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PDOC00448

Ribonuclease III family signature and profile

Description:

Prokaryotic ribonuclease III (EC 3.1.26.3) (gene rnc) [1] is an enzyme that digests double-stranded RNA. It is involved in the processing of ribosomal RNA precursors and of some mRNAs. RNase III is evolutionary related [2] to the following proteins:

  • Fission yeast pac1, a ribonuclease that probably inhibits mating and meiosis by degrading a specific mRNA required for sexual development.
  • Yeast ribonuclease III (gene RNT1), a dsRNA-specific nuclease that cleaves eukaryotic preribosomal RNA at various sites.
  • Caenorhabditis elegans hypothetical protein F26E4.13.
  • Paramecium bursaria chlorella virus 1 protein A464R.
  • Synechocystis strain PCC 6803 hypothetical protein slr0346.
  • Fission yeast hypothetical protein SpAC8A4.08c, a protein with a N-terminal helicase domain and a C-terminal RNase III domain.
  • Caenorhabditis elegans hypothetical protein K12H4.8, a protein with the same structure as SpAC8A4.08c.

These proteins share regions of sequence similarity; one of which is a highly conserved stretch of 9 residues which we use as a signature pattern.

Last update:

December 2004 / Pattern and text revised.

Technical section:

PROSITE methods (with tools and information) covered by this documentation:

RNASE_3_2, PS50142; Ribonuclease III family domain profile  (MATRIX)
Sequences known to belong to this class detected by the profile: ALL
Other sequence(s) detected in Swiss-Prot: NONE.
• Domain architecture view of Swiss-Prot proteins matching PS50142
PS50142
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS50142
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS50142
• Scan Swiss-Prot/TrEMBL entries against PS50142
• view ligand binding statistics
Matching PDB structures: 1I4S 1JFZ 1O0W 1RC5 ... [ALL]
RNASE_3_1, PS00517; Ribonuclease III family signature  (PATTERN)
Consensus pattern: [DEQ] - [KRQT] - [LMF] - E - [FYW] - [LV] - G - D - [SARHG]
Sequences known to belong to this class detected by the pattern: ALL
Other sequence(s) detected in Swiss-Prot: NONE.
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00517
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00517
• Scan Swiss-Prot/TrEMBL entries against PS00517
• view ligand binding statistics
Matching PDB structures: 1I4S 1JFZ 1O0W 1RC5 ... [ALL]

References:

1 AuthorsNashimoto H., Uchida H.
TitleDNA sequencing of the Escherichia coli ribonuclease III gene and its mutations.
SourceMol. Gen. Genet. 201:25-29(1985).
PubMed ID3903434
2 AuthorsMian I.S.
TitleComparative sequence analysis of ribonucleases HII, III, II PH and D.
SourceNucleic Acids Res. 25:3187-3195(1997).
PubMed ID9241229

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