|
|
|
|
|
|
[1]
|
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 6260 / CBS 566 / IFO 10279 / JCM 1539 / NRRL Y-324;
Birren B.W.,
Lander E.S.,
Galagan J.E.,
Nusbaum C.,
Devon K.,
Cuomo C.,
Kellis M.,
Rasmussen M.D.,
Grochow J.A.,
Jaffe D.B.,
Butler J.,
Alvarez P.,
Gnerre S.,
Grabherr M.,
Kleber M.,
Mauceli E.W.,
Brockman W.,
MacCallum I.A.,
Rounsley S., ![]()
Young S.K.,
LaButti K.,
Pushparaj V.,
DeCaprio D.,
Crawford M.,
Koehrsen M.,
Engels R.,
Montgomery P.,
Pearson M.,
Howarth C.,
Larson L.,
Luoma S.,
White J.,
Zeng Q.,
Kodira C.D.,
Yandava C.,
Alvarado L.,
O'Leary S.,
Reedy J.,
Heitman J.;
"The genome sequence of Pichia guilliermondii ATCC 6260.";
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
|
[2]
|
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-1219.
DOI=10.1073/pnas.0400938101; PubMed=15070748 [NCBI, ExPASy, EBI, Israel, Japan]
Liu Y.J.,
Hall B.D.;
"Body plan evolution of ascomycetes, as inferred from an RNA polymerase II phylogeny.";
Proc. Natl. Acad. Sci. U.S.A. 101:4507-4512(2004).
|
[3]
|
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 398-766, AND VARIANTS ALA-401; VAL-519; THR-722 AND LYS-727.
STRAIN=ATCC 46036 / CBS 2030 / IFO 10106 / NRRL Y-2075, MMRL 1635, MMRL 1636, and MMRL 1759;
DOI=10.1128/JCM.42.12.5624-5635.2004; PubMed=15583292 [NCBI, ExPASy, EBI, Israel, Japan]
Diezmann S.,
Cox C.J.,
Schoenian G.,
Vilgalys R.J.,
Mitchell T.G.;
"Phylogeny and evolution of medical species of Candida and related taxa: a multigenic analysis.";
J. Clin. Microbiol. 42:5624-5635(2004).
|
|
|
|
- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template (By similarity).
- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
- SUBUNIT: Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits (By similarity).
- SUBCELLULAR LOCATION: Nucleus (By similarity).
- MISCELLANEOUS: The binding of ribonucleoside triphosphate to the RNA polymerase II transcribing complex probably involves a two-step mechanism. The initial binding seems to occur at the entry (E) site and involves a magnesium ion coordinated by three conserved aspartate residues of the two largest RNA Pol II subunits (By similarity).
- SIMILARITY: Belongs to the RNA polymerase beta chain family.
- SEQUENCE CAUTION:
- Sequence=AAT47730.1; Type=Frameshift; Positions=594, 601;
|
|
|
|
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| Length: 1236 AA [This is the length of the unprocessed precursor] |
Molecular weight: 140194 Da [This is the MW of the unprocessed precursor] |
CRC64: 31F6C07ECFA7C2CD [This is a checksum on the sequence] |
|
10 20 30 40 50 60
MSDQASDPYM YDDDSLSITP EDCWTVISSF FQEKGLVSQQ LDSFDEFIES TIQELVWEDS
70 80 90 100 110 120
RLILDQPAQH TSEEDHENRR YEITFGKIYI SKPTQTEGDG TTHPMFPQEA RLRNLTYSSP
130 140 150 160 170 180
LYVDMTKRVL KSDDNAGNEH ELEWIEEEIK DEEPLTKVYL GKVPIMLRSK FCMLRDLGEH
190 200 210 220 230 240
EFYELKECPY DMGGYFVING SEKVLIAQER SAANIVQVFK KAAPSPISHV AEIRSALERG
250 260 270 280 290 300
SRLISSMQIK LYGREEKSTS NRTIKATLPY IKEDIPIVIV FRALGIVPDG DILEHICYDA
310 320 330 340 350 360
NDWQMLEMLK PCVEEGFVIQ EREVALDFIG RRGALGIKRE KRIQYAKDIL QKELLPNITQ
370 380 390 400 410 420
DEGFETRKAF FLGYMVNRLL LCALERKEPD DRDHFGKKRL DLAGPLLASL FRILFKKLTK
430 440 450 460 470 480
DIYNYMQRCV ENDKVFNLTL AVKSQTITDG LRYSLATGNW GEQKKAMSSR AGVSQVLNRY
490 500 510 520 530 540
TYSSTLSHLR RTNTPIGRDG KIAKPRQLHN THWGLVCPAE TPEGQACGLV KNLSLMSCIS
550 560 570 580 590 600
VGTPSEPILY FLEEWGMEPL EDYVPSNSPD STRVFVNGVW VGTHREPAHL VDTMRSLRRR
610 620 630 640 650 660
GDISPEVSII RDIREKEFKI FTDAGRVYRP LFIVDDDPES ETKGELKLQK EHIHKLLNAE
670 680 690 700 710 720
YSEEYATNEF GEEEGPYGWS SLVNDGVVEY VDAEEEETIM IAMTPEDLEA SKSSLTATQQ
730 740 750 760 770 780
KSLQLEEQEL DPAKRIKPTN SSTTSTFTHC EIHPSMILGV AASIIPFPDH NQSPRNTYQS
790 800 810 820 830 840
AMGKQAMGVF LTNYSVRMDT MANILYYPQK PLATTRAMEH LKFRELPAGQ NAIVAIACYS
850 860 870 880 890 900
GYNQEDSMIM NQSSIDRGLF RSLFFRTYMD LEKRQGMKAL ETFEKPLRSD TLRLKHGTYE
910 920 930 940 950 960
KLDDDGLIAP GIRVSGEDII IGKTTPIPPD TEELGQRTQY HTKRDASTPL RSTESGIVDQ
970 980 990 1000 1010 1020
VLLTTNGDGA KFVKVRMRTT KVPQIGDKFA SRHGQKGTVG VTYRHEDMPF TSQGIVPDLI
1030 1040 1050 1060 1070 1080
INPHAIPSRM TVAHLIECLL SKVSSLSGLE GDASPFTDVT AEAVSKLLRE HGYQSRGFEV
1090 1100 1110 1120 1130 1140
MYHGHTGKKL MAQVFFGPTY YQRLRHMVDD KIHARARGPV QVLTRQPVEG RSRDGGLRFG
1150 1160 1170 1180 1190 1200
EMERDCMIAH GAAGFLKERL MEASDAFRVH VCGVCGLMSV IANLKKNQFE CRSCKNKTNI
1210 1220 1230
YQIHIPYAAK LLFQELMAMN ISPRLYTERS GVSVRT
|
A5DHT2 in FASTA format |
|
ExPASy Home page
APAF Australia
