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NiceZyme View of ENZYME: EC 6.3.5.4

Accepted Name
Asparagine synthase (glutamine-hydrolyzing).
Alternative Name(s)
AS-B.
Asparagine synthetase (glutamine-hydrolyzing).
Asparagine synthetase B.
Glutamine-dependent asparagine synthetase.
Reaction catalysed
ATP + L-aspartate + L-glutamine + H(2)O <=> AMP + diphosphate + L-asparagine + L-glutamate
Comment(s)
  • The enzyme from Escherichia coli has two active sites that are connected by an intramolecular ammonia tunnel.
  • The enzyme catalyzes three distinct chemical reactions: glutamine hydrolysis to yield ammonia takes place in the N-terminal domain.
  • The C-terminal active site mediates both the synthesis of a beta- aspartyl-AMP intermediate and its subsequent reaction with ammonia.
  • The ammonia released is channeled to the other active site to yield asparagine.
Cross-references
PROSITEPDOC00406
BRENDA6.3.5.4
EC2PDB6.3.5.4
PRIAM enzyme-specific profiles6.3.5.4
KEGG Ligand Database for Enzyme Nomenclature6.3.5.4
IUBMB Enzyme Nomenclature6.3.5.4
IntEnz6.3.5.4
MEDLINEFind literature relating to 6.3.5.4
MetaCyc6.3.5.4
UniProtKB/Swiss-Prot
P54420, ASNB_BACSU;  P22106, ASNB_ECOLI;  Q58516, ASNH1_METJA;  
Q58456, ASNH2_METJA;  P42113, ASNH_BACSU;  P64248, ASNH_MYCBO;  
P64247, ASNH_MYCTU;  O05272, ASNO_BACSU;  P49092, ASNS1_LOTJA;  
P19251, ASNS1_PEA;  P49089, ASNS1_YEAST;  P49093, ASNS2_LOTJA;  
P19252, ASNS2_PEA;  P49090, ASNS2_YEAST;  P49078, ASNS_ARATH;  
P31752, ASNS_ASPOF;  Q1LZA3, ASNS_BOVIN;  P49091, ASNS_BRAOL;  
Q5ZJU3, ASNS_CHICK;  P19891, ASNS_CRIGR;  Q54MB4, ASNS_DICDI;  
P08243, ASNS_HUMAN;  P49094, ASNS_MAIZE;  P17714, ASNS_MESAU;  
Q5UQE1, ASNS_MIMIV;  Q61024, ASNS_MOUSE;  Q43011, ASNS_ORYSJ;  
Q5R6W9, ASNS_PONAB;  P49088, ASNS_RAT;  O24338, ASNS_SANAU;  
P78753, ASNS_SCHPO;  O24661, ASNS_TRIVS;  

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