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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P46527




SWISS-2DPAGE:  P46527


P46527


General information about the entry
View entry in simple text format
Entry nameCDN1B_HUMAN
Primary accession numberP46527
integrated into SWISS-2DPAGE on September 1, 1997 (release 6)
2D Annotations were last modified onDecember 1, 2000 (version 2)
General Annotations were last modified on January 31, 2008 (version 9)
Name and origin of the protein
DescriptionCyclin-dependent kinase inhibitor 1B (Cyclin-dependent kinase inhibitor p27) (p27Kip1).
Gene nameName=CDKN1B
Synonyms=KIP1
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
MEDLINE=97295311; PubMed=9150953; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Wirth P., Jaccoud S., Appel R.D., Sarto C., Wilkins M.R., Hochstrasser D.F.
''Simultaneous analysis of cyclin and oncogene expression using multiple monoclonal antibody immunoblots''
Electrophoresis 18:638-641(1997)
[2]   MAPPING ON GEL
Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A., Appel R.D., Hochstrasser D.F.
Submitted (JAN-1999) to the SWISS-2DPAGE database
2D PAGE maps for identified proteins
How to interpret a protein

DLD1_HUMAN {Colorectal adenocarcinoma cell line (DL-1)}
Homo sapiens (Human)
Tissue: Colon adenocarcinoma
DLD1_HUMAN
  map experimental info
  protein estimated location
 
DLD1_HUMAN

MAP LOCATIONS:
pI=6.31; Mw=23409
pI=6.46; Mw=23087
pI=6.86; Mw=22876

MAPPING (identification):
IMMUNOBLOTTING [2].



HEPG2_HUMAN {HepG2}
Homo sapiens (Human)
Tissue: Hepatoblastoma
HEPG2_HUMAN
  map experimental info
  protein estimated location
 
HEPG2_HUMAN

MAP LOCATIONS:
pI=6.56; Mw=25143
pI=6.96; Mw=25143
pI=6.72; Mw=24908

MAPPING (identification):
IMMUNOBLOTTING [1].



LIVER_HUMAN {Liver}
Homo sapiens (Human)
Tissue: Liver
LIVER_HUMAN
  map experimental info
  protein estimated location
 
LIVER_HUMAN

MAP LOCATIONS:
pI=7.31; Mw=24518

MAPPING (identification):
IMMUNOBLOTTING [1].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://www.expasy.org/ch2d/license.html or send email to license@isb-sib.ch).
Cross-references
UniProtKB/Swiss-ProtP46527; CDN1B_HUMAN.
2D PAGE maps for unidentified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.
Arabidopsis thaliana
(Mouse-ear cress)
Arabidopsis thaliana
ARABIDOPSIS
Dictyostelium discoideum
(Slime mold)
Dictyostelium discoideum
DICTYSLUG
Escherichia coli
Escherichia coli
ECOLI
Escherichia coli DIGE (4.5-6.5)
ECOLI-DIGE4.5-6.5
Escherichia coli(4-5)
ECOLI4-5
Escherichia coli(4.5-5.5)
ECOLI4.5-5.5
Escherichia coli(5-6)
ECOLI5-6
Escherichia coli(5.5-6.7)
ECOLI5.5-6.7
Escherichia coli(6-11)
ECOLI6-11
Escherichia coli(6-9)
ECOLI6-9
Homo sapiens
(Human)
Colorectal epithelia cells
CEC_HUMAN
Cerebrospinal Fluid
CSF_HUMAN
Erythroleukemia Cell
ELC_HUMAN
HepG2 Secreted Proteins
HEPG2SP_HUMAN
Promyelocytic leukemia cells
HL60_HUMAN
Kidney
KIDNEY_HUMAN
Lymphocytes
LYMPHOCYTE_HUMAN
Lymphoma
LYMPHOMA_HUMAN
Soluble nuclear proteins and matrix from liver tissue
NUCLEI_LIVER_HUMAN
SDS-PAGE of nucleolar proteins from Human HeLa cells
NUCLEOLI_HELA_1D_HUMAN
2D-PAGE of nucleolar proteins from Human HeLa cells
NUCLEOLI_HELA_2D_HUMAN
Plasma
PLASMA_HUMAN
Platelet
PLATELET_HUMAN
Red blood cells
RBC_HUMAN
Macrophage Like Cell Line
U937_HUMAN
Mus musculus
(Mouse)
Brown adipose tissue
BAT_MOUSE
Pancreatic islet cells
ISLETS_MOUSE
Liver
LIVER_MOUSE
Gastrocnemius muscle
MUSCLE_MOUSE
Soluble nuclear proteins and matrix from liver tissue
NUCLEI_LIVER_MOUSE
White adipose tissue
WAT_MOUSE
Saccharomyces cerevisiae
(Baker's yeast)
Saccharomyces cerevisiae
YEAST
Staphylococcus aureus
(strain N315)
Staphylococcus aureus
STAPHYLOCOCCUS



