FUNCTION: Cleaves proteins, imported into the mitochondrion, to their mature size. While most mitochondrial precursor proteins are processed to the mature form in one step by mitochondrial processing peptidase (MPP), the sequential cleavage by MIP of an octapeptide after initial processing by MPP is a required step for a subgroup of nuclear-encoded precursor proteins destined for the matrix or the inner membrane (By similarity).
CATALYTIC ACTIVITY: Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.
COFACTOR: Binds 1 zinc ion (By similarity).
SUBCELLULAR LOCATION: Mitochondrion matrix (By similarity).
SIMILARITY: Belongs to the peptidase M3 family [view classification].

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AACS01000026; EAU92790.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

XP_001829155.1; -.

3D structure databases

ModBase

A8N2T3.

Protein family/group databases

MEROPS

M03.006; -.

Ontologies

GO:0005759;	Cellular component: mitochondrial matrix (inferred from electronic annotation from UniProtKB-SubCell).
GO:0004222;	Molecular function: metalloendopeptidase activity (inferred from electronic annotation from InterPro).
GO:0008270;	Molecular function: zinc ion binding (inferred from electronic annotation from InterPro).
GO:0006508;	Biological process: proteolysis (inferred from electronic annotation from InterPro).
QuickGo view.

Family and domain databases

InterPro

IPR001567; Pept_M3A_M3B.
IPR006025; Pept_M_Zn_BS.
Graphical view of domain structure.

Pfam

PF01432; Peptidase_M3; 1.
Pfam graphical view of domain structure.

PROSITE

PS00142; ZINC_PROTEASE; 1.

Genome annotation databases

GeneID

6005581; -.

Other

ProtoNet

A8N2T3.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Hydrolase; Metal-binding; Metalloprotease; Mitochondrion; Protease; Transit peptide; Zinc.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 39`	`39`	`Mitochondrion (Potential).`
`CHAIN`	`40 777`	`738`	`Mitochondrial intermediate peptidase.`	`PRO_0000338580`
`COMPBIAS`	`490 493`	`4`	`Poly-Asp.`
`ACT_SITE`	`562 562`		`By similarity.`
`METAL`	`561 561`		`Zinc; catalytic (By similarity).`
`METAL`	`565 565`		`Zinc; catalytic (By similarity).`
`METAL`	`568 568`		`Zinc; catalytic (By similarity).`

Sequence information

Length: 777 AA [This is the length of the unprocessed precursor]

Molecular weight: 87437 Da [This is the MW of the unprocessed precursor]

CRC64: 1BE83120B6ECF1B2 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MLSSSARTVL ARHSARQLYR FRGCLVHQQR HRHQVQRTLA THVQRHLPAS LDDKALVALF 

        70         80         90        100        110        120 
DQPNGSKLRS PFNTTGLFGH PNLTHPRALV SLAESTLVRA QLLTQRILEA GKSEHELAKV 

       130        140        150        160        170        180 
VKNLDRLSDM LCGVIDLAEL VRNAHPDRLW VEAANHAYET LCEFMNVLNT HTGLYEVLKQ 

       190        200        210        220        230        240 
VLSNPTLVNS LSPEAYQTAL IFWRDFEKSA IDLPPAQRNK FVSLSSDILV LGRQFLENAS 

       250        260        270        280        290        300 
TPRPPTSIKA SDLAGLKDKG MGVRLQLQAQ FTNRDLQIYP GSLQAHMIMR SAPNEEPRRR 

       310        320        330        340        350        360 
LYLAANSSTQ EQIEVLEALL KKRAELAQLV GRESFAHMTL DDKMAKTPDN VTNFLDALID 

       370        380        390        400        410        420 
HTRPFARSAL RTLAQRKQAH HGLSSLPIIQ AWDRDFYCPP DPPAPPIPLP PLTLGTVFMG 

       430        440        450        460        470        480 
LSRLFRHLYG VSLRPVPSAS GEVWHTDVQK LEVVDEDQGI IGWIYADLFA RRGKASGAAH 

       490        500        510        520        530        540 
YTVRCSRRTD DDDEQGDGMF EGAELQILES QEFEAVKRHR LPNQEGVFQL PLVVLLCEFT 

       550        560        570        580        590        600 
RPTVSKGGTI LEWHEVQTLF HEMGHAMHSM LGRTEYQNVS GTRCATDFVE LPSILMEHFL 

       610        620        630        640        650        660 
NSPAVLSLFD ADGTSTLRQI GNHHHDPCHA IDTYSQIMLA VVDQIYHSPT VLDPSFDSTR 

       670        680        690        700        710        720 
EYGNLQNTRG LIPYVPGTSY QTQFGHLFGY GATYYSYLFD RAIASRVWSK VFSKDPLDRE 

       730        740        750        760        770 
LGEKYKREVL RWGGARDPWE MVATLLDAPE LAAGDAEAMR EVGRWRIEDE VGVGGRH

A8N2T3 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools