PROSITE documentation PDOC00100Protein kinases signatures and profile
Eukaryotic protein kinases [1,2,3,4,5] are enzymes that belong to a very extensive family of proteins which share a conserved catalytic core common to both serine/threonine and tyrosine protein kinases. There are a number of conserved regions in the catalytic domain of protein kinases. We have selected two of these regions to build signature patterns. The first region, which is located in the N-terminal extremity of the catalytic domain, is a glycine-rich stretch of residues in the vicinity of a lysine residue, which has been shown to be involved in ATP binding. The second region, which is located in the central part of the catalytic domain, contains a conserved aspartic acid residue which is important for the catalytic activity of the enzyme [6]; we have derived two signature patterns for that region: one specific for serine/ threonine kinases and the other for tyrosine kinases. We also developed a profile which is based on the alignment in [1] and covers the entire catalytic domain.
Note:If a protein analyzed includes the two protein kinase signatures, the probability of it being a protein kinase is close to 100%
Note:Eukaryotic-type protein kinases have also been found in prokaryotes such as Myxococcus xanthus [11] and Yersinia pseudotuberculosis.
Note:The patterns shown above has been updated since their publication in [7].
Expert(s) to contact by email: Last update:April 2006 / Pattern revised.
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PURL: https://purl.expasy.org/prosite/documentation/PDOC00100
PROSITE methods (with tools and information) covered by this documentation:
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| Title | Protein kinases 6. The eukaryotic protein kinase superfamily: kinase (catalytic) domain structure and classification. | |
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| PubMed ID | 7768349 |
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| PubMed ID | 1835513 |
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| Title | Protein kinase catalytic domain sequence database: identification of conserved features of primary structure and classification of family members. | |
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| PubMed ID | 1956325 |
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| 5 | Authors | Hanks S.K. Quinn A.M. Hunter T. |
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| PubMed ID | 3291115 |
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| Title | Crystal structure of the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase. | |
| Source | Science 253:407-414(1991). | |
| PubMed ID | 1862342 |
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| Title | Sequence patterns in protein kinases. | |
| Source | Nature 331:22-22(1988). | |
| PubMed ID | 3340146 | |
| DOI | 10.1038/331022a0 |
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| Title | Evolutionary origin of aminoglycoside phosphotransferase resistance genes. | |
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| Source | Cell 67:995-1006(1991). |
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