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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P04406




SWISS-2DPAGE:  P04406


P04406


General information about the entry
View entry in simple text format
Entry nameG3P_HUMAN
Primary accession numberP04406
integrated into SWISS-2DPAGE on August 1, 1993 (release 0)
2D Annotations were last modified onMarch 31, 2004 (version 1)
General Annotations were last modified on January 31, 2008 (version 15)
Name and origin of the protein
DescriptionGlyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) (GAPDH).
Gene nameName=GAPDH
Synonyms=GAPD
ORFNames=CDABP0047, OK/SW-cl.12
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
MEDLINE=94147969; PubMed=8313870; [NCBI, ExPASy, EBI, Israel, Japan]
Hughes G.J., Frutiger S., Paquet N., Pasquali C., Sanchez J.-C., Tissot J.-D., Bairoch A., Appel R.D., Hochstrasser D.F.
''Human liver protein map: update 1993''
Electrophoresis 14:1216-1222(1993)
[2]   MAPPING ON GEL
MEDLINE=93120196; PubMed=1477117; [NCBI, ExPASy, EBI, Israel, Japan]
Golaz O., Walzer C., Hochstrasser D.F., Bjellqvist B., Turler H., Balant L.P.
''Red blood cell protein map: a comparison between carrier-ampholyte pH gradient and immobilized pH gradient, and identification of four red blood cell enzymes''
Appl. Theor. Electrophor. 3:77-82(1992)
[3]   MAPPING ON GEL
MEDLINE=96007934; PubMed=7498157; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch A., Hochstrasser D.F.
''Inside SWISS-2DPAGE database''
Electrophoresis 16:1131-1151(1995)
[4]   MAPPING ON GEL
MEDLINE=96007935; PubMed=7498158; [NCBI, ExPASy, EBI, Israel, Japan]
Gravel P., Sanchez J.-C., Walzer C., Golaz O., Hochstrasser D.F., Balant L.P., Hughes G.J., Garcia-Sevilla J., Guimon J.
''Human blood platelet protein map established by two-dimensional polyacrylamide gel electrophoresis''
Electrophoresis 16:1152-1159(1995)
Comments
  • SUBUNIT: HOMOTETRAMER
2D PAGE maps for identified proteins
How to interpret a protein

LIVER_HUMAN {Liver}
Homo sapiens (Human)
Tissue: Liver
LIVER_HUMAN
  map experimental info
  protein estimated location
 
LIVER_HUMAN

MAP LOCATIONS:
pI=8.59; Mw=35598
pI=8.49; Mw=35518
pI=8.22; Mw=35198

MAPPING (identification):
MICROSEQUENCING [1].



LYMPHOMA_HUMAN {Lymphoma}
Homo sapiens (Human)
Tissue: Lymphoma
LYMPHOMA_HUMAN
  map experimental info
  protein estimated location
 
LYMPHOMA_HUMAN

MAP LOCATIONS:
pI=9.24; Mw=35278
pI=8.99; Mw=35057
pI=8.48; Mw=34984
pI=8.28; Mw=34984
pI=8.74; Mw=34837

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [3].



PLATELET_HUMAN {Platelet}
Homo sapiens (Human)
Tissue: Platelet
PLATELET_HUMAN
  map experimental info
  protein estimated location
 
PLATELET_HUMAN

MAP LOCATIONS:
pI=8.73; Mw=35173
pI=8.10; Mw=35000
pI=8.42; Mw=35115
pI=7.83; Mw=34841
pI=7.61; Mw=34762

MAPPING (identification):
MATCHING WITH RBC AND LIVER MASTERS [4].



RBC_HUMAN {Red blood cells}
Homo sapiens (Human)
Tissue: Erythrocyte
RBC_HUMAN
  map experimental info
  protein estimated location
 
RBC_HUMAN

MAP LOCATIONS:
pI=9.65; Mw=34926
pI=9.41; Mw=34783
pI=9.15; Mw=34569
pI=8.96; Mw=34428
pI=8.70; Mw=34287
pI=8.45; Mw=34147

MAPPING (identification):
COMIGRATION [2].