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 54.70
Entry nameCDN1B_HUMAN
Primary accession numberP46527
Secondary accession number(s) Q16307 Q9BUS6
Name and origin of the protein
DescriptionCyclin-dependent kinase inhibitor 1B (Cyclin-dependent kinase inhibitor p27) (p27Kip1)
Gene nameName=CDKN1B
Synonyms=KIP1
Keywords3D-structure; Cell cycle; Cytoplasm; Direct protein sequencing; Nucleus; Phosphoprotein; Polymorphism; Protein kinase inhibitor; Proto-oncogene.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLU10906; AAA20240.1; -; mRNA.
EMBLS76988; AAD14244.1; -; Genomic_DNA.
EMBLS76986; AAD14244.1; JOINED; Genomic_DNA.
EMBLAF480891; AAL78041.1; -; Genomic_DNA.
EMBLBC001971; AAH01971.1; -; mRNA.
RefSeqNP_004055.1; -.
UniGeneHs.238990; -.
PDB1H27; X-ray; 2.20 A; E=25-35.
PDB1JSU; X-ray; 2.30 A; C=23-106.
PDB2AST; X-ray; 2.30 A; D=181-190.
PDBsum1H27; -.
PDBsum1JSU; -.
PDBsum2AST; -.
DisProtDP00018; -.
IntActP46527; -.
SWISS-2DPAGEP46527; HUMAN.
EnsemblENSG00000111276; Homo sapiens.
GeneID1027; -.
KEGGhsa:1027; -.
H-InvDBHIX0010441; -.
HGNCHGNC:1785; CDKN1B.
HPACAB003691; -.
MIM600778; gene.
MIM610755; phenotype.
PharmGKBPA105; -.
LinkHubP46527; -.
ArrayExpressP46527; -.
CleanExHS_CDKN1B; -.
GermOnlineENSG00000111276; Homo sapiens.
GOGO:0005737; C:cytoplasm; IDA:HGNC.
GOGO:0005634; C:nucleus; IDA:HGNC.
GOGO:0004861; F:cyclin-dependent protein kinase inhibitor a...; TAS:ProtInc.
GOGO:0005515; F:protein binding; IPI:IntAct.
GOGO:0005072; F:transforming growth factor beta receptor; c...; TAS:ProtInc.
GOGO:0048102; P:autophagic cell death; IDA:UniProtKB.
GOGO:0007050; P:cell cycle arrest; IMP:HGNC.
GOGO:0000082; P:G1/S transition of mitotic cell cycle; IDA:UniProtKB.
GOGO:0006917; P:induction of apoptosis; IDA:UniProtKB.
GOGO:0030308; P:negative regulation of cell growth; IDA:UniProtKB.
GOGO:0008285; P:negative regulation of cell proliferation; TAS:ProtInc.
GOGO:0042326; P:negative regulation of phosphorylation; IDA:UniProtKB.
GOGO:0000079; P:regulation of cyclin-dependent protein kina...; TAS:ProtInc.
InterProIPR003175; CDI.
InterProIPR015456; p27_Kip1.
PANTHERPTHR10265; CDI; 1.
PANTHERPTHR10265:SF4; p27_Kip1; 1.
PfamPF02234; CDI; 1.




Database constructed and maintained by Christine Hoogland, using the Make2D-DB II package (ver. 2.50.2) from the World-2DPAGE Constellation of the ExPASy web server

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