| Accepted Name |
|---|
| [Skp1-protein]-hydroxyproline N-acetylglucosaminyltransferase.
|
| Alternative Name(s) |
|---|
| Skp1-HyPro GlcNAc-transferase. |
| UDP-GlcNAc:hydroxyproline polypeptide GlcNAc-transferase. |
| UDP-GlcNAc:Skp1-hydroxyproline GlcNAc-transferase. |
| UDP-N-acetylglucosamine (GlcNAc):hydroxyproline polypeptide GlcNAc-
transferase. |
| Reaction catalysed |
|---|
| UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline <=> UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline |
| Comment(s) |
|---|
- Requires dithiothreitol and a divalent cation for activity.
- This enzyme commences the building up of a pentasaccharide (Gal-
alpha-1-6Gal-alpha-1-L-Fuc-alpha-1-2Gal-beta-1-3GlcNAc) on Hyp-143 of
the Dictyostelium protein Skp1, which is required for the
ubiquitination of cell-cycle regulatory proteins and transcription
factors.
- The fucose residue is probably in the alpha configuration.
- The specificity of the enzyme for Skp1-Hyp-143 and its high affinity
for this substrate suggests that it is the GlcNAc-transferase that
modifies Skp1 in vivo.
|
| Cross-references |
|---|
| BRENDA | 2.4.1.229 |
| EC2PDB | 2.4.1.229 |
| PRIAM enzyme-specific profiles | 2.4.1.229 |
| KEGG Ligand Database for Enzyme Nomenclature | 2.4.1.229 |
| IUBMB Enzyme Nomenclature | 2.4.1.229 |
| IntEnz | 2.4.1.229 |
| MEDLINE | Find literature relating to 2.4.1.229 |
| MetaCyc | 2.4.1.229 |
| UniProtKB/Swiss-Prot |
|
ExPASy Home page