RNA-directed RNA polymerase
     (EC 2.7.7.48)
mRNA (guanine-N(7)-)-methyltransferase
     (EC 2.1.1.56)
mRNA guanylyltransferase
     (EC 2.7.7.-)

Gene name

Name:

From

European bat lyssavirus 1 (strain Bat/Germany/RV9/1968) (EBLV1)

[TaxID: 453115]

Taxonomy

Viruses; ssRNA negative-strand viruses; Mononegavirales; Rhabdoviridae; Lyssavirus.

Virus host

Mammalia [TaxID: 40674]

Protein existence

3: Inferred from homology;

References

[1]

NUCLEOTIDE SEQUENCE [GENOMIC RNA].
DOI=10.1099/vir.0.82692-0; PubMed=17374776 [NCBI, ExPASy, EBI, Israel, Japan]
Marston D.A., McElhinney L.M., Johnson N., Muller T., Conzelmann K.K., Tordo N., Fooks A.R.;
"Comparative analysis of the full genome sequence of European bat lyssavirus type 1 and type 2 with other lyssaviruses and evidence for a conserved transcription termination and polyadenylation motif in the G-L 3' non-translated region.";
J. Gen. Virol. 88:1302-1314(2007).

Comments

FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently five subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals (By similarity).
CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m₇G(5')pppR-RNA.
SUBUNIT: Interacts with the P protein (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
SIMILARITY: Belongs to the rhabdoviridae protein L family.
SIMILARITY: Contains 1 RdRp catalytic domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

EF157976; ABO65247.1; -; Genomic_RNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_001285392.1; -.

3D structure databases

ModBase

A4UHQ2.

Family and domain databases

InterPro

IPR017234; RNA-dir_RNA_pol_rhabdovir.
IPR014023; RNA_pol_cat.
IPR001016; RNA_pol_L_viral.
Graphical view of domain structure.

Pfam

PF00946; Paramyx_RNA_pol; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF037546; RNA_pol_RhabdoV_sub; 1.

PROSITE

PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

A4UHQ2.

Genome annotation databases

GeneID

5219906; -.

Other

ProtoNet

A4UHQ2.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; Complete proteome; Cytoplasm; Methyltransferase; mRNA capping; mRNA processing; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase; Virion.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 2127`	`2127`	`Large structural protein.`	`PRO_0000297834`
`DOMAIN`	`611 799`	`189`	`RdRp catalytic.`

Sequence information

Length: 2127 AA [This is the length of the unprocessed precursor]

Molecular weight: 242548 Da [This is the MW of the unprocessed precursor]

CRC64: D26808658BFD089E [This is a checksum on the sequence]

        10         20         30         40         50         60 
MIDSTEVYDD PVDPVEPEAD LRSNSVVPNI LRNSDYNLNS PLLEDPSRLM IEWLTTGNRP 

        70         80         90        100        110        120 
SRLNLTDNLL RSYKVLRGYF KKLDVGSMRA GGLGAQAMIT LWLHGSHSES TRSRKCLTEL 

       130        140        150        160        170        180 
SQFYKKSSPI EKLLNYTLEN RGLKTPTEGV LSSINKVQYD QAFGRYLGNT YSSYLFFHVI 

       190        200        210        220        230        240 
ILYMNALDWD EEKTILALWK EIASIDVKSD LVKFRDQIWG SLVITKDFVY SQSANCLFDR 

       250        260        270        280        290        300 
NYTLMLKDLF LSRFNSLLIL ISPPESRYSD DLVSNLCQLY IAGDKVLSAC GNSGYDVIKL 

       310        320        330        340        350        360 
LEPYIVNKLV QKAEKFRPLI HSLGDFPQFI RDKTNQLEGT FGPSAREFFQ TMDLLDNIHD 

       370        380        390        400        410        420 
LVFVYGCYRH WGHPYIDYRK GLSKLYDQVH VKKIIDRNYQ ECLASDLAKR ILRWGFDKYS 

       430        440        450        460        470        480 
RWYLDSNLLP GDHPLSPYVK TQTWPPKHVV DMVGDTWHSL PITQIFEIPE SMDPSEILDD 

       490        500        510        520        530        540 
KSHSFTRTRL ASWLSENRGG PVPSEKVIIT ALSRPPVNPR EFLRSIDVGG LPDDDLIIGL 

       550        560        570        580        590        600 
KPKERELKIE GRFFALMSWN LRLYFVITEK LLATYILPLF DALTMTDNLN KVFKKLIDRV 

       610        620        630        640        650        660 
TGQGLLDYSR VTYAFHLDYE KWNNHQRLES TKDVFSVLDK VFGLKKVFSR THEFFQKSWV 

