively spliced rat alpha-amidating enzyme cDNAs from rat medullary thyroid carcinoma."; Arch. Biochem. Biophys. 279:87-96(1990).
[5]	NUCLEOTIDE SEQUENCE [MRNA], AND ALTERNATIVE SPLICING. PubMed=1740449 [NCBI, ExPASy, EBI, Israel, Japan] Eipper B.A., Green C.B., Campbell T.A., Stoffers D.A., Keutmann H.T., Mains R.E., Ouafik L.; "Alternative splicing and endoproteolytic processing generate tissue-specific forms of pituitary peptidylglycine alpha-amidating monooxygenase (PAM)."; J. Biol. Chem. 267:4008-4015(1992).
[6]	PROTEIN SEQUENCE OF 26-42, AND GLYCOSYLATION AT ASN-765. PubMed=2211657 [NCBI, ExPASy, EBI, Israel, Japan] Beaudry G.A., Mehta N.M., Ray M.L., Bertelsen A.H.; "Purification and characterization of functional recombinant alpha-amidating enzyme secreted from mammalian cells."; J. Biol. Chem. 265:17694-17699(1990).
[7]	ALTERNATIVE SPLICING. DOI=10.1210/me.6.10.1571; PubMed=1448112 [NCBI, ExPASy, EBI, Israel, Japan] Ouafik L.H., Stoffers D.A., Campbell T.A., Johnson R.C., Bloomquist B.T., Mains R.E., Eipper B.A.; "The multifunctional peptidylglycine alpha-amidating monooxygenase gene: exon/intron organization of catalytic, processing, and routing domains."; Mol. Endocrinol. 6:1571-1584(1992).
[8]	INTERACTION WITH PAMCI. DOI=10.1074/jbc.273.50.33524; PubMed=9837933 [NCBI, ExPASy, EBI, Israel, Japan] Chen L., Johnson R.C., Milgram S.L.; "P-CIP1, a novel protein that interacts with the cytosolic domain of peptidylglycine alpha-amidating monooxygenase, is associated with endosomes."; J. Biol. Chem. 273:33524-33532(1998).
[9]	DISULFIDE BONDS IN CATALYTIC DOMAIN. DOI=10.1021/bi0260280; PubMed=12369828 [NCBI, ExPASy, EBI, Israel, Japan] Kolhekar A.S., Bell J., Shiozaki E.N., Jin L., Keutmann H.T., Hand T.A., Mains R.E., Eipper B.A.; "Essential features of the catalytic core of peptidyl-alpha-hydroxyglycine alpha-amidating lyase."; Biochemistry 41:12384-12394(2002).
[10]	X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 45-354. DOI=10.1126/science.278.5341.1300; PubMed=9360928 [NCBI, ExPASy, EBI, Israel, Japan] Prigge S.T., Kolhekar A.S., Eipper B.A., Mains R.E., Amzel L.M.; "Amidation of bioactive peptides: the structure of peptidylglycine alpha-hydroxylating monooxygenase."; Science 278:1300-1305(1997).

Comments

FUNCTION: Bifunctional enzyme that catalyzes 2 sequencial steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity.
CATALYTIC ACTIVITY: Peptidylglycine + ascorbate + O₂ = peptidyl(2-hydroxyglycine) + dehydroascorbate + H₂O.
CATALYTIC ACTIVITY: Peptidylamidoglycolate = peptidyl amide + glyoxylate.
COFACTOR: Zinc; for the lyase reaction.
COFACTOR: Binds 2 copper ions per subunit; For the monoxygenase reaction.
SUBUNIT: Monomer. Interacts with PAMCI.
INTERACTION:
O88869:Pamci; NbExp=1; IntAct=EBI-1395008, EBI-1395057;
SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle membrane; Single-pass membrane protein. Note=Secretory granules.

ALTERNATIVE PRODUCTS: 7 named isoforms [FASTA] produced by alternative splicing.

Name	PAM-1
Isoform ID	P14925-1
Note: Membrane-bound.
This is the isoform sequence displayed in this entry.

Name	PAM-2
Isoform ID	P14925-2
Note: Membrane-bound.
Features which should be applied to build the isoform sequence: VSP_001232.

Name	PAM-3
Isoform ID	P14925-3
Note: Soluble.
Features which should be applied to build the isoform sequence: VSP_001232, VSP_001234.

Name	PAM-3A
Isoform ID	P14925-4
Note: Soluble.
Features which should be applied to build the isoform sequence: VSP_001232, VSP_001233.

Name	PAM-3B
Isoform ID	P14925-5
Note: Membrane-bound.
Features which should be applied to build the isoform sequence: VSP_001232, VSP_001235.

Name	PAM-4
Isoform ID	P14925-6
Note: Soluble.
Features which should be applied to build the isoform sequence: VSP_001236, VSP_001237.

Name	PAM-5
Isoform ID	P14925-7
Note: Soluble.
Features which should be applied to build the isoform sequence: VSP_001230, VSP_001231.

SIMILARITY: In the C-terminal section; belongs to the peptidyl-alpha-hydroxyglycine alpha-amidating lyase family.
SIMILARITY: In the N-terminal section; belongs to the copper type II ascorbate-dependent monooxygenase family.
SIMILARITY: Contains 5 NHL repeats.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

U52650; AAC05602.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52639; AAC05602.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52640; AAC05602.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52641; AAC05602.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52642; AAC05602.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52643; AAC05602.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52644; AAC05602.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52645; AAC05602.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52646; AAC05602.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52647; AAC05602.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52648; AAC05602.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52649; AAC05602.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52653; AAC05603.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52639; AAC05603.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52640; AAC05603.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52641; AAC05603.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52642; AAC05603.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52643; AAC05603.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52644; AAC05603.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52645; AAC05603.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52646; AAC05603.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52647; AAC05603.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52648; AAC05603.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52649; AAC05603.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52651; AAC05603.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52652; AAC05603.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52664; AAC05607.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52639; AAC05607.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52640; AAC05607.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52641; AAC05607.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52642; AAC05607.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52643; AAC05607.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52644; AAC05607.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52645; AAC05607.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52646; AAC05607.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52647; AAC05607.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52648; AAC05607.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52649; AAC05607.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52651; AAC05607.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52652; AAC05607.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52653; AAC05607.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52654; AAC05607.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52655; AAC05607.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52656; AAC05607.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52657; AAC05607.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52658; AAC05607.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52659; AAC05607.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52660; AAC05607.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52661; AAC05607.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52662; AAC05607.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52663; AAC05607.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52664; AAC05605.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52639; AAC05605.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52640; AAC05605.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52641; AAC05605.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52642; AAC05605.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52643; AAC05605.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52644; AAC05605.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52645; AAC05605.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52646; AAC05605.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52647; AAC05605.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52648; AAC05605.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52649; AAC05605.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52651; AAC05605.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52652; AAC05605.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52654; AAC05605.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52655; AAC05605.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52656; AAC05605.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52657; AAC05605.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52658; AAC05605.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52659; AAC05605.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52660; AAC05605.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52661; AAC05605.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52662; AAC05605.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52663; AAC05605.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52664; AAC05604.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52639; AAC05604.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52640; AAC05604.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52641; AAC05604.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52642; AAC05604.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52643; AAC05604.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52644; AAC05604.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52645; AAC05604.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52646; AAC05604.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52647; AAC05604.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52648; AAC05604.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52649; AAC05604.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52651; AAC05604.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52652; AAC05604.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52654; AAC05604.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52655; AAC05604.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52656; AAC05604.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52657; AAC05604.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52658; AAC05604.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52659; AAC05604.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52660; AAC05604.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52661; AAC05604.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52662; AAC05604.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52664; AAC05608.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52639; AAC05608.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52640; AAC05608.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52641; AAC05608.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52642; AAC05608.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52643; AAC05608.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52644; AAC05608.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52645; AAC05608.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52646; AAC05608.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52647; AAC05608.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52648; AAC05608.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52649; AAC05608.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52651; AAC05608.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52652; AAC05608.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52654; AAC05608.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52655; AAC05608.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52656; AAC05608.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52657; AAC05608.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52658; AAC05608.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52659; AAC05608.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52660; AAC05608.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52661; AAC05608.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52664; AAC05606.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52639; AAC05606.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52640; AAC05606.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52641; AAC05606.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52642; AAC05606.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52643; AAC05606.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52644; AAC05606.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52645; AAC05606.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52646; AAC05606.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52647; AAC05606.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52648; AAC05606.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52649; AAC05606.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52651; AAC05606.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52652; AAC05606.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52654; AAC05606.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52655; AAC05606.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52656; AAC05606.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52657; AAC05606.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52658; AAC05606.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52659; AAC05606.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52660; AAC05606.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52661; AAC05606.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U52663; AAC05606.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
M25732; AAA41803.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
M25719; AAA41804.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
M63662; AAA42068.1; ALT_SEQ; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
X59685; CAA42206.1; ALT_SEQ; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
X59686; CAA42207.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
X59687; CAA42208.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
X59688; CAA42209.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
X59689; CAA42210.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
M82845; AAB00162.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

A32193; URRTAP.