U937_HUMAN {Macrophage Like Cell Line}
Homo sapiens (Human)
Tissue: Histiocytic lymphoma
U937_HUMAN
  map experimental info
  protein estimated location
 
U937_HUMAN

MAP LOCATIONS:
pI=8.95; Mw=35643
pI=8.69; Mw=35414
pI=7.91; Mw=35300
pI=8.24; Mw=35243

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [3].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://www.expasy.org/ch2d/license.html or send email to license@isb-sib.ch).
Cross-references
DOSAC-COBS-2DPAGEP04406; G3P_HUMAN.
HSC-2DPAGEP04406; HUMAN.
OGP-WWWP04406.
REPRODUCTION-2DPAGEIPI00219018; IPI00219018.
REPRODUCTION-2DPAGEP04406; G3P_HUMAN.
Siena-2DPAGEP04406; G3P2_HUMAN.
UniProtKB/Swiss-ProtP04406; G3P_HUMAN.
2D PAGE maps for unidentified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.
Arabidopsis thaliana
(Mouse-ear cress)
Arabidopsis thaliana
ARABIDOPSIS
Dictyostelium discoideum
(Slime mold)
Dictyostelium discoideum
DICTYSLUG
Escherichia coli
Escherichia coli
ECOLI
Escherichia coli DIGE (4.5-6.5)
ECOLI-DIGE4.5-6.5
Escherichia coli(4-5)
ECOLI4-5
Escherichia coli(4.5-5.5)
ECOLI4.5-5.5
Escherichia coli(5-6)
ECOLI5-6
Escherichia coli(5.5-6.7)
ECOLI5.5-6.7
Escherichia coli(6-11)
ECOLI6-11
Escherichia coli(6-9)
ECOLI6-9
Homo sapiens
(Human)
Colorectal epithelia cells
CEC_HUMAN
Cerebrospinal Fluid
CSF_HUMAN
Colorectal adenocarcinoma cell line (DL-1)
DLD1_HUMAN
Erythroleukemia Cell
ELC_HUMAN
HepG2 Secreted Proteins
HEPG2SP_HUMAN
HepG2
HEPG2_HUMAN
Promyelocytic leukemia cells
HL60_HUMAN
Kidney
KIDNEY_HUMAN
Lymphocytes
LYMPHOCYTE_HUMAN
Soluble nuclear proteins and matrix from liver tissue
NUCLEI_LIVER_HUMAN
SDS-PAGE of nucleolar proteins from Human HeLa cells
NUCLEOLI_HELA_1D_HUMAN
2D-PAGE of nucleolar proteins from Human HeLa cells
NUCLEOLI_HELA_2D_HUMAN
Plasma
PLASMA_HUMAN
Mus musculus
(Mouse)
Brown adipose tissue
BAT_MOUSE
Pancreatic islet cells
ISLETS_MOUSE
Liver
LIVER_MOUSE
Gastrocnemius muscle
MUSCLE_MOUSE
Soluble nuclear proteins and matrix from liver tissue
NUCLEI_LIVER_MOUSE
White adipose tissue
WAT_MOUSE
Saccharomyces cerevisiae
(Baker's yeast)
Saccharomyces cerevisiae
YEAST
Staphylococcus aureus
(strain N315)
Staphylococcus aureus
STAPHYLOCOCCUS



