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UniProtKB/Swiss-Prot entry A3RM23

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name L_RABVI
Primary accession number A3RM23
Secondary accession number Q58FH0
Integrated into Swiss-Prot on July 10, 2007
Sequence was last modified on April 3, 2007 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 7)
Name and origin of the protein
Protein name Large structural protein
Synonyms Protein L
Transcriptase
Replicase
Includes RNA-directed RNA polymerase
     (EC 2.7.7.48)
mRNA (guanine-N(7)-)-methyltransferase
     (EC 2.1.1.56)
mRNA guanylyltransferase
     (EC 2.7.7.-)
Gene name
Name: L
From
Rabies virus (strain India) (RABV) [TaxID: 445790] 
Taxonomy Viruses; ssRNA negative-strand viruses; Mononegavirales; Rhabdoviridae; Lyssavirus.
Virus host Mammalia [TaxID: 40674]
Protein existence 3: Inferred from homology;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC RNA].
STRAIN=Isolate Germany;
Pfefferle S., Panning M., Drosten C.;
"Virological characterization of cases of transplantation-associated Rabies in Germany.";
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
[2]
 NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Desai A., Nagaraja T., Muhamuda K., Madhusudana S., Ravi V.;
"Complete nucleotide sequencing of an Indian isolate of Rabies virus.";
Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
Comments
  • FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-7-N-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently five subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals (By similarity).
  • CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
  • CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
  • SUBUNIT: Interacts with the P protein to form the functional polymerase (By similarity).
  • SUBCELLULAR LOCATION: Virion. Cytoplasm (By similarity).
  • SIMILARITY: Belongs to the rhabdoviruses protein L family.
  • SIMILARITY: Contains 1 RdRp catalytic domain.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AY956319; AAX56085.1; -; Genomic_RNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
EF437215; ABO15580.1; -; Genomic_RNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
3D structure databases
ModBase A3RM23.
Ontologies
GO
GO:0004482; Molecular function: mRNA (guanine-N7-)-methyltransferase activity (inferred from electronic annotation from EC).
QuickGo view.
Family and domain databases
InterPro IPR017234; RNA-dir_RNA_pol_rhabdovir.
IPR014023; RNA_pol_cat.
IPR001016; RNA_pol_L_viral.
Graphical view of domain structure.
Pfam PF00946; Paramyx_RNA_pol; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF037546; RNA_pol_RhabdoV_sub; 1.
PROSITE PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS A3RM23.
Other
ProtoNet A3RM23.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
ATP-binding; Complete proteome; Cytoplasm; Methyltransferase; mRNA capping; mRNA processing; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase; Virion.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom    To Length Description FTId
CHAIN   1   2127  2127     Large structural protein. PRO_0000294422
DOMAIN   611    799  189     RdRp catalytic. 
REGION   1562   2127  566     Interaction with P protein (By similarity). 
VARIANT   93     93  1     T -> A (in strain: Isolate Germany). 
VARIANT   232    232  1     H -> Q (in strain: Isolate Germany). 
VARIANT   361    361  1     L -> V (in strain: Isolate Germany). 
VARIANT   471    471  1     P -> S (in strain: Isolate Germany). 
VARIANT   488    488  1     A -> T (in strain: Isolate Germany). 
VARIANT   502    502  1     A -> V (in strain: Isolate Germany). 
VARIANT   525    525  1     T -> S (in strain: Isolate Germany). 
VARIANT   966    966  1     T -> A (in strain: Isolate Germany). 
VARIANT   1373   1373  1     G -> D (in strain: Isolate Germany). 
VARIANT   1825   1825  1     Y -> C (in strain: Isolate Germany). 
VARIANT   1841   1841  1     K -> N (in strain: Isolate Germany). 
VARIANT   1845   1845  1     H -> Q (in strain: Isolate Germany). 
VARIANT   1872   1872  1     F -> C (in strain: Isolate Germany). 
VARIANT   1904   1904  1     R -> K (in strain: Isolate Germany). 
VARIANT   1960   1960  1     R -> G (in strain: Isolate Germany). 
VARIANT   2091   2091  1     S -> N (in strain: Isolate Germany). 
Sequence information
Length: 2127 AA [This is the length of the unprocessed precursor] Molecular weight: 242770 Da [This is the MW of the unprocessed precursor] CRC64: 55202B0E620305C8 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MLDPGEVYDD PVDPIESDAE PRGAPTVPNI LRNSDYNLNS PLIEDPARLM LEWLTTGNRP 

        70         80         90        100        110        120 
YRMTLTDNCS RSYKVLKDYF KKVDLGSLKV GGTAAQSMIS LWLYGAHSES NRSRKCITEL 

       130        140        150        160        170        180 
AHFYSKSSPI EKLLNCTLGN RGLRIPPEGV LSCLERVDYD KAFGRYLANT YSSYLFFHVI 

       190        200        210        220        230        240 
TLYMNALDWD EEKTILALWK ELTSVDIGKD LVKFKDQIWG LLIVTKDFVY SHSSNCLFDR 

       250        260        270        280        290        300 
NYTLMLKDLF LSRFNSLMIL LSPPEPRYSD DLISQLCQLY IAGDQVLSMC GNSGYEVIKI 

       310        320        330        340        350        360 
LEPYVVNSLV QRAERFRPLI HSLGDFPVFI KDKVSQLEGT FGPSAKRFFG VLDQFDNIHD 

       370        380        390        400        410        420 
LVFVYGCYRH WGHPYIDYRK GLSKLYDQVH IKKVIDKSYQ ECLASDLARR ILRWGFDKYS 

