| Official Name |
|---|
| Carbamoyl-phosphate synthase (glutamine-hydrolyzing).
|
| Alternative Name(s) |
|---|
| Carbamoyl phosphate synthetase. |
| Carbamoyl-phosphate synthetase (glutamine-hydrolyzing). |
| Carbamoylphosphate synthetase II. |
| Carbamyl phosphate synthetase (glutamine). |
| CPS. |
| GD-CPSase. |
| Glutamine-dependent carbamoyl-phosphate synthase. |
| Glutamine-dependent carbamyl phosphate synthetase. |
| Reaction catalysed |
|---|
| 2 ATP + L-glutamine + HCO(3)(-) + H(2)O <=> 2 ADP + phosphate + L-glutamate + carbamoyl phosphate |
| Comment(s) |
|---|
- The product carbamoyl phosphate is an intermediate in the
biosynthesis of arginine and the pyrimidine nucleotides.
- The enzyme from Escherichia coli has three separate active sites,
which are connected by a molecular tunnel that is almost 100 A in
length.
- The amidotransferase domain within the small subunit of the enzyme
hydrolyzes glutamine to ammonia via a thioester intermediate.
- The ammonia migrates through the interior of the protein, where it
reacts with carboxy phosphate to produce the carbamate intermediate.
- The carboxy-phosphate intermediate is formed by the phosphorylation
of bicarbonate by ATP at a site contained within the N-terminal half
of the large subunit.
- The carbamate intermediate is transported through the interior of the
protein to a second site within the C-terminal half of the large
subunit, where it is phosphorylated by another ATP to yield the final
product, carbamoyl phosphate.
- Formerly EC 2.7.2.9.
|
| Cross-references |
|---|
| Biochemical Pathways; map number(s) | G8 |
| PROSITE | PDOC00405 ; PDOC00676 ; PDOC50975 |
| BRENDA | 6.3.5.5 |
| PUMA2 | 6.3.5.5 |
| PRIAM enzyme-specific profiles | 6.3.5.5 |
| KEGG Ligand Database for Enzyme Nomenclature | 6.3.5.5 |
| IUBMB Enzyme Nomenclature | 6.3.5.5 |
| IntEnz | 6.3.5.5 |
| MEDLINE | Find literature relating to 6.3.5.5 |
| MetaCyc | 6.3.5.5 |
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