Sample Preparation and Post-separation Analysis

P38489

General information about the entry
View entry in simple text format
Entry name	NFNB_ECOLI
Primary accession number	P38489
integrated into SWISS-2DPAGE on	October 1, 1998 (release 8)
2D Annotations were last modified on	March 31, 2004 (version 4)
General Annotations were last modified on	November 14, 2006 (version 11)
Name and origin of the protein
Description	Oxygen-insensitive NAD(P)H nitroreductase (EC 1.-.-.-) (FMN-dependent nitroreductase) (Dihydropteridine reductase) (EC 1.5.1.34).
Gene name	Name=nfnB Synonyms=dprA, nfsB, nfsI, ntr OrderedLocusNames=b0578, JW0567
Annotated species	Escherichia coli [TaxID: 562]
Taxonomy	Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan] Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C. ''New perspectives in the Escherichia coli proteome investigation'' Proteomics 1:409-423(2001) [2]   MAPPING ON GEL PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan] Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S. ''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'' Proteomics 2:1682-1698(2002)
2D PAGE maps for identified proteins
How to interpret a protein
ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)} Escherichia coli   map experimental info   protein estimated location		ECOLI-DIGE4.5-6.5 MAP LOCATIONS: SPOT 2D-001WU3: pI=5.83; Mw=24964   [identification data] MAPPING (identification): Peptide mass fingerprinting and tandem mass spectrometry [2].
ECOLI4.5-5.5 {Escherichia coli(4.5-5.5)} Escherichia coli   map experimental info   protein estimated location		ECOLI4.5-5.5 MAP LOCATIONS: SPOT 2D-001HEN: pI=5.55; Mw=25092   [identification data] SPOT 2D-0015EU: pI=5.09; Mw=14160   [identification data] MAPPING (identification): Peptide mass fingerprinting [1].
ECOLI5-6 {Escherichia coli(5-6)} Escherichia coli   map experimental info   protein estimated location		ECOLI5-6 MAP LOCATIONS: SPOT 2D-001LGL: pI=5.47; Mw=32502   [identification data] SPOT 2D-001LKH: pI=5.32; Mw=28018   [identification data] MAPPING (identification): Peptide mass fingerprinting [1].
Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://www.expasy.org/ch2d/license.html or send email to license@isb-sib.ch).
Cross-references
UniProtKB/Swiss-Prot	P38489; NFNB_ECOLI.
2D PAGE maps for unidentified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.
Arabidopsis thaliana (Mouse-ear cress)	Arabidopsis thaliana
Dictyostelium discoideum (Slime mold)	Dictyostelium discoideum
Escherichia coli	Escherichia coli Escherichia coli(4-5) Escherichia coli(5.5-6.7) Escherichia coli(6-11) Escherichia coli(6-9)
Homo sapiens (Human)	Colorectal epithelia cells Cerebrospinal Fluid Colorectal adenocarcinoma cell line (DL-1) Erythroleukemia Cell HepG2 Secreted Proteins HepG2 Promyelocytic leukemia cells Kidney Liver Lymphocytes Lymphoma Soluble nuclear proteins and matrix from liver tissue SDS-PAGE of nucleolar proteins from Human HeLa cells 2D-PAGE of nucleolar proteins from Human HeLa cells Plasma Platelet Red blood cells Macrophage Like Cell Line
Mus musculus (Mouse)	Brown adipose tissue Pancreatic islet cells Liver Gastrocnemius muscle Soluble nuclear proteins and matrix from liver tissue White adipose tissue
Saccharomyces cerevisiae (Baker's yeast)	Saccharomyces cerevisiae
Staphylococcus aureus (strain N315)	Staphylococcus aureus