[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. McClelland M., Sanderson E.K., Porwollik S., Spieth J., Clifton W.S., Fulton R., Cordes M., Wollam A., Shah N., Pepin K., Bhonagiri V., Nash W., Johnson M., Thiruvilangam P., Wilson R.; Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Catalyzes the decarboxylation of 3-keto-L-gulonate-6-P into L-xylulose-5-P. Is involved in the anaerobic L-ascorbate utilization (By similarity).
CATALYTIC ACTIVITY: 3-dehydro-L-gulonate 6-phosphate = L-xylulose 5-phosphate + CO₂.
COFACTOR: Binds 1 magnesium ion per subunit (By similarity).
PATHWAY: Cofactor degradation; L-ascorbate degradation; D-xylulose 5-phosphate from L-ascorbate: step 2/4 .
SUBUNIT: Homodimer (By similarity).
INDUCTION: Induced by L-ascorbate. Repressed by ulaR (By similarity).
SIMILARITY: Belongs to the HPS/KGPDC family. KGPDC subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CP000886; ABX70789.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_001591622.1; -.

3D structure databases

ModBase

A9N512.

Ontologies

GO:0033982;	Molecular function: 3-dehydro-L-gulonate-6-phosphate decarboxylase activity (inferred from electronic annotation from EC).
GO:0000287;	Molecular function: magnesium ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0005975;	Biological process: carbohydrate metabolic process (inferred from electronic annotation from UniProtKB-KW).
GO:0019854;	Biological process: L-ascorbic acid catabolic process (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_01267; -; 1.
PBIL [Tree]

InterPro

IPR013785; Aldolase_TIM.
Graphical view of domain structure.

Gene3D

G3DSA:3.20.20.70; Aldolase_TIM; 1.

Genome annotation databases

GeneID

5779128; -.

GenomeReviews

CP000886_GR; SPAB_05520.

KEGG

spq:SPAB_05520; -.

CMR

A9N512; SPAB_05520.

Other

ProtoNet

A9N512.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Carbohydrate metabolism; Complete proteome; Decarboxylase; Lyase; Magnesium; Metal-binding.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 216`	`216`	`3-keto-L-gulonate-6-phosphate decarboxylase ulaD.`	`PRO_1000085827`
`METAL`	`33 33`		`Magnesium (By similarity).`
`METAL`	`62 62`		`Magnesium (By similarity).`
`BINDING`	`11 11`		`Substrate (By similarity).`
`BINDING`	`192 192`		`Substrate (By similarity).`
`SITE`	`64 64`	`1`	`Transition state stabilizer (By similarity).`
`SITE`	`67 67`	`1`	`Transition state stabilizer (By similarity).`

Sequence information

Length: 216 AA [This is the length of the unprocessed precursor]

Molecular weight: 23706 Da [This is the MW of the unprocessed precursor]

CRC64: CDF280DC1D1BAD68 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSLPMLQVAL DNQTMDSAYE TTRLIAEEVD IIEVGTILCV GEGVRAVRDL KALYPHKIVL 

        70         80         90        100        110        120 
ADAKIADAGK ILSRMCFEAN ADWVTVICCA DINTAKGALD VAKEFNGDVQ IELTGYWTWE 

       130        140        150        160        170        180 
QAQQWRDAGI QQVVYHRSRD AQAAGVAWGE ADITAIKRLS DMGFKVTVTG GLALEDLPLF 

       190        200        210 
KGIPIHVFIA GRSIRDAESP VEAARQFKRS IAQLWG

A9N512 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools