PROSITE entry PS50222

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General information about the entry

PURL [info]	https://purl.expasy.org/prosite/signature/PS50222
Entry name [info]	EF_HAND_2
Accession [info]	PS50222
Entry type [info]	MATRIX
Date [info]	01-JUN-2005 CREATED; 01-OCT-2013 DATA UPDATE; 29-MAY-2024 INFO UPDATE.
PROSITE Doc. [info]	PDOC00018
Associated ProRule [info]	PRU00448

Name and characterization of the entry

Description [info]

EF-hand calcium-binding domain profile.

Matrix / Profile [info]

/GENERAL_SPEC: ALPHABET='ACDEFGHIKLMNPQRSTVWY'; LENGTH=34;
/DISJOINT: DEFINITION=PROTECT; N1=1; N2=34;
/NORMALIZATION: MODE=1; FUNCTION=LINEAR; R1=4.9731784; R2=0.0278954; TEXT='NScore';
/CUT_OFF: LEVEL=0; SCORE=116; N_SCORE=8.2; MODE=1; TEXT='R';
/CUT_OFF: LEVEL=-1; SCORE=1; N_SCORE=5.0; MODE=1; TEXT='R?';
/DEFAULT: B0=-100; B1=-150; E1=-150; MM=*; II=*;

...

                A    C    D    E    F    G    H    I    K    L    M    N    P    Q    R    S    T    V    W    Y
/I:         B1=-85; E0=*; BD=-85;
/M: SY='E';  M=  -8, -23,  -2,   3, -15, -17,  -9, -13,  -8, -10,  -7,  -5, -10,  -2,  -6,  -4,  -5, -11, -21,  -9; M0=-3;
/I:         MM=0; MI=-137; MD=-56; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='E';  M=  -5, -22,   1,   4, -20, -14,  -6, -16,  -2, -16, -10,   0,  -6,   1,  -5,   2,  -1, -14, -27, -13; M0=-3;
/I:         MM=0; MI=-137; MD=-56; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='E';  M=  -8, -26,   7,  15, -19, -16, -19,   7,   9,   0,   4,  -8, -11,   0,  -1,  -6,  -7,  -2, -21,  -9; M0=-3;
/I:         MM=0; MI=-137; MD=-56; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='E';  M=   7, -71,  12,  17, -17, -13,  -5, -17,   9, -12, -10,  -2,  -7,   9,  -3,   1,  -4, -19, -73, -66; M0=-7;
/I:         MM=0; MI=-136; MD=-86; IM=-9; II=-11; DM=-22; DD=-3;
/M: SY='E';  M= -13, -16,  10,  11, -10, -13,   1,  -2,   6, -14,  -1,  -8, -24,   5,   6, -14,  -8,   0, -21,  -4; M0=-5;
/I:         MM=0; MI=-136; MD=-147; IM=-9; II=-11; DM=-1; DD=-40;
/M: SY='L';  M=   3,   6, -40, -42,  12, -30, -27,  13, -27,  17,   3, -29, -26, -28, -30, -12, -15,  11,  14,   2; M0=-12;
/I:         MM=0; MI=-137; MD=-147; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='K';  M=  -4, -27,   9,  13, -14, -17,  -7, -12,  17, -11,   2,  -3, -30,  11,  14, -15, -11, -15,  -6, -16; M0=-6;
/I:         MM=0; MI=-137; MD=-147; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='E';  M=   3, -16,   4,  16,  -3, -22,   0, -18,  15, -24, -29,   6, -67,   9,   5,  -3,  -4, -16,  -4,  -5; M0=-8;
/I:         MM=0; MI=-137; MD=-147; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='M';  M=  14,   3, -32, -28,   3, -21, -20,  17, -20,   5,  20, -39, -41, -18,  -9, -14,  -3,   9,  -5,  -8; M0=-9;
/I:         MM=0; MI=-137; MD=-147; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='F';  M= -31, -16, -83, -43,  39, -75, -27,   7, -73,  -1,  11, -72, -76, -69, -71, -66, -59,  -9,  20,   2; M0=-39;
/I:         MM=0; MI=-86; MD=-147; IM=-2; II=-32; DM=-7; DD=-14;
/M: SY='K';  M=   1, -17,  12,   5, -29, -35,   4, -20,  17, -17,  -1,   6, -41,  11,  16,  -1, -18, -25, -73, -32; M0=-10;
/I:         MM=0; MI=-137; MD=-147; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='E';  M=   2, -10,   1,  10, -10, -34,   6,  -4,   8,   7,  10,  -6, -67,   2,   0,  -3,  -4,   2, -72, -13; M0=-6;
/I:         MM=0; MI=-137; MD=-147; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='F';  M=  -2, -10, -42, -44,  27, -36,  -1,   7, -25,  10,  13, -25, -75, -23, -30, -15, -27,   7,  -5,  19; M0=-16;
/I:         MM=0; MI=-137; MD=-147; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='D';  M= -35,-100,  41, -30,-103, -29, -86,-103, -77, -49, -95, -22, -32, -25, -87, -18, -41, -35,-103, -92; M0=-47;
/I:         MM=0; MI=-137; MD=-147; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='K';  M=  -1, -34, -15,  -1,  -6, -19, -18,  -4,  19,  -5,  -9, -10,  -7,   4,  11,  -5,   9,   3, -22, -33; M0=-4;
/I:         MM=0; MI=-137; MD=-147; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='D';  M= -23, -23,  33, -10, -74, -21, -29, -72,  -6, -71, -62,  25, -39, -21, -21, -17, -15, -67, -23, -35; M0=-28;
/I:         MM=0; MI=-137; MD=-147; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='G';  M= -11,  -7,  -2,  -9, -37,  24,   8, -72,  14, -35,  -9,   7, -67,   2,   6,  -4, -22, -28, -73, -17; M0=-15;
/I:         MM=0; MI=-137; MD=-147; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='D';  M= -25, -26,  31, -13, -74, -25, -26, -72,   0, -34, -27,  19, -67, -15, -30,  12,   0, -42, -73, -66; M0=-24;
/I:         MM=0; MI=-137; MD=-147; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='G';  M= -34, -25, -19, -30, -74,  36, -15, -31, -22, -33, -14,  -4, -39, -15, -18, -25, -30, -67, -11, -66; M0=-26;
/I:         MM=0; MI=-137; MD=-61; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='Y';  M= -10,  -4, -25,   0,  13, -25,   3,  -8,  14,  -8,  -6,  -8, -67,   7,   6,  -3,   8,   1, -22,  14; M0=-6;
/I:         MM=0; MI=-56; MD=-147; IM=-4; II=-22; DM=-11; DD=-9;
/M: SY='I';  M= -30, -75,-104, -99, -18,-100, -91,  32, -42,  12,   2, -97, -97, -92, -95, -92, -78,  17, -85, -32; M0=-54;
/I:         MM=0; MI=-137; MD=-147; IM=-9; II=-11; DM=-2; DD=-30;
/M: SY='D';  M= -43, -10,  22,   0, -38,  -8,  -2, -32, -26, -30, -30,   5,  -9, -11, -20,  20,  17, -65, -60, -62; M0=-17;
/I:         MM=0; MI=-137; MD=-147; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='F';  M=   1, -16, -31,  -6,  22, -10, -15,  -1,   5,   0,  -7, -11,  -1,  -9,   7, -10,  -8,   1,   8,  10; M0=-4;
/I:         MM=0; MI=-137; MD=-147; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='E';  M=  -1, -25,  16,  20, -22, -10,  -4, -28,   6, -14, -14,   7, -14,   9, -12,  -3,  -6, -22, -60, -12; M0=-7;
/I:         MM=0; MI=-137; MD=-147; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='E';  M= -26, -71,  13,  36, -38, -66, -14, -72, -25, -46, -31, -14, -67,  -4, -54, -26, -32, -35, -73, -21; M0=-31;
/I:         MM=0; MI=-137; MD=-147; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='F';  M= -29, -23, -83, -77,  31, -75, -29,   2, -73,  18,   9, -71, -75, -69, -71, -66, -25,   2,  12,  11; M0=-38;
/I:         MM=0; MI=-137; MD=-147; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='K';  M=  -2,   7, -16,   0, -19,  -3,   2,   3,  13,   2,  -1,  -6, -41,   6,  11,  -4,  -4,   4, -21, -15; M0=-3;
/I:         MM=0; MI=-137; MD=-147; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='Q';  M=   3, -13,  10,   9, -19,  -7,  -7, -18,   4, -11,   2,   3, -20,  14,   3,  -1,   0,  -7, -22,   2; M0=-3;
/I:         MM=0; MI=-137; MD=-147; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='M';  M=  13,   9, -33, -43,  14,  -2,  -5,   9, -17,   7,  19, -38, -38, -26, -30, -13, -13,   7,   7,   2; M0=-10;
/I:         MM=0; MI=-137; MD=-147; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='M';  M=  -4,   4, -29, -76,  13, -35, -13,   9, -44,  18,  26, -30, -18, -37, -32, -44, -20,   9, -12,  -2; M0=-17;
/I:         MM=0; MI=-137; MD=-147; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='K';  M=  -2, -25,  -9,   5, -17,  -7,   9,  -7,  14, -11,  -7,  -3, -17,   7,  12,   2,   6,  -5, -73,  -8; M0=-4;
/I:         MM=-88; MI=0; MD=-147; IM=0; II=-114; DM=-7; DD=-14;
/M: SY='L';  M=  -3,  -1, -13,  -8,   9, -13,  -5,   6,  -3,  14,  12,  -6, -40,  -4, -12,  -3,   0,   4, -12,  -1; M0=-4;
/I:         MM=0; MI=-137; MD=-147; IM=-9; II=-11; DM=-7; DD=-14;
/M: SY='G';  M=  -2,   5,  -3,  -5, -11,  20,  -2, -11,   1,   0,   5,  -2,  -7,  -2, -18,  -6,  -2,  -4, -19,   0; M0=-3;
/I:         MM=-78; MI=0; MD=-147; IM=0; II=-114; DM=-7; DD=-14;
/M: SY='H';  M=  -4, -10,   7,   8,  -5,  -3,  12, -13,   9,  -3,  -6,   8,   5,  -1,  -2,   0,  -7, -19, -11,  -8; M0=-2;
/I:         B0=*; E1=-10;

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Numerical results [info]

Numerical results for UniProtKB/Swiss-Prot release 2025_02 which contains 573'230 sequence entries.

Total number of hits	4'789 in 1'744 different sequences
Number of true positive hits	4'770 in 1'725 different sequences
Number of 'unknown' hits	0
Number of false positive hits	19 in 19 different sequences
Number of false negative sequences	120
Number of 'partial' sequences	8

Precision (true positives / (true positives + false positives))	99.60 %
Recall (true positives / (true positives + false negatives))	97.55 %

Comments [info]

Taxonomic range [info]	Bacteriophages, Eukaryotes, Prokaryotes (Bacteria), Eukaryotic viruses
Maximum number of repetitions [info]	17
Matrix type [info]	protein_domain
Scaling database [info]	window20
Author [info]	N_Hulo
Feature key [info]	DOMAIN
Feature description [info]	EF-hand
Version [info]	4

Cross-references [info]

UniProtKB/Swiss-Prot True positive sequences	1725 sequences 2AB2A_ARATH (Q9XGR4), 2AB2B_ARATH (Q5QIT3), 2AB2C_ARATH (Q8VZQ4), » more Retrieve an alignment of UniProtKB/Swiss-Prot true positive hits: [Clustal format, color, condensed view] [Clustal format, color] [Clustal format, plain text] [Fasta format] Retrieve the sequence logo from the alignment
UniProtKB/Swiss-Prot False negative sequences	120 sequences AIN1_SCHPO (O13728), CAMK1_ARATH (O80673), CAMK2_ARATH (Q9LJL9), » more
UniProtKB/Swiss-Prot 'Partial' sequences	8 sequences CALM_TETTH (Q05055), MYL_PENMO (P86703), PRVA_RANTE (P84535), » more
UniProtKB/Swiss-Prot False positive sequences	19 sequences ARMET_DROAN (B3M2I7), ARMET_DROPE (B4GFM7), ARMET_DROPS (Q295V5), » more
PDB [Detailed view]	1462 PDB 1A03; 1A29; 1A2X; 1A75; 1AHR; 1AJ4; 1AJ5; 1AK8; 1ALV; 1ALW; 1AP4; 1AUI; » more