ProtParam

TITIN_HUMAN (Q8WZ42)

Titin (EC 2.7.11.1) (Connectin) (Rhabdomyosarcoma antigen MU-RMS-40.14)
Homo sapiens (Human)

The computation has been carried out on the complete sequence (34350 amino acids).

Warning: All computation results shown below do not take into account
any annotated post-translational modification.
 References and documentation are available.

Number of amino acids: 34350

Molecular weight: 3816030.05

Theoretical pI: 6.02

Amino acid composition: 
Ala (A)2084	  6.1%
Arg (R)1640	  4.8%
Asn (N)1111	  3.2%
Asp (D)1720	  5.0%
Cys (C) 513	  1.5%
Gln (Q) 942	  2.7%
Glu (E)3193	  9.3%
Gly (G)2066	  6.0%
His (H) 478	  1.4%
Ile (I)2062	  6.0%
Leu (L)2117	  6.2%
Lys (K)2943	  8.6%
Met (M) 398	  1.2%
Phe (F) 908	  2.6%
Pro (P)2517	  7.3%
Ser (S)2463	  7.2%
Thr (T)2546	  7.4%
Trp (W) 466	  1.4%
Tyr (Y) 999	  2.9%
Val (V)3184	  9.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 4913
Total number of positively charged residues (Arg + Lys): 4583

Atomic composition:

Carbon      C	    169719
Hydrogen    H	    270466
Nitrogen    N	     45688
Oxygen      O	     52238
Sulfur      S	       911

Formula: C169719H270466N45688O52238S911
Total number of atoms: 539022

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient  4083510
Abs 0.1% (=1 g/l)   1.070, assuming all pairs of Cys residues form cystines


Ext. coefficient  4051510
Abs 0.1% (=1 g/l)   1.062, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.38
This classifies the protein as unstable.



Aliphatic index: 80.40

Grand average of hydropathicity (GRAVY): -0.468