       430        440        450        460        470        480 
KWYLDSRLLT RDHPLTPYIK TQTWPPKHIV DLVGDTWHKL PITQIFEIPE PMDPSEILDD 

       490        500        510        520        530        540 
KSHSFTRARL ASWLSENRGG PAPSEKVIIT ALSKPPVNPR EFLKTIDLGG LPDEDLIIGL 

       550        560        570        580        590        600 
KPKERELKIE GRFFALMSWN LRLYFVITEK LLANYILPLF DALTMTDNLN KVFKKLIDRV 

       610        620        630        640        650        660 
TGQGLLDYSR VTYAFHLDYE KWNNHQRLES TEDVFSVLDH VFGLKRVFSR THEFFQKSWI 

       670        680        690        700        710        720 
YYSDRSDLIG LWEDQIYCLD MSNGPTCWNG QDGGLEGLRQ KGWSLVSLLM IDRESQTRNT 

       730        740        750        760        770        780 
RTKILAQGDN QVLCPTYMLS PGLSREGLLY ELESISRNAL SIYRAIEEGA SKLGLIIKKE 

       790        800        810        820        830        840 
ETMCSYDFLI YGKTPLFRGN ILVPESKRWA RVSCISNDQI VNLANIMSTV STNALTVAQH 

       850        860        870        880        890        900 
SQSLIKPMRD FLLMSVQAVF HYLLFSPILK GRVYKILSAE GESFLLAMSR IIYLDPSLGG 

       910        920        930        940        950        960 
VSGMSLGRFH IRQFSDPVSE GLSFWREIWL SSHESWIHAL CQEAGNPDLG ERTLESFTRL 

       970        980        990       1000       1010       1020 
LEDPTTLNIK GGASPTILLK DAIRKALYDE VDKVENSEFR EAILLSKTHR DNFILFLKSV 

      1030       1040       1050       1060       1070       1080 
EPLFPRFLSE LFSSSFLGIP ESIIGLIQNS RTIRRQFRRS LSRTLEESFY NSEIHGINRM 

      1090       1100       1110       1120       1130       1140 
TQTPQRVGRV WPCSSERADL LREISWGRKV VGTTVPHPSE MLGLLPKSSI SCPCGATGGG 

      1150       1160       1170       1180       1190       1200 
NPRVSVSVLP SFDQSFFSRG PLKGYLGSST SMSTQLFHAW EKVTNVHVVK RALSLKESIN 

      1210       1220       1230       1240       1250       1260 
WFITRNSNLA QTLIRNIMSL TGPDFPLEEA PVFKRTGSAL HRFKSARYSE GGYSSVCPNL 

      1270       1280       1290       1300       1310       1320 
LSHISVSTDT MSDLTQDGKN YDFMFQPLML YAQTWTSELV QKDTRLRDST FHWHLRCNRC 

      1330       1340       1350       1360       1370       1380 
VRPIDDITLE TSQIFEFPDV SKRISRMVSG AVPHFQKLPD IRLKPGDFES LSGREKSRHI 

      1390       1400       1410       1420       1430       1440 
GSAQGLLYSI LVAIHDSGYN DGTIFPVNIY GKVSPRDYLR GLARGVLIGS SICFLTRMTN 

      1450       1460       1470       1480       1490       1500 
ININRPLELI SGVISYILLR LDNHPSLYIM LREPSLRGEI FSIPQKIPAA YPTTMKEGNR 

      1510       1520       1530       1540       1550       1560 
SILCYLQHVL RYEREVITAS PENDWLWIFS DFRSAKMTYL TLITYQSHLL LQRVERNLSK 

      1570       1580       1590       1600       1610       1620 
SMRANLRQMS SLMRQVLGGH GEDTLESDDD VQRLLKDSLR RTRWVDQEVR HAARTMTGDY 

      1630       1640       1650       1660       1670       1680 
SPNKKLSRKA GGSEWVCSAQ QVAVSTSANP APVLELDIRA LSKRFQNPLI SGLRVVQWAT 

      1690       1700       1710       1720       1730       1740 
GAHYKLKPIL DDLNVFPSLC LVVGDGSGGI SRAVLNMFPD AKLVFNSLLE VNDLMASGTH 

      1750       1760       1770       1780       1790       1800 
PLPPSAIMSG GDDIVSRVID FDSIWEKPSD LRNLTTWKYF QSVQKQVNMS YDLIICDAEV 

      1810       1820       1830       1840       1850       1860 
TDIASINRIT LLMSDFALSI DGPLYLVFKT YGTMLVNPDY KAIQHLSRAF PSVTGFITQV 

      1870       1880       1890       1900       1910       1920 
TSSFSSELYL RFSKRGKFFR DAEYLTSSTL REMSLVLFNC SSPRSEMQRA RSLNYQDLVR 

      1930       1940       1950       1960       1970       1980 
GFPEEIISNP YNEMIITLID SDVESFLVHK MVDDLELQRR TLSKVAIIIA IMIVFSNRVF 

      1990       2000       2010       2020       2030       2040 
NVSKPLTDPL FYPPSDPKIL RHFNICCSTM MYLSTALGDV PSFARLHDLY NRPITYYFRK 

      2050       2060       2070       2080       2090       2100 
QVIRGNIYLS WSWSDDTAVF KRVACNSSLS LSSHWIRLIY KIVKTTRLVG SIEDLSGEIE 

      2110       2120 
RHLRGYNRWI TLEDIRCRSS LLDYSCL
A3RM23 in FASTA format

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