FUNCTION: Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase (By similarity).
CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
CATALYTIC ACTIVITY: Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates.
SUBUNIT: Heterodimer of a large subunit and a small subunit (By similarity).
PTM: This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation (Potential).
SIMILARITY: Belongs to the archaeal DNA polymerase II family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CP000562; ABN58047.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_001048029.1; -.

3D structure databases

ModBase

A3CXE7.

Ontologies

GO:0008408;	Molecular function: 3'-5' exonuclease activity (inferred from electronic annotation from HAMAP).
GO:0003677;	Molecular function: DNA binding (inferred from electronic annotation from HAMAP).
GO:0003887;	Molecular function: DNA-directed DNA polymerase activity (inferred from electronic annotation from HAMAP).
GO:0000738;	Biological process: DNA catabolic process, exonucleolytic (inferred from electronic annotation from HAMAP).
GO:0006261;	Biological process: DNA-dependent DNA replication (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00324; -; 1.
PBIL [Tree]

InterPro

IPR003586; Hedgehog_hint_C.
IPR003587; Hedgehog_hint_N.
IPR006141; Intein_splicing_site.
IPR004475; PolC_DP2.
IPR016033; PolC_DP2_N.
Graphical view of domain structure.

Pfam

PF03833; PolC_DP2; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF016275; PolC_DP2; 1.

SMART

SM00305; HintC; 1.
SM00306; HintN; 1.
SMART graphical view of domain structure.

TIGRFAMs

TIGR01443; intein_Cterm; 1.
TIGR01445; intein_Nterm; 1.
TIGR00354; polC; 1.

PROSITE

PS50818; INTEIN_C_TER; 1.
PS50817; INTEIN_N_TER; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

A3CXE7.

Genome annotation databases

GeneID

4847324; -.

GenomeReviews

CP000562_GR; Memar_2124.

KEGG

mem:Memar_2124; -.

CMR

A3CXE7; Memar_2124.

Other

ProtoNet

A3CXE7.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Autocatalytic cleavage; Complete proteome; DNA replication; DNA-binding; DNA-directed DNA polymerase; Exonuclease; Hydrolase; Multifunctional enzyme; Nuclease; Nucleotidyltransferase; Protein splicing; Transferase.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 870`	`870`	`DNA polymerase II large subunit, 1st part (Potential).`	`PRO_0000294688`
`CHAIN`	`871 1035`	`165`	`Memar polC intein (Potential).`	`PRO_0000294689`
`CHAIN`	`1036 1285`	`250`	`DNA polymerase II large subunit, 2nd part (Potential).`	`PRO_0000294690`

Sequence information

Length: 1285 AA [This is the length of the unprocessed precursor]

Molecular weight: 142303 Da [This is the MW of the unprocessed precursor]

CRC64: 7DF1F55811763A22 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MEVSPAIARY FEELEGELDA AIRLAAAARA RGLDPRTEIE IPVASDLADR VEALLGYKGI 

        70         80         90        100        110        120 
AARIRELEAE MSREEAALRI GDDFAARKFG ETTPEEILDH AIRAAMALLT EGVVAAPTEG 

       130        140        150        160        170        180 
IGKVSLGKND DGTDYLKIYY AGPIRSAGGT AQALSVLVGD YVRQALGINR YIPRPEEVER 

       190        200        210        220        230        240 
YIEEIRQYNN IMSLQYLPSE KELRMIIENC PVCIDGEPTE QQEVSGYRNL ERVETNTVRG 

       250        260        270        280        290        300 
GMALVVAEGL ALKAPKVLKN VRKMKMEGWD WIEEMIGGGP KSDDDDASAA IKPKDKYIRD 

       310        320        330        340        350        360 
LIGGRPVFSY PMRKGGFRLR LGRARNTGFA AAGFNPATLH ILGDFLAVGT QMKVERPGKA 

       370        380        390        400        410        420 
AGVVPVDSIQ GPTVKLRSGE VRRVDDAAEA RRLAGQVDEI LDVGEMLVSF GEFMENNHPL 

       430        440        450        460        470        480 
MPPAYCEEWW MLEGGPRHPE NELEAIEFAL DGVPLHPDYT YMWDDVAPAD IARLAEAVGT 

       490        500        510        520        530        540 
GGTVEDGVLM IRNTPETKAI LEELLIPHHL SGDRLAIREH LAFLACLGLT LQLTKRPAWQ 

       550        560        570        580        590        600 
DAPMENSLDL VMHLSGFTVR SRAGTRIGGR MGRPGKSKPR EMRPPPHSLF PIGDEGGARR 

       610        620        630        640        650        660 
SFQAACSSKP RSNTDGGVIE AEVGERQCPA CGAFTYKNLC ECGAHTNPVF RCPRCGKDVG 

       670        680        690        700        710        720 
QDVCPRCNAE TVCLQKVTIN VKAEYLAAME SLGVRESSVA LLKGVKGLIS RERPVEPIEK 

       730        740        750        760        770        780 
GILRALQNLY VFKDGTVRYD MIDLPLTHFR PDEVGVPIER LRELGYTHDT YGRELVSDDQ 

       790        800        810        820        830        840 
VLELRHQDIL VSEGCGEWLV RVAKFVDDLL VRLYGLEPFY KAEKPLDLVG HLLMGLAPHT 

       850        860        870        880        890        900 
SAGVLVRLIG FSKAPVGYGH PFFHAAKRRN CFAGDTEITV SDGRRWMSLP IRQFVTENFD 

       910        920        930        940        950        960 
ISKPGLDHVG TFYSDPRQPF YVRSIDSQGK TSLKRVTSVS VHRAPAHLVR FATRRGRVLT 

       970        980        990       1000       1010       1020 
VTPDHAMLVW DTDYLRKIKA LEVAVGDRVP VEEGGLVVAD EVVSRETVQA LDDRVYCLTV 

      1030       1040       1050       1060       1070       1080 
AENHTLVANG IFCGQCDGDE DCVMLLLDGL INFSRAYLPE TRGGTMDAPL VLTTRIDPSE 

      1090       1100       1110       1120       1130       1140 
VDKESHNVDV CDHYPIEVYN GCLAYAHPKD LDAFVDRVER RLGTPAQCEG FLFTHQTSNI 

      1150       1160       1170       1180       1190       1200 
SAGPLESTYT RLGSMLDKLE AELDLAKRIR AVDEDDVAER VLNTHFIRDL QGNLNAFSKQ 

      1210       1220       1230       1240       1250       1260 
KVRCMKCNAK YRRMPLAGKC TRCGGHVIPT VHEGSVKKYL EMSRNICATY AISDYTKQRV 

      1270       1280 
EVLFMQIEST FGEPPEKQLG LADFM

A3CXE7 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools