Sample Preparation and Post-separation Analysis

P02765

General information about the entry
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Entry name	FETUA_HUMAN
Primary accession number	P02765
integrated into SWISS-2DPAGE on	August 1, 1993 (release 0)
2D Annotations were last modified on	May 15, 2003 (version 1)
General Annotations were last modified on	January 31, 2008 (version 14)
Name and origin of the protein
Description	Alpha-2-HS-glycoprotein (Fetuin-A) (Alpha-2-Z-globulin) (Ba-alpha-2-glycoprotein) [Contains: Alpha-2-HS-glycoprotein chain A; Alpha-2-HS-glycoprotein chain B].
Gene name	Name=AHSG Synonyms=FETUA ORFNames=PRO2743
Annotated species	Homo sapiens (Human) [TaxID: 9606]
Taxonomy	Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL MEDLINE=78094420; PubMed=271964; [NCBI, ExPASy, EBI, Israel, Japan] Anderson N.L., Anderson N.G. ''High resolution two-dimensional electrophoresis of human plasma proteins'' Proc. Natl. Acad. Sci. U.S.A. 74:5421-5425(1977) [2]   MAPPING ON GEL MEDLINE=96007934; PubMed=7498157; [NCBI, ExPASy, EBI, Israel, Japan] Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch A., Hochstrasser D.F. ''Inside SWISS-2DPAGE database'' Electrophoresis 16:1131-1151(1995)
Comments
SUBUNIT: A AND B CHAIN
2D PAGE maps for identified proteins
How to interpret a protein
CSF_HUMAN {Cerebrospinal Fluid} Homo sapiens (Human) Tissue: Cerebrospinal fluid   map experimental info   protein estimated location		CSF_HUMAN MAP LOCATIONS: SPOT 2D-000BTG: pI=4.43; Mw=60957 SPOT 2D-000BU5: pI=4.47; Mw=59576 SPOT 2D-000BU7: pI=4.42; Mw=59576 SPOT 2D-000BUA: pI=4.51; Mw=58672 SPOT 2D-000BUU: pI=4.55; Mw=58077 SPOT 2D-000BV6: pI=4.46; Mw=57930 SPOT 2D-000BVY: pI=4.50; Mw=57051 MAPPING (identification): MATCHING WITH THE PLASMA MASTER GEL [2].
HEPG2SP_HUMAN {HepG2 Secreted Proteins} Homo sapiens (Human) Tissue: Hepatoblastoma   map experimental info   protein estimated location		HEPG2SP_HUMAN MAP LOCATIONS: SPOT 2D-0008W0: pI=4.16; Mw=58201 SPOT 2D-0008WF: pI=4.21; Mw=57256 SPOT 2D-0008WM: pI=4.26; Mw=56882 SPOT 2D-0008WY: pI=4.33; Mw=55775 SPOT 2D-0008XO: pI=4.39; Mw=55050 SPOT 2D-0008Y2: pI=4.43; Mw=54690 SPOT 2D-0008YA: pI=4.28; Mw=53979 SPOT 2D-0008YC: pI=4.48; Mw=52583 SPOT 2D-0008YR: pI=4.33; Mw=53102 SPOT 2D-0008YX: pI=4.43; Mw=52240 SPOT 2D-0008Z0: pI=4.39; Mw=52411 MAPPING (identification): MATCHING WITH THE PLASMA MASTER GEL [2].
PLASMA_HUMAN {Plasma} Homo sapiens (Human) Tissue: Plasma   map experimental info   protein estimated location		PLASMA_HUMAN MAP LOCATIONS: SPOT 2D-0004QJ: pI=4.56; Mw=57706 SPOT 2D-0004R8: pI=4.58; Mw=56773 SPOT 2D-0004RK: pI=4.56; Mw=56466 SPOT 2D-0004RS: pI=4.61; Mw=56313 SPOT 2D-0004S9: pI=4.58; Mw=55553 SPOT 2D-0004SB: pI=4.61; Mw=55403 SPOT 2D-0004SY: pI=4.64; Mw=54804 SPOT 2D-0004T4: pI=4.68; Mw=54359 SPOT 2D-0004TI: pI=4.60; Mw=54212 SPOT 2D-0004TM: pI=4.64; Mw=54065 SPOT 2D-0004TY: pI=4.68; Mw=53336 SPOT 2D-0004U3: pI=4.71; Mw=53191 SPOT 2D-0004UA: pI=4.64; Mw=52903 SPOT 2D-0004V2: pI=4.71; Mw=52474 SPOT 2D-0004VB: pI=4.67; Mw=52474 NORMAL LEVEL: 400-850 MG/L. PATHOLOGICAL LEVEL: DECREASED DURING TUMORS AND INFLAMMATORY PROCESSES. MAPPING (identification): IMMUNOBLOTTING [1].
Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://www.expasy.org/ch2d/license.html or send email to license@isb-sib.ch).
Cross-references
DOSAC-COBS-2DPAGE	P02765; FETUA_HUMAN.
Siena-2DPAGE	P02765; A2HS_HUMAN.
UniProtKB/Swiss-Prot	P02765; FETUA_HUMAN.
2D PAGE maps for unidentified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.
Arabidopsis thaliana (Mouse-ear cress)	Arabidopsis thaliana
Dictyostelium discoideum (Slime mold)	Dictyostelium discoideum
Escherichia coli	Escherichia coli Escherichia coli DIGE (4.5-6.5) Escherichia coli(4-5) Escherichia coli(4.5-5.5) Escherichia coli(5-6) Escherichia coli(5.5-6.7) Escherichia coli(6-11) Escherichia coli(6-9)
Homo sapiens (Human)	Colorectal epithelia cells Colorectal adenocarcinoma cell line (DL-1) Erythroleukemia Cell HepG2 Promyelocytic leukemia cells Kidney Liver Lymphocytes Lymphoma Soluble nuclear proteins and matrix from liver tissue SDS-PAGE of nucleolar proteins from Human HeLa cells 2D-PAGE of nucleolar proteins from Human HeLa cells Platelet Red blood cells Macrophage Like Cell Line
Mus musculus (Mouse)	Brown adipose tissue Pancreatic islet cells Liver Gastrocnemius muscle Soluble nuclear proteins and matrix from liver tissue White adipose tissue
Saccharomyces cerevisiae (Baker's yeast)	Saccharomyces cerevisiae
Staphylococcus aureus (strain N315)	Staphylococcus aureus