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PDOC00163

Ubiquitin-conjugating enzymes signature and profile

Description:

Ubiquitin-conjugating enzymes (EC 6.3.2.19) (UBC or E2 enzymes) [1,2,3] catalyze the covalent attachment of ubiquitin to target proteins. An activated ubiquitin moiety is transferred from an ubiquitin-activating enzyme (E1) to E2 which later ligates ubiquitin directly to substrate proteins with or without the assistance of 'N-end' recognizing proteins (E3).

In most species there are many forms of UBC (at least 9 in yeast) which are implicated in diverse cellular functions.

A cysteine residue is required for ubiquitin-thiolester formation. There is a single conserved cysteine in UBC's and the region around that residue is conserved in the sequence of known UBC isozymes. We have used that region as a signature pattern. We also developed a profile that spans the complete catalytical domain.

Expert(s) to contact by email:

Jentsch S.

Last update:

April 2006 / Pattern revised.

Technical section:

PROSITE methods (with tools and information) covered by this documentation:

UBIQUITIN_CONJUGAT_2, PS50127; Ubiquitin-conjugating enzymes family profile  (MATRIX)
Sequences known to belong to this class detected by the profile: ALL
Other sequence(s) detected in Swiss-Prot: NONE.
• Domain architecture view of Swiss-Prot proteins matching PS50127
PS50127
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS50127
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS50127
• Scan Swiss-Prot/TrEMBL entries against PS50127
• view ligand binding statistics
Matching PDB structures: 1A3S 1AYZ 1C4Z 1FBV ... [ALL]
UBIQUITIN_CONJUGAT_1, PS00183; Ubiquitin-conjugating enzymes active site  (PATTERN)
Consensus pattern: [FYWLSP] - H - [PC] - [NHL] - [LIV] - x(3,4) - G - x - [LIVP] - C - [LIV] - x(1,2) - [LIVR]
C is the active site residue
Sequences known to belong to this class detected by the pattern: ALL, except for yeast UBC6 (DOA2)
Other sequence(s) detected in Swiss-Prot: NONE.
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00183
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00183
• Scan Swiss-Prot/TrEMBL entries against PS00183
• view ligand binding statistics
Matching PDB structures: 1A3S 1AYZ 1C4Z 1FBV ... [ALL]

References:

1 AuthorsJentsch S., Seufert W., Sommer T., Reins H.-A.
TitleUbiquitin-conjugating enzymes: novel regulators of eukaryotic cells.
SourceTrends Biochem. Sci. 15:195-198(1990).
PubMed ID2193438
2 AuthorsJentsch S., Seufert W., Hauser H.-P.
TitleGenetic analysis of the ubiquitin system.
SourceBiochim. Biophys. Acta 1089:127-139(1991).
PubMed ID1647207
3 AuthorsHershko A.
TitleThe ubiquitin pathway for protein degradation.
SourceTrends Biochem. Sci. 16:265-268(1991).
PubMed ID1656558

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