PROSITE entry PS51318

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General information about the entry

PURL [info]	https://purl.expasy.org/prosite/signature/PS51318
Entry name [info]	TAT
Accession [info]	PS51318
Entry type [info]	MATRIX
Date [info]	01-MAY-2007 CREATED; 26-FEB-2020 DATA UPDATE; 29-MAY-2024 INFO UPDATE.
PROSITE Doc. [info]	PDOC51318
Associated ProRule [info]	PRU00648

Name and characterization of the entry

Description [info]

Twin arginine translocation (Tat) signal profile.

Matrix / Profile [info]

/GENERAL_SPEC: ALPHABET='ACDEFGHIKLMNPQRSTVWY'; LENGTH=40;
/DISJOINT: DEFINITION=PROTECT; N1=5; N2=36;
/NORMALIZATION: MODE=1; FUNCTION=LINEAR; R1=4.1003532; R2=0.0044078; TEXT='NScore';
/NORMALIZATION: MODE=-1; FUNCTION=LINEAR; R1=2985.9531250; R2=1.0537143; PRIORITY=1; TEXT='Heuristic 5.0%';
/CUT_OFF: LEVEL=0; SCORE=794; H_SCORE=3823; N_SCORE=7.6; MODE=1; TEXT='!';
/CUT_OFF: LEVEL=-1; SCORE=545; H_SCORE=3560; N_SCORE=6.5; MODE=1; TEXT='?';
/DEFAULT: M0=-28; D=-200; I=-200; B0=*; B1=*; E1=-300; MI=-205; MD=-205; IM=-205; DM=-205;

...

