ProtParam

RET4_RAT (P04916)

Retinol-binding protein 4 precursor (Plasma retinol-binding protein) (PRBP) (RBP)
Rattus norvegicus (Rat)

The computation has been carried out on the complete sequence (201 amino acids).

Warning: All computation results shown below do not take into account
any annotated post-translational modification.
 References and documentation are available.

Number of amino acids: 201

Molecular weight: 23220.10

Theoretical pI: 5.69

Amino acid composition: 
Ala (A)  15	  7.5%
Arg (R)  17	  8.5%
Asn (N)   8	  4.0%
Asp (D)  16	  8.0%
Cys (C)   6	  3.0%
Gln (Q)   8	  4.0%
Glu (E)  13	  6.5%
Gly (G)  13	  6.5%
His (H)   3	  1.5%
Ile (I)   6	  3.0%
Leu (L)  18	  9.0%
Lys (K)   9	  4.5%
Met (M)   4	  2.0%
Phe (F)  11	  5.5%
Pro (P)   5	  2.5%
Ser (S)  15	  7.5%
Thr (T)   9	  4.5%
Trp (W)   7	  3.5%
Tyr (Y)   7	  3.5%
Val (V)  11	  5.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 29
Total number of positively charged residues (Arg + Lys): 26

Atomic composition:

Carbon      C	      1028
Hydrogen    H	      1566
Nitrogen    N	       290
Oxygen      O	       307
Sulfur      S	        10

Formula: C1028H1566N290O307S10
Total number of atoms: 3201

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    49305
Abs 0.1% (=1 g/l)   2.123, assuming all pairs of Cys residues form cystines


Ext. coefficient    48930
Abs 0.1% (=1 g/l)   2.107, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.48
This classifies the protein as unstable.



Aliphatic index: 69.90

Grand average of hydropathicity (GRAVY): -0.515