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 54.70
Entry nameG3P_HUMAN
Primary accession numberP04406
Secondary accession number(s) P00354
Name and origin of the protein
DescriptionGlyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) (GAPDH)
Gene nameName=GAPDH
Synonyms=GAPD
ORFNames=CDABP0047, OK/SW-cl.12
Keywords3D-structure; Cytoplasm; Direct protein sequencing; Glycolysis; Membrane; NAD; Oxidoreductase; Phosphoprotein; Polymorphism.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX01677; CAA25833.1; -; mRNA.
EMBLM17851; AAA86283.1; -; mRNA.
EMBLM33197; AAA52518.1; -; mRNA.
EMBLJ02642; AAA52496.1; -; mRNA.
EMBLJ04038; AAA53191.1; -; Genomic_DNA.
EMBLX53778; CAA37794.1; -; mRNA.
EMBLAF261085; AAF99678.1; -; mRNA.
EMBLAY007133; AAG01996.1; -; mRNA.
EMBLAB062273; BAB93466.1; -; mRNA.
EMBLAY340484; AAP88932.1; -; Genomic_DNA.
EMBLBT006893; AAP35539.1; -; mRNA.
EMBLBC001601; AAH01601.1; -; mRNA.
EMBLBC004109; AAH04109.1; -; mRNA.
EMBLBC009081; AAH09081.1; -; mRNA.
EMBLBC013310; AAH13310.1; -; mRNA.
EMBLBC023632; AAH23632.1; -; mRNA.
EMBLBC025925; AAH25925.1; -; mRNA.
EMBLBC026907; AAH26907.1; -; mRNA.
EMBLBC029618; AAH29618.1; -; mRNA.
EMBLBC083511; AAH83511.1; -; mRNA.
PIRA31988; DEHUG3.
RefSeqNP_002037.2; -.
UniGeneHs.479728; -.
UniGeneHs.544577; -.
UniGeneHs.592355; -.
UniGeneHs.648900; -.
PDB1U8F; X-ray; 1.75 A; O/P/Q/R=1-335.
PDB1ZNQ; X-ray; 2.50 A; O/P/Q/R=1-335.
PDB2FEH; Model; -; O/P/Q/R=1-335.
PDB3GPD; X-ray; 3.50 A; G/R=1-335.
PDBsum1U8F; -.
PDBsum1ZNQ; -.
PDBsum2FEH; -.
PDBsum3GPD; -.
IntActP04406; -.
SWISS-2DPAGEP04406; HUMAN.
Aarhus/Ghent-2DPAGE1206; NEPHGE.
Cornea-2DPAGEP04406; HUMAN.
DOSAC-COBS-2DPAGEP04406; HUMAN.
HSC-2DPAGEP04406; HUMAN.
OGPP04406; -.
REPRODUCTION-2DPAGEP04406; HUMAN.
Siena-2DPAGEP04406; -.
EnsemblENSG00000111640; Homo sapiens.
GeneID2597; -.
KEGGhsa:2597; -.
H-InvDBHIX0010367; -.
HGNCHGNC:4141; GAPDH.
HPACAB005197; -.
MIM138400; gene.
Orphanet372; Glyceraldehyde-3-phosphate dehydrogenase deficiency.
PharmGKBPA28554; -.
ReactomeREACT_1709; Metabolism of small molecules.
LinkHubP04406; -.
ArrayExpressP04406; -.
CleanExHS_GAPDH; -.
GermOnlineENSG00000111640; Homo sapiens.
GOGO:0005737; C:cytoplasm; NAS:UniProtKB.
GOGO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (p...; NAS:UniProtKB.
GOGO:0005515; F:protein binding; IPI:UniProtKB.
GOGO:0006096; P:glycolysis; NAS:UniProtKB.
InterProIPR000173; GAP_DH.
InterProIPR006424; GAPDH_I.
PANTHERPTHR10836; GAP_DH; 1.
PfamPF02800; Gp_dh_C; 1.
PfamPF00044; Gp_dh_N; 1.
PRINTSPR00078; G3PDHDRGNASE.
TIGRFAMsTIGR01534; GAPDH-I; 1.
PROSITEPS00071; GAPDH; 1.




Database constructed and maintained by Christine Hoogland, using the Make2D-DB II package (ver. 2.50.2) from the World-2DPAGE Constellation of the ExPASy web server