                A    C    D    E    F    G    H    I    K    L    M    N    P    Q    R    S    T    V    W    Y
/I:         I=-50; B0=0; BI=-205; BD=-205;
/M: SY='M';  M= -51, -59, -74, -63, -41, -66, -44, -25, -56, -18, 142, -60, -61, -48, -54, -61, -50, -30, -64, -40; D=-50;
/I:         I=-50;
/M: SY='S';  M=  -4, -22,  -4,  -8, -36, -17,  -6, -26,  16, -27, -13,  22,  12,  -4,  -2,  40,  37, -32, -47, -37; D=-5;
/I:         I=-4; MD=-17;
/M: SY='D';  M= -15, -38,  28,   8, -27, -24,  12, -15,  18, -25,   7,  21, -11,  19,  13,   6,   6, -29, -41, -30; D=-1;
/I:         I=-4; MD=-17;
/M: SY='H';  M= -24, -60,  10,   4, -19, -29,  19, -16,  14, -18, -15,   5,  18,  13,   7,  13,  13, -24, -33, -30; D=-1;
/I:         I=-4; MD=-17;
/M: SY='P';  M= -18, -36,  14,   0, -21, -26,   9, -21,  12, -25, -15,  16,  24,   4,  -3,   5,   7, -32, -38, -42; D=-1;
/I:         I=-4; MD=-17;
/M: SY='P';  M= -21, -42,   2,  -1, -22, -19,  -4, -22,   4, -27, -31,  12,  22,   6,  -1,   6,   8, -30, -37, -35; D=-1;
/I:         I=-4; MD=-17;
/M: SY='P';  M= -13, -25,   2, -13, -15, -27,  -3, -26,  -5, -25, -19,   5,  21,  -8,  -4,  12,   0, -38, -41, -38; D=-1;
/I:         I=-4; MD=-17;
/M: SY='P';  M=  -9, -39,  -4, -13, -31, -17,  -4, -43, -12, -30, -35,   2,  18,  -5,  -2,   6,  -2, -36, -23, -51; D=-1;
/I:         I=-4; MD=-17;
/M: SY='P';  M= -12, -43, -15, -16, -44,  -9, -13, -36, -23, -27, -26,  -9,  10,  -1,  -5,   1, -16, -32, -41, -48; D=-1;
/I:         I=-4; MD=-17;
/M: SY='P';  M= -11, -42, -20, -19, -65, -16, -19, -41, -30, -26, -22, -16,  -2, -19, -19, -11, -10, -39, -51, -67; D=-1;
/I:         I=-4; MI=0; MD=-17; IM=0; DM=-17;
/M: SY='G';  M= -22, -67, -21, -44, -53,  10, -37, -76, -25, -65, -33, -14, -16, -20, -12, -17, -20, -65, -47, -42; D=-1;
/I:         I=-4; DM=-17;
/M: SY='M';  M= -38, -54, -63, -60,   8, -60, -44,  29, -59,  36,  38, -41,  10, -28, -52, -38,  -5,  21, -25, -37; D=-1;
/I:         I=-17; DM=-17;
/M: SY='S';  M= -28, -57,  13, -33, -65,   0, -25, -62, -32, -61, -55,  33, -28,  -9, -36,  73,  48, -55, -75, -58;
/M: SY='R';  M=-115,-131,-111, -99,-123,-125,-100,-130, -40,-123,-111,-104,-123, -87, 124,-109,-113,-124,-123,-110;
/M: SY='R';  M=-115,-131,-111, -99,-123,-113,-100,-130, -64,-123,-111,-104,-123, -87, 124,-109,-113,-124, -78,-110;
/M: SY='D';  M=  -2, -55,  43,  -1, -62,   8,  18, -64,  -4, -61, -50,  14, -57,  40,  35,  24,  14, -53, -73, -54;
/M: SY='F';  M= -22, -88,-133,-127, 108,-116,-102, -27,-124,  30,   9,-125,-108,-110,-122,-106, -95,   4,   2, -67;
/M: SY='L';  M=-101, -66,-132,-125, -18,-120, -95,  26,-124,  84,  48,-102,-129,-118,-122,-113, -84,   1,-119,-100;
/M: SY='K';  M=   5, -58, -48, -25, -52,  12, -22, -42,  63, -33, -21, -22, -57,  39,  33,  -7,   0, -35, -52, -39;
/M: SY='G';  M=  29, -43, -44, -53,  -7,  41, -33, -29, -24,   4,   2, -17, -60,  -8,  -4,   1,  28, -16,  -6, -15;
/M: SY='A';  M=  46,  18, -58, -56, -34,  20, -58, -19, -55,   6,  15, -46, -53, -52, -58,  34,  29,  -4, -68, -56;
/M: SY='A';  M=  58, -25, -57, -56, -51,  48, -59, -39, -54,  -1, -15, -47, -48, -51, -60,  10,  13, -14, -67, -59;
/M: SY='A';  M=  56, -38, -59, -57, -23,  20, -54,  -1, -35,  12,  10, -51, -61, -40, -41,  -5,   3,  12, -23, -41;
/M: SY='A';  M=  46, -38, -60, -58, -21,  34, -60, -17, -54,  20,   2, -52, -56, -55, -61,   2,  23,  12, -66, -48;
/M: SY='A';  M=  52, -38, -56, -57, -41,  46, -60, -16, -56,  -6, -18, -49, -38, -54, -60,  10,  21,   3, -56, -58;
/M: SY='A';  M=  55, -31, -61, -59, -34,  36, -61, -11, -58,  18, -11, -53, -37, -55, -59, -15,  -3,  17, -66, -56;
/M: SY='A';  M=  53, -35, -59, -57, -27,  34, -59, -30, -56,  11,   1, -48, -53, -51, -60,  16,  16,   5, -50, -42;
/M: SY='A';  M=  47, -26, -62, -58, -23,  20, -55,  -6, -57,  26,  15, -52, -21, -49, -60,  -2,   8,  15, -43, -45;
/M: SY='A';  M=  53, -26, -60, -54, -22,  25, -60, -23, -56,   9,   9, -47,  -7, -49, -58,   7,   7,  14, -21, -42;
/M: SY='L';  M=  36, -28, -61, -57,  11,  -4, -60,   8, -60,  38,  23, -55, -43, -52, -62,  -9,  14,  14, -26, -38;
/I:         I=-23; MD=-24;
/M: SY='G';  M=  40, -53, -49, -54, -38,  47, -46, -32, -57, -13, -21, -40,  22, -39, -55,  18,  -4, -20, -35, -46; D=-23;
/I:         I=-23; MD=-24;
/M: SY='G';  M=  25, -21, -48, -54, -27,  34, -45, -40, -52, -12, -19, -36,  13, -29, -29,  26,   8, -27, -32, -37; D=-23;
/I:         I=-23; MD=-24;
/M: SY='A';  M=  22,   6, -66, -62, -25,   7, -24, -33, -54,   8,  -8, -32, -34, -40, -54,  11,  -3, -11,  14, -36; D=-23;
/I:         I=-23; MI=0; MD=-24; IM=0; DM=-24;
/M: SY='M';  M=   8, -27, -58, -63,  13,   3, -53, -26, -71,   0,  17, -58, -40, -52, -68,  -5,  -7, -23,  -4, -58; D=-23;
/I:         I=-23; DM=-24;
/M: SY='A';  M=  27, -62, -47, -44, -19,  -9, -43, -19, -39,  19,   9, -10, -43,  -8,  -6,  -1,   2,  -6, -18, -44; D=-23;
/I:         I=-23; DM=-24;
/M: SY='P';  M= -41, -75, -38, -28, -71,   1, -31, -66, -28, -67, -58, -34, 100, -23,  -6,  18,   0, -59, -73, -66; D=-23;
/I:         I=-23; MI=0; MD=-24; IM=0; DM=-24;
/M: SY='A';  M=  20, -55, -29,  -8, -43, -11, -30, -13, -11, -15, -21, -26, -20,   5,  11,   3,   7,   1, -38, -36; D=-23;
/I:         I=-23; DM=-24;
/M: SY='A';  M=  94, -93,-110, -85,-120, -60,-116, -88, -97, -54, -66,-109, -58, -30, -51,   7, -34,   6,-125,-117;
/M: SY='W';  M=   7, -49, -10,   5,  10, -29,  27, -41, -11,  -1,   0,  -8,   3,  36,  16,   9,   1, -27,  45, -15;
/M: SY='A';  M= 105,-109,-100, -93,-123, -72,-117,-109, -84,-110,-109,-109, -96, -81, -83, -51, -66, -94,-123,-109;
/I:         E1=0; IE=-205; DE=-205;