NP_037132.2; -.

3D structure databases

PDB

1OPM; X-ray; 2.10 A; A=45-354.	[ExPASy / RCSB / EBI]
1PHM; X-ray; 1.90 A; A=45-354.	[ExPASy / RCSB / EBI]
1SDW; X-ray; 1.85 A; A=43-356.	[ExPASy / RCSB / EBI]
1YI9; X-ray; 1.70 A; A=47-355.	[ExPASy / RCSB / EBI]
1YIP; X-ray; 2.20 A; A=45-355.	[ExPASy / RCSB / EBI]
1YJK; X-ray; 2.00 A; A=50-355.	[ExPASy / RCSB / EBI]
1YJL; X-ray; 2.40 A; A=50-355.	[ExPASy / RCSB / EBI]
3PHM; X-ray; 2.10 A; A=45-354.	[ExPASy / RCSB / EBI]

Detailed list of linked structures.

PDBsum

1OPM; -.
1PHM; -.
1SDW; -.
1YI9; -.
1YIP; -.
1YJK; -.
1YJL; -.
3PHM; -.

ModBase

P14925.

Protein-protein interaction databases

IntAct

P14925; -.

PTM databases

PhosphoSite

P14925; -.

Organism-specific databases

RGD

3252; Pam.

GeneLynx

Pam; Rattus norvegicus.

Gene expression databases

ArrayExpress

P14925; -.

GermOnline

ENSRNOG00000033280; Rattus norvegicus.

Ontologies

GO:0005515;	Molecular function: protein binding (inferred from physical interaction from UniProtKB).
QuickGo view.

Family and domain databases

InterPro

IPR011042; 6-blade_b-propeller_TolB-like.
IPR014783; Cu2_ascorb_mOase_C.
IPR014784; Cu2_ascorb_mOase_like_C.
IPR000323; Cu2_ascorb_mOase_N.
IPR001258; NHL_repeat.
IPR013017; NHL_repeat_subgr.
IPR000720; Pep_amidat_mOase.
Graphical view of domain structure.

Gene3D

G3DSA:2.120.10.30; 6-blade_b-propeller_TolB-like; 1.
G3DSA:2.60.120.230; Cu2_ascorb_mOase_core; 1.
G3DSA:2.60.120.310; Cu2_ascorb_mOase_core; 1.

Pfam

PF03712; Cu2_monoox_C; 1.
PF01082; Cu2_monooxygen; 1.
PF01436; NHL; 4.
Pfam graphical view of domain structure.

PRINTS

PR00790; PAMONOXGNASE.

PROSITE

PS00084; CU2_MONOOXYGENASE_1; 1.
PS00085; CU2_MONOOXYGENASE_2; 1.
PS51125; NHL; 5.
PROSITE graphical view of domain structure (profiles).

BLOCKS

P14925.

Genome annotation databases

Ensembl

ENSRNOG00000033280; Rattus norvegicus. [Contig view]

GeneID

25508; -.

KEGG

rno:25508; -.

Other

P14925; -.

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

3D-structure; Alternative splicing; Cleavage on pair of basic residues; Copper; Cytoplasmic vesicle; Direct protein sequencing; Glycoprotein; Lyase; Membrane; Metal-binding; Monooxygenase; Multifunctional enzyme; Oxidoreductase; Phosphoprotein; Repeat; Signal; Sulfation; Transmembrane; Vitamin C; Zinc.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 25`	`25`
`PROPEP`	`26 35`	`10`		`PRO_0000006365`
`CHAIN`	`36 976`	`941`	`Peptidyl-glycine alpha-amidating monooxygenase.`	`PRO_0000006366`
`TOPO_DOM`	`36 866`	`831`	`Intragranular (Potential).`
`TRANSMEM`	`867 890`	`24`	`Potential.`
`TOPO_DOM`	`891 976`	`86`	`Cytoplasmic (Potential).`
`REPEAT`	`501 544`	`44`	`NHL 1.`
`REPEAT`