ProtParam

TITIN_MOUSE (A2ASS6)

Titin (EC 2.7.11.1) (Connectin)
Mus musculus (Mouse)

The computation has been carried out on the complete sequence (35213 amino acids).

Warning: All computation results shown below do not take into account
any annotated post-translational modification.
 References and documentation are available.

Number of amino acids: 35213

Molecular weight: 3906487.63

Theoretical pI: 5.91

Amino acid composition: 
Ala (A)2239	  6.4%
Arg (R)1666	  4.7%
Asn (N)1105	  3.1%
Asp (D)1732	  4.9%
Cys (C) 514	  1.5%
Gln (Q) 971	  2.8%
Glu (E)3358	  9.5%
Gly (G)2081	  5.9%
His (H) 466	  1.3%
Ile (I)2033	  5.8%
Leu (L)2130	  6.0%
Lys (K)3038	  8.6%
Met (M) 423	  1.2%
Phe (F) 901	  2.6%
Pro (P)2709	  7.7%
Ser (S)2489	  7.1%
Thr (T)2621	  7.4%
Trp (W) 469	  1.3%
Tyr (Y)1003	  2.8%
Val (V)3265	  9.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 5090
Total number of positively charged residues (Arg + Lys): 4704

Atomic composition:

Carbon      C	    173643
Hydrogen    H	    276850
Nitrogen    N	     46726
Oxygen      O	     53583
Sulfur      S	       937

Formula: C173643H276850N46726O53583S937
Total number of atoms: 551739

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient  4106095
Abs 0.1% (=1 g/l)   1.051, assuming all pairs of Cys residues form cystines


Ext. coefficient  4073970
Abs 0.1% (=1 g/l)   1.043, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.05
This classifies the protein as unstable.



Aliphatic index: 79.35

Grand average of hydropathicity (GRAVY): -0.484