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Post-processing [info]

COMPETES_HIT_WITH

PS51257

Numerical results [info]

Numerical results for UniProtKB/Swiss-Prot release 2025_02 which contains 573'230 sequence entries.

Total number of hits	614 in 614 different sequences
Number of true positive hits	570 in 570 different sequences
Number of 'unknown' hits	26 in 26 different sequences
Number of false positive hits	18 in 18 different sequences
Number of false negative sequences	49
Number of 'partial' sequences	0

Precision (true positives / (true positives + false positives))	96.94 %
Recall (true positives / (true positives + false negatives))	92.08 %

Comments [info]

Taxonomic range [info]	Archaea, Eukaryotes, Prokaryotes (Bacteria)
Maximum number of repetitions [info]	1
Matrix type [info]	protein_domain
Scaling database [info]	reversed
Author [info]	N_Hulo
Feature key [info]	SIGNAL
Feature description [info]	Tat-type signal (Potential)
Version [info]	6

Cross-references [info]

UniProtKB/Swiss-Prot True positive sequences	570 sequences AAUA_ALCFA (P84887), AEEP_BACTN (Q8A861), AGAR_STRCO (P07883), » more Retrieve an alignment of UniProtKB/Swiss-Prot true positive hits: [Clustal format, color, condensed view] [Clustal format, color] [Clustal format, plain text] [Fasta format] Retrieve the sequence logo from the alignment
UniProtKB/Swiss-Prot False negative sequences	49 sequences AMIA_SALTY (P33772), BLAC_MYCBO (P0A5I7), BLAC_MYCTA (A5U493), » more
UniProtKB/Swiss-Prot 'Unknown' sequences	26 sequences A85B_MYCSC (Q50397), BASB_HALS3 (B0R6I5), CDGT1_NIACI (P30920), » more
UniProtKB/Swiss-Prot False positive sequences	18 sequences A85A_MYCAV (O52956), A85A_MYCBO (P0C2T1), A85A_MYCBP (A1KQD8), » more
PDB [Detailed view]	111 PDB 1DMR; 1E18; 1E5V; 1E60; 1E61; 1H2A; 1H5N; 1H6A; 1H6B; 1H6C; 1H6D; 1HFE; » more