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| Kingdom | Residues | Position | Rule |
| Eukaryotes | C | N-terminus after cleavage of signal peptide. | Associated with cholesterol glycine ester |
| Eukaryotes | C | C-terminal part of mature peptide. | - |
| Eukaryotes | S,T | - | - |
| Eukaryotes | K | anywhere | - |
| Prokaryotes | C | N-terminus after cleavage of signal peptide. | Frequently associated with S-diacylglycerol cystein |
| Prokaryotes | K | anywhere | - |
| average | 238.4136 |
| monoisotopic | 238.2297 |
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