NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I., Sieprawska-Lupa M., Whitman W.B., Richardson P.;
"Complete sequence of Methanococcus maripaludis C7.";
Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase (By similarity).
CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
CATALYTIC ACTIVITY: Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates.
SUBUNIT: Heterodimer of a large subunit and a small subunit (By similarity).
SIMILARITY: Belongs to the archaeal DNA polymerase II family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CP000745; ABR66090.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_001330241.1; -.

3D structure databases

ModBase

A6VI14.

Ontologies

GO:0008408;	Molecular function: 3'-5' exonuclease activity (inferred from electronic annotation from HAMAP).
GO:0003677;	Molecular function: DNA binding (inferred from electronic annotation from HAMAP).
GO:0003887;	Molecular function: DNA-directed DNA polymerase activity (inferred from electronic annotation from HAMAP).
GO:0000738;	Biological process: DNA catabolic process, exonucleolytic (inferred from electronic annotation from HAMAP).
GO:0006261;	Biological process: DNA-dependent DNA replication (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00324; -; 1.
PBIL [Tree]

InterPro

IPR004475; PolC_DP2.
IPR016033; PolC_DP2_N.
Graphical view of domain structure.

Pfam

PF03833; PolC_DP2; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF016275; PolC_DP2; 1.

TIGRFAMs

TIGR00354; polC; 1.

BLOCKS

A6VI14.

Genome annotation databases

GeneID

5329159; -.

GenomeReviews

CP000745_GR; MmarC7_1023.

KEGG

mmz:MmarC7_1023; -.

CMR

A6VI14; MmarC7_1023.

Other

ProtoNet

A6VI14.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; DNA replication; DNA-binding; DNA-directed DNA polymerase; Exonuclease; Hydrolase; Multifunctional enzyme; Nuclease; Nucleotidyltransferase; Transferase.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 1131`	`1131`		`DNA polymerase II large subunit.`	`PRO_1000019390`

Sequence information

Length: 1131 AA [This is the length of the unprocessed precursor]

Molecular weight: 128380 Da [This is the MW of the unprocessed precursor]

CRC64: 143EE34A3D4BE0B5 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MLHVSASKGM TEYFKNILDN VTELYTLADD CRKSGYDVTD HVEIPLAKDM ADRVEGIVGP 

        70         80         90        100        110        120 
KNVAERIREL VSDLGKEPAA LEIAKEIVEG KFGEFGREVG AEQAVRTALA VITEGIVAAP 

       130        140        150        160        170        180 
LEGIAHVKIK KNNDSGEYLA IYFAGPIRSA GGTAQALAVL VGDYVRKNMG LDKFKPTEDE 

       190        200        210        220        230        240 
VERYGEEVDL YQSEVTTFQY QPKAEEIRAA VRNISVEITG EATDDVEVSG HRDLPRVETN 

       250        260        270        280        290        300 
QIRGGALLAL VEGVLLKAPK ILRHVDKLGI EGWDWLKELK SKKEEVIEEI EEEKDDFNYE 

       310        320        330        340        350        360 
EEEDLSQYED YEVEAVTKFI GEVIAGRPVF SHPSKKGGFR LRYGRSRNTG FATDGFHPAI 

       370        380        390        400        410        420 
MYLVDDFMAV GTQLKTERPG KATCVVPVDS IEGPIVRLKD KSVLKIDTVE KAKQYRDDVE 

       430        440        450        460        470        480 
EILFLGDILV NYGDFLENNH TILPSSWCTE WYEKILKSEN LEYKKEFIEN PDQKEAVNYA 

       490        500        510        520        530        540 
KLTKTPLHPK YTYFWHDISK DNINVLRNWI IGGKYNESND SWEVTYDSEN PEISNAKRYL 

       550        560        570        580        590        600 
ELIGCPHTVT GEKLEIFEYY PLLYSLGYDF DEKRDIVEEI EEKLQNTKNN MHFINTIAPF 

       610        620        630        640        650        660 
EIRRNAYIYV GARMGRPEKA ASRKMKPPVN GLFPIGNAGA LVRLINKAVE EGKTDEIEIS 

       670        680        690        700        710        720 
NVKCSCGNVS LYRTCPFCGN SVEPSGPSRI KLPIKEYWYK ALENLKINKA GDVKCIKGMT 

       730        740        750        760        770        780 
SKDKIIEPLE KAILRAKNDI YVFKDGTTRF DCTDVPVTHF RPVEIHVGIE KLKSLGYLKD 

       790        800        810        820        830        840 
IHGNSLENED QVLELKVQDV IVPESCMDYF FNVSKFIDDL LEKYYKKDRF YNVNKRDDLV 

       850        860        870        880        890        900 
GHLIIGMAPH TSAGMVGRII GYSNANVGYA HPYFHASKRR NCDGDEDAFF LLLDAFMNFS 

       910        920        930        940        950        960 
KRFLPDKRGG QMDAPLVLTT ILDPKEVDGE VHNMDSMWEY PIEFYEKSLE GIAPKEIKKI 

       970        980        990       1000       1010       1020 
MQTVEDRLDK DSQYEGIGYT HETLKIDEGP LVCAYKTLGS MMEKTSAQLA VAKKIRATDE 

      1030       1040       1050       1060       1070       1080 
RDVAEKVIQS HFVPDLIGNL RAFSRQGVRC KCGAKYRRMP LKGVCRKCGS RLILTVSKGA 

      1090       1100       1110       1120       1130 
VEKYMDVSQT MAEKYEASDY IKQRLEIIRS GIDSLFVNDK RKQVKIEDFF K

A6VI14 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools