ProtParam

RRT14_YEAST (P40470)

Regulator of rDNA transcription protein 14
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

The computation has been carried out on the complete sequence (206 amino acids).

Warning: All computation results shown below do not take into account
any annotated post-translational modification.
 References and documentation are available.

Number of amino acids: 206

Molecular weight: 23845.14

Theoretical pI: 9.83

Amino acid composition: 
Ala (A)   6	  2.9%
Arg (R)  14	  6.8%
Asn (N)  12	  5.8%
Asp (D)  15	  7.3%
Cys (C)   1	  0.5%
Gln (Q)  13	  6.3%
Glu (E)  15	  7.3%
Gly (G)   8	  3.9%
His (H)   7	  3.4%
Ile (I)   7	  3.4%
Leu (L)  23	 11.2%
Lys (K)  32	 15.5%
Met (M)   1	  0.5%
Phe (F)   3	  1.5%
Pro (P)   5	  2.4%
Ser (S)  18	  8.7%
Thr (T)   9	  4.4%
Trp (W)   1	  0.5%
Tyr (Y)   3	  1.5%
Val (V)  13	  6.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 30
Total number of positively charged residues (Arg + Lys): 46

Atomic composition:

Carbon      C	      1033
Hydrogen    H	      1726
Nitrogen    N	       320
Oxygen      O	       322
Sulfur      S	         2

Formula: C1033H1726N320O322S2
Total number of atoms: 3403

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     9970
Abs 0.1% (=1 g/l)   0.418, assuming all pairs of Cys residues form cystines


Ext. coefficient     9970
Abs 0.1% (=1 g/l)   0.418, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.32
This classifies the protein as unstable.



Aliphatic index: 78.01

Grand average of hydropathicity (GRAVY): -1.176