       670        680        690        700        710        720 
YYSDRSDLIG LWRDQIYCLD MTNGPTCWNG QDGGLEGLRQ KGWSLVSLLM IDRESQTRNT 

       730        740        750        760        770        780 
RTKILAQGDN QVLCPTYMLS SGLNKEGLLY ELDSISRNAI SIYRAIEDGA SKLGLIIKKE 

       790        800        810        820        830        840 
ETMCSYDFLI YGKTPLFRGN ILVPESKRWA RVSCISNDQI VNLANIMSTV STNALTVAQH 

       850        860        870        880        890        900 
SQSLIKPMRD FLLMSVQAVY HYLLFSPILK DRVYKILVAD GTEFLLAMSR IIYLDPSLGG 

       910        920        930        940        950        960 
VSGMSLGRFH IRQFSDPVSE GLSFWREIWL SSSESWIHAL CQEAGNPDLG DRSLESFTRL 

       970        980        990       1000       1010       1020 
LEDPTTLNIR GGASPTILLR EAIRKALYDE VDKVENSEFR EAILLSKTHR DNFILFLRSV 

      1030       1040       1050       1060       1070       1080 
EPLFPRFLSE LFSSSFLGIP ESIIGLIQNS RTIRRQFRRS LSRTLEESFF NSEIHGISRM 

      1090       1100       1110       1120       1130       1140 
TQVPQRIGRV WSCSAERADQ LREISWGRKV VGTTVPHPSE MLALIPKSSI SCTCGQSGDD 

      1150       1160       1170       1180       1190       1200 
SPRISVSVLP SLDQSFFSRG PLKGYLGSST SMSTQLFHAW EKVTNVHVVK RALSLKESIN 

      1210       1220       1230       1240       1250       1260 
WFITRDSNLA QTLIRNIVSL TGPFFPLEEA PVFKRTGSAL HRFKSARYSE GGYSSICPNL 

      1270       1280       1290       1300       1310       1320 
LSHISVSTDT MSDLTQDGTN FDFMFQPLML YAQTWTSELV QKDLRLRDST FHWHLRCLKC 

      1330       1340       1350       1360       1370       1380 
IRPIDDITLE APKIFSFPDV SKRISRMVSG AVPQFRKLPE IGLKPGKFDS LKEKDKSRHI 

      1390       1400       1410       1420       1430       1440 
GTAQGLLYSI LVATHDSGYN DGTIFPVNIY GKVSPRDYLR GLARGVLIGS SICFLTRMTN 

      1450       1460       1470       1480       1490       1500 
ININRPLELI SGVISYILLR LDNHPSLYVM LKEPSLRSEI FSIPQKIPAA YPTTMKEGNR 

      1510       1520       1530       1540       1550       1560 
SVLCYLQHVL RYEREAITTS PENDWLWIFS DFRSIKMTYL TLITYQSHLL LQRIDRNLSK 

      1570       1580       1590       1600       1610       1620 
QMRVRLRQLN SLMRQVLGGH GEGTLDSDED IQGLLRDALQ RTRWVDQEVR HAAKTMKCDY 

      1630       1640       1650       1660       1670       1680 
SPSKRVSRKA GCSEWICSAQ QVAVSTSSNP APISEIDIRA LSKKLQNPLI SGLRVVQWAT 

      1690       1700       1710       1720       1730       1740 
GAHYKLKPIL NDLEAYPTLC LVVGDGSGGI SRAVLSMFPD AKLVFNSLLE VNDLMASGTH 

      1750       1760       1770       1780       1790       1800 
PLPPSALMSG GEDIVSRVID FNSIWEKPSD LRNPSTWRYF QTVQSAANMS YDLIICDAEV 

      1810       1820       1830       1840       1850       1860 
TDIPSINKIT LLMSDFSLSI NGPLSLIFKT YGTMLVNPDY KAVQHLSRAF PSVTGYITQM 

      1870       1880       1890       1900       1910       1920 
TSSFSSELYL KFSKRGKFFR DSEYLTSSTL REMSLVLFNC SSSKSEMLRA RSLNYQDLVR 

      1930       1940       1950       1960       1970       1980 
GFPEEIISNP YNEMIITLID NDVESFLVHK MVDDLELRQG AFSKMSIILT IMMVFSNRVF 

      1990       2000       2010       2020       2030       2040 
NVSKPLNDPK FYPPSDPKIL RHFNICCGTL IYLSAALGDV LNFARLHELY NNPVTYYFKK 

      2050       2060       2070       2080       2090       2100 
QTLGGRMYLA WSWTDNTPVF KRVACNSSLS LSSHWIRLIY KIVKTTRLVG SAKDLSQEVE 

      2110       2120 
KHLKSYNRWI NFSDLRSRSS LLDYSCL

A4UHQ2 in FASTA format

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View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools