Sample Preparation and Post-separation Analysis

P00925

General information about the entry
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Entry name	ENO2_YEAST
Primary accession number	P00925
integrated into SWISS-2DPAGE on	February 1, 1995 (release 1)
2D Annotations were last modified on	October 1, 2001 (version 2)
General Annotations were last modified on	January 31, 2008 (version 9)
Name and origin of the protein
Description	Enolase 2 (EC 4.2.1.11) (2-phosphoglycerate dehydratase 2) (2-phospho-D-glycerate hydro-lyase 2).
Gene name	Name=ENO2 Synonyms=ENOB OrderedLocusNames=YHR174W
Annotated species	Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932]
Taxonomy	Eukaryota; Fungi; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
References
[1]   MAPPING ON GEL MEDLINE=96314060; PubMed=8740180; [NCBI, ExPASy, EBI, Israel, Japan] Sanchez J.-C., Golaz O., Frutiger S., Schaller D., Appel R.D., Bairoch A., Hughes G.J., Hochstrasser D.F. ''The yeast SWISS-2DPAGE database'' Electrophoresis 17:556-565(1996) [2]   MAPPING ON GEL MEDLINE=82271855; PubMed=7050667; [NCBI, ExPASy, EBI, Israel, Japan] Ludwig J.R., Foy J.J., Elliott S.G., McLaughlin C.S. ''Synthesis of specific identified, phosphorylated, heat shock, and heat stroke proteins through the cell cycle of Saccharomyces cerevisiae'' Mol. Cell. Biol. 2:117-126(1982) [3]   MAPPING ON GEL MEDLINE=89073918; PubMed=3332961; [NCBI, ExPASy, EBI, Israel, Japan] Bataille N., Peypouquet M.-F., Boucherie H. ''Identification of polypeptides of the carbon metabolism machinery on the two-dimensional protein map of Saccharomyces cerevisiae. Location of 23 additional polypeptides'' Yeast 3:11-21(1987)
Comments
SUBUNIT: HOMODIMER
2D PAGE maps for identified proteins
How to interpret a protein
YEAST {Saccharomyces cerevisiae} Saccharomyces cerevisiae (Baker's yeast)   map experimental info   protein estimated location		YEAST MAP LOCATIONS: SPOT 2D-000J73: pI=6.00; Mw=45000 SPOT 2D-000J7A: pI=5.87; Mw=45127 SPOT 2D-000J7C: pI=6.17; Mw=45127 SPOT 2D-000J87: pI=5.76; Mw=44222 SPOT 2D-000JZS: pI=6.15; Mw=11465 MAPPING (identification): AMINO ACID COMPOSITION ANALYSIS, MICROSEQUENCING [1] AND IDENTIFIED ON 2-D GELS BY LUDWIG ET AL [2] AND BATAILLE ET AL [3].
Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://www.expasy.org/ch2d/license.html or send email to license@isb-sib.ch).
Cross-references
COMPLUYEAST-2DPAGE	P00925.
UniProtKB/Swiss-Prot	P00925; ENO2_YEAST.
YEPD	6450.
2D PAGE maps for unidentified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.
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